Questions and Answers
What does the course adjustment knob on a microscope do?
The mechanical stage upper knob causes the stage to move?
The total magnification achieved using a 10 X objective lens with a 10 X eyepiece lens is 20 X.
False
The adaptive lens of a compound light microscope is attached to the?
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A wet mount requires the addition of certain dyes and cell fixatives approximately 12 hours before viewing the specimen.
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What is most likely to correct the problem of not obtaining a sharp image after rotating to a 100 X objective lens?
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Which items will you need to make a wet mount?
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Which objective lens provides the highest total magnification?
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What is the advantage of using a wet mount?
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Using oil immersion is useful because?
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A change in what component of microscopy influences the apparent size of the object being viewed?
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The mechanical stage lower knob causes the stage to move?
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Which objective lens provides the least total magnification?
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What does the fine adjustment knob on the microscope do?
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The _______ causes the stage to move upward and downward.
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In pond water, which of the following organisms are you most likely to find?
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What does the term motile mean?
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Study Notes
Microscope operation and components
- The course adjustment knob helps locate the focus plane of a specimen.
- The mechanical stage's upper knob moves the stage back-and-forth.
- The rotating nosepiece holds adaptive lenses for objective switching.
Magnification details
- Total magnification with a 10X objective and 10X eyepiece is 100X, not 20X.
- Oil immersion lens provides the highest total magnification and enhances resolving power.
- The scanning objective lens offers the least total magnification.
Wet mounts and specimen observations
- Wet mounts do not require dyes or cell fixatives added 12 hours prior.
- Advantages of wet mounts include viewing living cells and observing motility.
- Essential items for creating a wet mount include tweezers, a cover slip, a transfer pipette, and a clean slide.
Adjustments and focus
- Fine adjustment knob fine-tunes focus, ensuring a clear image of the specimen.
- If struggling to focus while using a 100X objective, adding immersion oil before repositioning it can help achieve a sharp image.
- The mechanical stage's lower knob moves the stage right and left for precise positioning.
Biological samples
- Pond water samples can contain bacteria, algae, rotifers, and protozoa but not human cells or viruses.
- The term "motile" indicates an organism's capability for movement.
Other microscopy considerations
- Light intensity and contrast do not affect apparent size but magnification does.
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Description
Test your knowledge on key concepts from Chapter 5 of Microbiology with these flashcards. This quiz covers important aspects of microscope use and movement related to laboratory procedures. Perfect for reinforcing your understanding of laboratory equipment and techniques.
