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Questions and Answers
What is the primary structure of bacterial DNA?
What is the primary structure of bacterial DNA?
- Multiple linear chromosomes
- Circular single-stranded structure
- Single circular chromosome (correct)
- Double stranded linear structure
Which of the following is NOT a characteristic of plasmids?
Which of the following is NOT a characteristic of plasmids?
- They can encode antibiotic resistance.
- They are essential for bacterial growth. (correct)
- They can be integrated into the chromosome.
- They are circular, double-stranded DNA.
Which component is found in the eukaryotic nucleus?
Which component is found in the eukaryotic nucleus?
- Nucleolus (correct)
- Ribosomes
- Endospores
- Plasmids
What type of cellular organization is typical of eukaryotic DNA?
What type of cellular organization is typical of eukaryotic DNA?
Which statement correctly describes the membranes of eukaryotic cells?
Which statement correctly describes the membranes of eukaryotic cells?
What is the role of Gram's iodine in the staining process?
What is the role of Gram's iodine in the staining process?
What effect does alcohol have on gram-negative bacteria during the staining procedure?
What effect does alcohol have on gram-negative bacteria during the staining procedure?
Which component of the cell wall allows gram-positive bacteria to retain the dye complex more effectively?
Which component of the cell wall allows gram-positive bacteria to retain the dye complex more effectively?
What is the purpose of applying safranin in the final step of the staining procedure?
What is the purpose of applying safranin in the final step of the staining procedure?
During the staining process, what happens to the outer membrane of gram-negative cells when alcohol is applied?
During the staining process, what happens to the outer membrane of gram-negative cells when alcohol is applied?
What is a significant characteristic of Gram-positive bacterial cell walls?
What is a significant characteristic of Gram-positive bacterial cell walls?
Which of the following correctly describes Gram-negative bacterial cell walls?
Which of the following correctly describes Gram-negative bacterial cell walls?
What role does mycolic acid play in acid-fast bacteria?
What role does mycolic acid play in acid-fast bacteria?
During differential Gram staining, what is the first step performed?
During differential Gram staining, what is the first step performed?
What appearance do Gram-negative bacteria display after the Gram staining procedure?
What appearance do Gram-negative bacteria display after the Gram staining procedure?
What is contained in the large periplasmic space of Gram-negative bacteria?
What is contained in the large periplasmic space of Gram-negative bacteria?
What is the function of the Lipid A portion of lipopolysaccharides in Gram-negative bacteria?
What is the function of the Lipid A portion of lipopolysaccharides in Gram-negative bacteria?
Which statement about S-layers in archaea and bacteria is true?
Which statement about S-layers in archaea and bacteria is true?
What term describes the variation in size and shape of cells of a single bacterial species due to genetic and nutritional differences?
What term describes the variation in size and shape of cells of a single bacterial species due to genetic and nutritional differences?
Which arrangement describes a pair of cocci bacteria?
Which arrangement describes a pair of cocci bacteria?
What is the primary function of the slime layer glycocalyx in Streptococcus mutans?
What is the primary function of the slime layer glycocalyx in Streptococcus mutans?
What type of flagella arrangement has a single flagellum at one end of the bacterial cell?
What type of flagella arrangement has a single flagellum at one end of the bacterial cell?
Which of the following is NOT a function of the glycocalyx?
Which of the following is NOT a function of the glycocalyx?
Which structure is involved in adhesion to other cells and surfaces in bacteria?
Which structure is involved in adhesion to other cells and surfaces in bacteria?
What is the primary characteristic of endospores?
What is the primary characteristic of endospores?
Which of the following correctly describes the life cycle of endospores?
Which of the following correctly describes the life cycle of endospores?
What is the term used for a highly organized, tightly attached layer outside the bacterial cell wall?
What is the term used for a highly organized, tightly attached layer outside the bacterial cell wall?
Which arrangement of bacilli refers to a chain of several cells?
Which arrangement of bacilli refers to a chain of several cells?
Which domain of life does Clostridium belong to?
Which domain of life does Clostridium belong to?
What type of flagella arrangement has flagella dispersed over the surface of the cell?
What type of flagella arrangement has flagella dispersed over the surface of the cell?
Which of the following cellular components assist protozoans in movement?
Which of the following cellular components assist protozoans in movement?
Which type of bacterial arrangement consists of cells that remain partially attached by a small hinge region?
Which type of bacterial arrangement consists of cells that remain partially attached by a small hinge region?
What type of bacteria forms endospores?
What type of bacteria forms endospores?
Which statement about saprophytic fungi is correct?
Which statement about saprophytic fungi is correct?
Flashcards
What is a plasmid?
What is a plasmid?
A small, circular, double-stranded DNA molecule found in bacteria. It exists independently of the main chromosome and can replicate itself. Plasmids carry genes that are not essential for basic bacterial survival but provide additional traits like antibiotic resistance, toxin production, and metal tolerance.
What is bacterial chemotaxis?
What is bacterial chemotaxis?
The process by which bacteria move towards or away from chemical stimuli. It involves specialized sensory proteins that detect changes in the environment and trigger flagellar movement.
What is an endospore?
What is an endospore?
A dormant, highly resistant form of a bacterium that can survive harsh environmental conditions. It contains a core of genetic material surrounded by a thick, protective coat.
What are the main types of bacterial cell walls?
What are the main types of bacterial cell walls?
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What is the nucleus of a eukaryotic cell?
What is the nucleus of a eukaryotic cell?
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What is the role of Gram's iodine in Gram staining?
What is the role of Gram's iodine in Gram staining?
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What is the purpose of alcohol in Gram staining?
What is the purpose of alcohol in Gram staining?
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Why is safranin used in Gram staining?
Why is safranin used in Gram staining?
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What is Gram staining?
What is Gram staining?
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What is the significance of peptidoglycan in Gram staining?
What is the significance of peptidoglycan in Gram staining?
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What is a slime layer?
What is a slime layer?
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What are endospores?
What are endospores?
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What is sporulation in bacteria?
What is sporulation in bacteria?
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What is germination in bacteria?
What is germination in bacteria?
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What are molds?
What are molds?
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What are yeasts?
What are yeasts?
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What are saprophytes?
What are saprophytes?
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What is the nuclear envelope?
What is the nuclear envelope?
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What distinguishes Gram-positive bacteria?
What distinguishes Gram-positive bacteria?
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What distinguishes Gram-negative bacteria?
What distinguishes Gram-negative bacteria?
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What are Mycoplasmas?
What are Mycoplasmas?
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What are Acid-fast bacteria?
What are Acid-fast bacteria?
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What is lipopolysaccharide (LPS)?
What is lipopolysaccharide (LPS)?
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What are Teichoic acids and Lipoteichoic acids?
What are Teichoic acids and Lipoteichoic acids?
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What is the periplasmic space?
What is the periplasmic space?
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What is pleomorphism?
What is pleomorphism?
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What are bacterial arrangements?
What are bacterial arrangements?
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How do cocci bacteria arrange?
How do cocci bacteria arrange?
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How do bacilli bacteria arrange?
How do bacilli bacteria arrange?
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What are the different types of flagella arrangements?
What are the different types of flagella arrangements?
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What are fimbrae?
What are fimbrae?
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What is a bacterial capsule?
What is a bacterial capsule?
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What is a bacterial slime layer?
What is a bacterial slime layer?
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Study Notes
Exam 1 Review
- The exam covers bacterial cell wall structure (Gram +, Gram -, Acid fast), bacterial cellular morphology and arrangement, prokaryotic and eukaryotic cell components, bacterial chemotaxis, endospores, fungi and protozoa, and taxonomy (Domains and Kingdoms).
- Bacterial DNA is circular, double-stranded, and helical, represented by one chromosome.
- Eukaryotic DNA is linear (mostly), double-stranded, and helical, represented by multiple chromosomes.
- Plasmids are small, circular, double-stranded DNA molecules, often free or integrated into the chromosome. They are duplicated and passed on to offspring and may encode antibiotic resistance, tolerance to toxic metals, enzymes, and toxins. They are useful in genetic engineering, as they are readily manipulated and transferred between cells.
- Eukaryotic cells include a nucleus, often the largest organelle, containing most of the cell's DNA. It's surrounded by a nuclear envelope with pores and contains a semiliquid nucleoplasm and chromatin. RNA components of ribosomes are synthesized in the nucleolus within the nucleoplasm.
- Mitochondria have an outer and inner membrane, cristae, and a matrix containing ribosomes.
- Gram-positive bacterial cell walls have a relatively thick peptidoglycan layer, with teichoic and lipoteichoic acids. Gram-positive bacteria appear purple after Gram staining.
- Gram-negative bacterial cell walls have a thin peptidoglycan layer, an outer membrane containing LPS (lipopolysaccharide) with lipid A, and a periplasmic space between the inner and outer membranes. They appear pink after Gram staining, and lipid A can cause inflammation, shock, and blood clotting.
- Many bacterial and archaeal types have S-layers outside the cell wall or outer membrane. These layers are a monomolecular layer of a protein (or in some cases, two identical proteins or glycoproteins).
- Differential Gram staining involves 4 steps; Crystal violet, Gram's iodine, alcohol, and safranin (red dye) to distinguish between Gram-positive and Gram-negative bacteria.
- Bacterial shapes and arrangements are varied; coccus (spherical), rod, curved. Pleomorphism is variation in size and shape of cells within a bacterial species. Arrangements can include single cells, pairs (diplococci), groups of four (tetrads), irregular clusters (staphylococci and micrococci), chains (streptococci), packets of 8-64 cells (sarcina), and chains of rod-shaped cells (streptobacilli), among others..
- Bacteria move using flagella (monotrichous, lophotrichous, amphitrichous, and peritrichous). Spirochetes have endoflagella, which are confined within the periplasm between the inner and outer cell membranes.
- Fimbrae are hair-like bristles on the cell surface used for adhesion to other cells or surfaces and biofilm formation.
- Pili are used for bacterial conjugation, a form of bacterial mating.
- Glycocalyx (capsule or slime layer) is a coating of molecules external to the cell wall, made of sugars and/or proteins. Capsules are highly organized, and slime layers are loosely organized and are involved in attachment and inhibit killing by white blood cells.
- Chemotaxis is the movement of bacteria in response to a chemical signal. Bacteria exhibiting chemotaxis move towards attractants and away from repellents.
- Taxonomy is the science of organizing, classifying, and naming living things. It involves domains (Archaea, Bacteria, Eukarya), kingdoms, phyla, classes, orders, families, genera, and species.
- Endospores are dormant resting cells produced by some genera of Bacillus and Clostridium. They are the hardiest of all life forms, withstanding extremes in heat, drying, freezing, radiation, and harsh chemicals. Sporulation is the formation of the endospore, and germination is the return to a vegetative form. Examples of bacteria that form endospores include Clostridium tetani, Clostridium perfringens, Clostridium botulinum, Clostridium difficile, and Bacillus anthracis.
- Protozoa (Kingdom Protista) use pseudopodia, flagella, or cilia to move.
- Fungi include molds (intertwining filaments called hyphae forming mycelium) and yeasts (unicellular, budding or fission, may form pseudo-hyphae). They are saprophytic, meaning they live on dead, decomposing matter.
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