Microbiology Assignment 6 Flashcards
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Questions and Answers

What results when a single bacterium reproduces?

Two genetically identical daughter cells

If you begin with six cells, how many cells would you have after three rounds of division?

forty-eight

Which of the following is not a step in bacterial cell division? (Select all that apply)

  • Cell elongation
  • Replication of the genetic material
  • Splitting apart of two new daughter cells
  • Disappearance of nuclear envelope (correct)
  • Which step of binary fission is the reason for genetically identical daughter cells?

    <p>Replication of the bacterial chromosome</p> Signup and view all the answers

    What enables the copied chromosomes to separate during binary fission?

    <p>The chromosomes are attached to different parts of the cell membrane, which elongates and thus separates the chromosomes.</p> Signup and view all the answers

    How long does it take for the daughter cells to initiate or start the next round of replication?

    <p>No time is required -- they are ready to divide immediately after DNA replication and separation of the daughter cells is complete if conditions are right.</p> Signup and view all the answers

    Put the following steps of bacterial replication in the correct order, starting from a parent cell: ______.

    <p>3, 1, 2, 4</p> Signup and view all the answers

    What would happen if the septum did not form during binary fission?

    <p>The parent cell would not have two copies of the chromosomes.</p> Signup and view all the answers

    Starting with three cells, how many cells would result from three rounds of replication?

    <p>twenty-four</p> Signup and view all the answers

    In the figure, which line best depicts a facultative anaerobe in the absence of O2?

    <p>Line B</p> Signup and view all the answers

    In the figure, which line best depicts a psychrotroph incubated at 0°C?

    <p>Line C</p> Signup and view all the answers

    Which of the following elements is NOT correctly matched with its cellular function? (Select all that apply)

    <p>Phosphorus: used for production of carbohydrates</p> Signup and view all the answers

    Salts and sugars work to preserve foods by creating a

    <p>hypertonic environment</p> Signup and view all the answers

    The term aerotolerant anaerobe refers to an organism that

    <p>does not use oxygen but tolerates it</p> Signup and view all the answers

    Most bacteria grow at pH

    <p>7</p> Signup and view all the answers

    Which of the following pairs of microbe classification terms and optimal growth temperatures is mismatched? (Select all that apply)

    <p>Thermophile: growth at 37°C</p> Signup and view all the answers

    What does facultative halophile refer to?

    <p>S.aureus</p> Signup and view all the answers

    Why do all cells need nitrogen?

    <p>For building amino acids, proteins, and nucleic acids</p> Signup and view all the answers

    Most pathogenic bacteria are thermophiles.

    <p>False</p> Signup and view all the answers

    Patients with indwelling catheters are susceptible to infections because

    <p>biofilms develop on catheters</p> Signup and view all the answers

    The majority of hospital-acquired infections are related to

    <p>biofilms</p> Signup and view all the answers

    Microbes in biofilms are less resistant to antimicrobials than are independent organisms.

    <p>False</p> Signup and view all the answers

    It is estimated that nearly ______ % of all infections are caused by biofilms.

    <p>70%</p> Signup and view all the answers

    Antimicrobials can be applied to the surface of catheters and IV tubing to prevent _______ _________.

    <p>biofilm development</p> Signup and view all the answers

    The biosafety level (BSL) for most introductory microbiology laboratories is

    <p>BSL-1</p> Signup and view all the answers

    A culture medium consisting of agar, peptone, and beef heart is a

    <p>complex medium</p> Signup and view all the answers

    For which of the following types of microbes would a microbiologist employ living host cells to support their growth?

    <p>Obligate intracellular organisms, such as chlamydias, rickettsias, and viruses</p> Signup and view all the answers

    Study Notes

    Bacterial Cell Division

    • A single bacterium reproduces by creating two genetically identical daughter cells.
    • Starting with six cells, after three rounds of division, there will be forty-eight cells.
    • Key steps in bacterial cell division include replication of genetic material, cell elongation, septum formation, and separation of daughter cells. The disappearance of the nuclear envelope is not involved.
    • The replication of the bacterial chromosome ensures that daughter cells are genetically identical.
    • Chromosomes separate during binary fission because they are attached to different cell membrane areas which elongate.

    Initiation of Division

    • Daughter cells are ready to initiate the next round of replication immediately after the previous round if conditions are favorable.
    • The correct order of bacterial replication is: chromosome replication, cell elongation, septum formation, and separation of daughter cells.

    Consequences of Faulty Division

    • If the septum does not form during binary fission, the parent cell will not have two chromosome copies.
    • Starting with three cells, three rounds of replication would yield twenty-four cells.

    Microbial Growth and Environmental Factors

    • A hypertonic environment is created by salts and sugars, helping to preserve foods.
    • Aerotolerant anaerobes do not use oxygen but can tolerate its presence.
    • The majority of bacteria prefer a neutral pH of 7 for optimal growth.

    Microbial Classification

    • Thermophiles are incorrectly matched with growth at 37°C; they thrive at higher temperatures.
    • S. aureus can be classified as a facultative halophile, indicating its tolerance to high salt concentrations.

    Nutritional Requirements

    • Cells require nitrogen for synthesizing amino acids, proteins, and nucleic acids.
    • Most pathogenic bacteria are not thermophiles, contrary to common assumptions.

    Infections and Biofilms

    • Indwelling catheters pose a risk for infections due to biofilm development on their surfaces.
    • Hospital-acquired infections are primarily associated with biofilms; approximately 70% of all infections are attributed to these structures.
    • Microbes in biofilms are more resistant to antimicrobials than independent organisms, challenging treatment efforts.

    Safety and Media in Microbiology

    • Most introductory microbiology labs operate at a biosafety level of BSL-1, indicating the lowest risk level.
    • A culture medium composed of agar, peptone, and beef heart is classified as a complex medium.
    • Obligate intracellular organisms, such as chlamydias, rickettsias, and viruses, require living host cells for cultivation.

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    Test your knowledge on the key concepts of bacterial cell reproduction and division with these flashcards. Ideal for students tackling Assignment 6 in Microbiology. Challenge yourself to answer questions about cell division processes and outcomes.

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