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Questions and Answers
What does the course adjustment knob on a microscope do?
What does the course adjustment knob on a microscope do?
- All these choices are correct
- Fine-tunes the focus
- Locates the focus plane (correct)
- Locates the specimen
The mechanical stage upper knob causes the stage to move?
The mechanical stage upper knob causes the stage to move?
- Right and left
- Back-and-forth (correct)
- All these choices are correct
- Up and down
The total magnification achieved using a 10 X objective lens with a 10 X eyepiece lens is 20 X.
The total magnification achieved using a 10 X objective lens with a 10 X eyepiece lens is 20 X.
False (B)
The adaptive lens of a compound light microscope is attached to the?
The adaptive lens of a compound light microscope is attached to the?
A wet mount requires the addition of certain dyes and cell fixatives approximately 12 hours before viewing the specimen.
A wet mount requires the addition of certain dyes and cell fixatives approximately 12 hours before viewing the specimen.
What is most likely to correct the problem of not obtaining a sharp image after rotating to a 100 X objective lens?
What is most likely to correct the problem of not obtaining a sharp image after rotating to a 100 X objective lens?
Which items will you need to make a wet mount?
Which items will you need to make a wet mount?
Which objective lens provides the highest total magnification?
Which objective lens provides the highest total magnification?
What is the advantage of using a wet mount?
What is the advantage of using a wet mount?
Using oil immersion is useful because?
Using oil immersion is useful because?
A change in what component of microscopy influences the apparent size of the object being viewed?
A change in what component of microscopy influences the apparent size of the object being viewed?
The mechanical stage lower knob causes the stage to move?
The mechanical stage lower knob causes the stage to move?
Which objective lens provides the least total magnification?
Which objective lens provides the least total magnification?
What does the fine adjustment knob on the microscope do?
What does the fine adjustment knob on the microscope do?
The _______ causes the stage to move upward and downward.
The _______ causes the stage to move upward and downward.
In pond water, which of the following organisms are you most likely to find?
In pond water, which of the following organisms are you most likely to find?
What does the term motile mean?
What does the term motile mean?
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Study Notes
Microscope operation and components
- The course adjustment knob helps locate the focus plane of a specimen.
- The mechanical stage's upper knob moves the stage back-and-forth.
- The rotating nosepiece holds adaptive lenses for objective switching.
Magnification details
- Total magnification with a 10X objective and 10X eyepiece is 100X, not 20X.
- Oil immersion lens provides the highest total magnification and enhances resolving power.
- The scanning objective lens offers the least total magnification.
Wet mounts and specimen observations
- Wet mounts do not require dyes or cell fixatives added 12 hours prior.
- Advantages of wet mounts include viewing living cells and observing motility.
- Essential items for creating a wet mount include tweezers, a cover slip, a transfer pipette, and a clean slide.
Adjustments and focus
- Fine adjustment knob fine-tunes focus, ensuring a clear image of the specimen.
- If struggling to focus while using a 100X objective, adding immersion oil before repositioning it can help achieve a sharp image.
- The mechanical stage's lower knob moves the stage right and left for precise positioning.
Biological samples
- Pond water samples can contain bacteria, algae, rotifers, and protozoa but not human cells or viruses.
- The term "motile" indicates an organism's capability for movement.
Other microscopy considerations
- Light intensity and contrast do not affect apparent size but magnification does.
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