Microbiology Chapter 5 Quiz

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Questions and Answers

What is the primary structure of bacterial DNA?

  • Multiple linear chromosomes
  • Circular single-stranded structure
  • Single circular chromosome (correct)
  • Double stranded linear structure

Which of the following is NOT a characteristic of plasmids?

  • They can encode antibiotic resistance.
  • They are essential for bacterial growth. (correct)
  • They can be integrated into the chromosome.
  • They are circular, double-stranded DNA.

Which component is found in the eukaryotic nucleus?

  • Nucleolus (correct)
  • Ribosomes
  • Endospores
  • Plasmids

What type of cellular organization is typical of eukaryotic DNA?

<p>Multiple linear chromosomes (B)</p> Signup and view all the answers

Which statement correctly describes the membranes of eukaryotic cells?

<p>Eukaryotic cells contain membranous organelles. (A)</p> Signup and view all the answers

What is the role of Gram's iodine in the staining process?

<p>It stabilizes the dye by forming large complexes. (B)</p> Signup and view all the answers

What effect does alcohol have on gram-negative bacteria during the staining procedure?

<p>It removes the dye from the peptidoglycan layer. (D)</p> Signup and view all the answers

Which component of the cell wall allows gram-positive bacteria to retain the dye complex more effectively?

<p>A thicker peptidoglycan layer (C)</p> Signup and view all the answers

What is the purpose of applying safranin in the final step of the staining procedure?

<p>To stain the now colorless gram-negative bacteria. (C)</p> Signup and view all the answers

During the staining process, what happens to the outer membrane of gram-negative cells when alcohol is applied?

<p>It fractures and releases the dye complexes. (D)</p> Signup and view all the answers

What is a significant characteristic of Gram-positive bacterial cell walls?

<p>Presence of teichoic acids (D)</p> Signup and view all the answers

Which of the following correctly describes Gram-negative bacterial cell walls?

<p>Have a bilayer membrane with phospholipids (B)</p> Signup and view all the answers

What role does mycolic acid play in acid-fast bacteria?

<p>It helps the bacteria to survive desiccation (D)</p> Signup and view all the answers

During differential Gram staining, what is the first step performed?

<p>Applying crystal violet (B)</p> Signup and view all the answers

What appearance do Gram-negative bacteria display after the Gram staining procedure?

<p>Pink (A)</p> Signup and view all the answers

What is contained in the large periplasmic space of Gram-negative bacteria?

<p>Outer membrane components (A)</p> Signup and view all the answers

What is the function of the Lipid A portion of lipopolysaccharides in Gram-negative bacteria?

<p>Causes fever and inflammation (B)</p> Signup and view all the answers

Which statement about S-layers in archaea and bacteria is true?

<p>They are composed of a monomolecular layer of proteins (B)</p> Signup and view all the answers

What term describes the variation in size and shape of cells of a single bacterial species due to genetic and nutritional differences?

<p>Pleomorphism (A)</p> Signup and view all the answers

Which arrangement describes a pair of cocci bacteria?

<p>Diplococci (C)</p> Signup and view all the answers

What is the primary function of the slime layer glycocalyx in Streptococcus mutans?

<p>Attachment to surfaces (A)</p> Signup and view all the answers

What type of flagella arrangement has a single flagellum at one end of the bacterial cell?

<p>Monotrichous (C)</p> Signup and view all the answers

Which of the following is NOT a function of the glycocalyx?

<p>Photosynthesis (D)</p> Signup and view all the answers

Which structure is involved in adhesion to other cells and surfaces in bacteria?

<p>Fimbriae (B)</p> Signup and view all the answers

What is the primary characteristic of endospores?

<p>They are dormant and resistant cells. (B)</p> Signup and view all the answers

Which of the following correctly describes the life cycle of endospores?

<p>Dormant phase and vegetative phase (D)</p> Signup and view all the answers

What is the term used for a highly organized, tightly attached layer outside the bacterial cell wall?

<p>Capsule (B)</p> Signup and view all the answers

Which arrangement of bacilli refers to a chain of several cells?

<p>Streptobacilli (D)</p> Signup and view all the answers

Which domain of life does Clostridium belong to?

<p>Bacteria (B)</p> Signup and view all the answers

What type of flagella arrangement has flagella dispersed over the surface of the cell?

<p>Peritrichous (D)</p> Signup and view all the answers

Which of the following cellular components assist protozoans in movement?

<p>Pseudopodia (A)</p> Signup and view all the answers

Which type of bacterial arrangement consists of cells that remain partially attached by a small hinge region?

<p>Palisades (D)</p> Signup and view all the answers

What type of bacteria forms endospores?

<p>Some Gram-positive genera (B)</p> Signup and view all the answers

Which statement about saprophytic fungi is correct?

<p>They decompose dead organic matter. (B)</p> Signup and view all the answers

Flashcards

What is a plasmid?

A small, circular, double-stranded DNA molecule found in bacteria. It exists independently of the main chromosome and can replicate itself. Plasmids carry genes that are not essential for basic bacterial survival but provide additional traits like antibiotic resistance, toxin production, and metal tolerance.

What is bacterial chemotaxis?

The process by which bacteria move towards or away from chemical stimuli. It involves specialized sensory proteins that detect changes in the environment and trigger flagellar movement.

What is an endospore?

A dormant, highly resistant form of a bacterium that can survive harsh environmental conditions. It contains a core of genetic material surrounded by a thick, protective coat.

What are the main types of bacterial cell walls?

The cell wall of bacteria can be categorized into two main types: Gram-positive and Gram-negative. The difference lies in the structure and composition of the cell wall.

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What is the nucleus of a eukaryotic cell?

The nucleus is a membrane-bound organelle found in eukaryotic cells. It contains the cell's genetic material, DNA, in the form of chromosomes. The nucleus is responsible for controlling and regulating cellular activities.

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What is the role of Gram's iodine in Gram staining?

A chemical solution used in Gram staining that acts as a mordant, stabilizing the crystal violet dye within the peptidoglycan layer of bacterial cell walls. This results in larger dye complexes being trapped more effectively in thicker Gram-positive walls compared to thinner Gram-negative walls.

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What is the purpose of alcohol in Gram staining?

A decolorizing agent used in Gram staining that dissolves lipids in the outer membrane of Gram-negative bacteria. This weakens the cell wall, allowing the dye complexes to escape, leaving the cells colorless.

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Why is safranin used in Gram staining?

A counterstain used in Gram staining to colorize Gram-negative bacteria that have lost their dye during the decolorizing step. This helps to distinguish the Gram-negative cells from the Gram-positive ones.

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What is Gram staining?

A staining technique that differentiates bacteria into two main groups based on differences in their cell wall composition. Gram-positive bacteria retain the initial crystal violet dye, appearing purple. Gram-negative bacteria lose the dye after decolorization and are stained red by the counterstain safranin.

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What is the significance of peptidoglycan in Gram staining?

A layer of complex carbohydrates found in bacterial cell walls that plays a crucial role in determining the Gram staining result. It's thicker in Gram-positive bacteria, explaining their ability to retain the dye.

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What is a slime layer?

A loosely organized, attached layer of glycocalyx that surrounds some bacteria, aiding in attachment to surfaces, protecting against immune cells, and acting as a receptor for certain molecules.

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What are endospores?

A tough, dormant structure formed by certain bacteria to survive harsh conditions. They are highly resistant to heat, drying, freezing, radiation, and chemicals.

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What is sporulation in bacteria?

The process by which a vegetative bacterial cell transforms into an endospore. It involves a series of complex steps and allows the bacterium to preserve its genetic material.

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What is germination in bacteria?

The process by which an endospore returns to its vegetative state, allowing it to resume normal growth and division. This occurs when favorable conditions return.

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What are molds?

A form of fungi that consists of intertwining filaments called hyphae. These hyphae collectively form a network called mycelium.

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What are yeasts?

Unicellular fungi that propagate through budding or fission. They may form chains called pseudohyphae.

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What are saprophytes?

Organisms that obtain nutrients from dead, decomposing organic matter. They play a crucial role in breaking down complex materials.

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What is the nuclear envelope?

The nuclear envelope is a double membrane that encloses the nucleus, regulating the movement of molecules in and out. It is studded with nuclear pores, which act as channels for the transport of substances between the nucleus and the cytoplasm.

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What distinguishes Gram-positive bacteria?

Gram-positive bacteria have a thick layer of peptidoglycan in their cell wall, which stains purple in Gram staining. This thick layer provides structural support and protection.

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What distinguishes Gram-negative bacteria?

Gram-negative bacteria have a thin layer of peptidoglycan and an outer membrane containing lipopolysaccharide (LPS). This outer membrane is responsible for their pink staining in Gram staining.

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What are Mycoplasmas?

Mycoplasmas are unique bacteria that lack a cell wall, making them more susceptible to osmotic stress and requiring different survival strategies.

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What are Acid-fast bacteria?

Acid-fast bacteria have a high mycolic acid content in their cell walls. Mycolic acid provides resistance to desiccation (drying out) and helps these bacteria survive in harsh environments.

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What is lipopolysaccharide (LPS)?

Lipopolysaccharide (LPS) is a complex molecule found in the outer membrane of Gram-negative bacteria. Its lipid A component can trigger immune responses in the host, potentially leading to inflammation, fever, and even shock.

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What are Teichoic acids and Lipoteichoic acids?

Teichoic acids and lipoteichoic acids are unique molecules present in the cell walls of Gram-positive bacteria. They play a role in cell wall structure, adhesion, and immune recognition.

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What is the periplasmic space?

The periplasmic space is a region between the inner and outer membranes of Gram-negative bacteria. It contains various enzymes and transport proteins involved in nutrient uptake and detoxification.

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What is pleomorphism?

Variation in the size and shape of bacteria within a single species, often caused by factors like nutrition and genetics.

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What are bacterial arrangements?

Bacterial arrangements describe how individual cells group together.

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How do cocci bacteria arrange?

Cocci are spherical bacteria that can arrange in single cells, pairs (diplococci), groups of four (tetrads), irregular clusters (staphylococci or micrococci), or chains (streptococci).

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How do bacilli bacteria arrange?

Bacilli are rod-shaped bacteria that can arrange as single cells, pairs with ends attached (diplobacilli), chains (streptobacilli), or palisades, where chains remain linked at the ends like a fence.

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What are the different types of flagella arrangements?

Flagella are whip-like structures used for bacterial movement. They can be arranged differently, affecting movement speed and direction. Monotrichous: one flagellum, lophotrichous: a bunch of flagella, amphitrichous: flagella at both ends, peritrichous: flagella all over.

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What are fimbrae?

Fimbrae are fine, hair-like structures found on bacterial surfaces that help with attachment to other cells and surfaces, particularly in the formation of biofilms.

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What is a bacterial capsule?

A bacterial capsule is a highly organized, tightly attached protective layer external to the cell wall, made of sugars and proteins. It aids in resisting host immune systems and forming biofilms.

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What is a bacterial slime layer?

A bacterial slime layer is a loosely attached, unorganized layer external to the cell wall, composed of sugars and proteins. It provides some protection and aids in attachment.

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Study Notes

Exam 1 Review

  • The exam covers bacterial cell wall structure (Gram +, Gram -, Acid fast), bacterial cellular morphology and arrangement, prokaryotic and eukaryotic cell components, bacterial chemotaxis, endospores, fungi and protozoa, and taxonomy (Domains and Kingdoms).
  • Bacterial DNA is circular, double-stranded, and helical, represented by one chromosome.
  • Eukaryotic DNA is linear (mostly), double-stranded, and helical, represented by multiple chromosomes.
  • Plasmids are small, circular, double-stranded DNA molecules, often free or integrated into the chromosome. They are duplicated and passed on to offspring and may encode antibiotic resistance, tolerance to toxic metals, enzymes, and toxins. They are useful in genetic engineering, as they are readily manipulated and transferred between cells.
  • Eukaryotic cells include a nucleus, often the largest organelle, containing most of the cell's DNA. It's surrounded by a nuclear envelope with pores and contains a semiliquid nucleoplasm and chromatin. RNA components of ribosomes are synthesized in the nucleolus within the nucleoplasm.
  • Mitochondria have an outer and inner membrane, cristae, and a matrix containing ribosomes.
  • Gram-positive bacterial cell walls have a relatively thick peptidoglycan layer, with teichoic and lipoteichoic acids. Gram-positive bacteria appear purple after Gram staining.
  • Gram-negative bacterial cell walls have a thin peptidoglycan layer, an outer membrane containing LPS (lipopolysaccharide) with lipid A, and a periplasmic space between the inner and outer membranes. They appear pink after Gram staining, and lipid A can cause inflammation, shock, and blood clotting.
  • Many bacterial and archaeal types have S-layers outside the cell wall or outer membrane. These layers are a monomolecular layer of a protein (or in some cases, two identical proteins or glycoproteins).
  • Differential Gram staining involves 4 steps; Crystal violet, Gram's iodine, alcohol, and safranin (red dye) to distinguish between Gram-positive and Gram-negative bacteria.
  • Bacterial shapes and arrangements are varied; coccus (spherical), rod, curved. Pleomorphism is variation in size and shape of cells within a bacterial species. Arrangements can include single cells, pairs (diplococci), groups of four (tetrads), irregular clusters (staphylococci and micrococci), chains (streptococci), packets of 8-64 cells (sarcina), and chains of rod-shaped cells (streptobacilli), among others..
  • Bacteria move using flagella (monotrichous, lophotrichous, amphitrichous, and peritrichous). Spirochetes have endoflagella, which are confined within the periplasm between the inner and outer cell membranes.
  • Fimbrae are hair-like bristles on the cell surface used for adhesion to other cells or surfaces and biofilm formation.
  • Pili are used for bacterial conjugation, a form of bacterial mating.
  • Glycocalyx (capsule or slime layer) is a coating of molecules external to the cell wall, made of sugars and/or proteins. Capsules are highly organized, and slime layers are loosely organized and are involved in attachment and inhibit killing by white blood cells.
  • Chemotaxis is the movement of bacteria in response to a chemical signal. Bacteria exhibiting chemotaxis move towards attractants and away from repellents.
  • Taxonomy is the science of organizing, classifying, and naming living things. It involves domains (Archaea, Bacteria, Eukarya), kingdoms, phyla, classes, orders, families, genera, and species.
  • Endospores are dormant resting cells produced by some genera of Bacillus and Clostridium. They are the hardiest of all life forms, withstanding extremes in heat, drying, freezing, radiation, and harsh chemicals. Sporulation is the formation of the endospore, and germination is the return to a vegetative form. Examples of bacteria that form endospores include Clostridium tetani, Clostridium perfringens, Clostridium botulinum, Clostridium difficile, and Bacillus anthracis.
  • Protozoa (Kingdom Protista) use pseudopodia, flagella, or cilia to move.
  • Fungi include molds (intertwining filaments called hyphae forming mycelium) and yeasts (unicellular, budding or fission, may form pseudo-hyphae). They are saprophytic, meaning they live on dead, decomposing matter.

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