Microbiology: Antivirals and Antiseptics
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Questions and Answers

Which of the following statements about alcohol germicides is true?

  • Ethanol and isopropanol are two important types. (correct)
  • Alcohols require heat for germicidal effectiveness.
  • They have no impact on virus structures.
  • They are most effective in acidic environments.

Which method is suitable for sterilizing a liquid that cannot withstand heat?

  • Direct flame
  • UV light irradiation
  • Cold filtration (correct)
  • Alcohol immersion

What type of bacteria are halophilic bacteria?

  • Cold-loving bacteria
  • Heat-loving bacteria
  • Salt-loving bacteria (correct)
  • Acid-loving bacteria

What is the primary function of the enzyme lipase?

<p>To turn triglycerides into fatty acids and glycerol (D)</p> Signup and view all the answers

Which bacterium is commonly associated with gas gangrene?

<p>Clostridium perfringens (D)</p> Signup and view all the answers

What is the net gain of ATP from glycolysis?

<p>2 ATP (B)</p> Signup and view all the answers

Which compound serves as an inhibitor of nucleotide synthesis?

<p>Para-aminobenzoic acid (B)</p> Signup and view all the answers

Which of the following is considered a semisynthetic penicillin?

<p>Ampicillin (C)</p> Signup and view all the answers

What categorizes a microaerophilic organism?

<p>Requires lower levels of oxygen than atmospheric content (C)</p> Signup and view all the answers

Which of these methods is NOT considered a form of sterilization?

<p>Pasteurization (C)</p> Signup and view all the answers

Flashcards

Antiviral agents

Substances that prevent the infection of living tissue by microorganisms.

Substrate-Level Phosphorylation

ATP synthesis during fermentation.

Thermal death point

The lowest temperature at which a microbial population is killed in a specific time.

Binary Fission

The process by which bacteria reproduce.

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Pyrimidine

A nitrogenous base with a single ring.

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Minimum Inhibitory Concentration (MIC)

The lowest antibiotic concentration preventing microbial growth.

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Glycolysis Net ATP

The net gain of ATP molecules in the glycolysis process of cellular respiration is 2.

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Antimicrobial Growth Factor Analog

A compound that mimics a growth factor, disrupting its function and microbial growth.

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Pasteurization vs. Sterilization

Pasteurization reduces microorganisms; sterilization eliminates all living organisms.

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Pyruvic Acid (Krebs Cycle)

In the Krebs cycle, pyruvic acid from glycolysis is broken down to produce CO2, ATP, and NADH.

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Study Notes

Antiviral Agents

  • Antiviral agents are used to prevent infection of living tissue by microorganisms.

Antiseptics

  • Antiseptics are used to prevent microbial growth.

Cold Filtration

  • Cold filtration is a method used for sterilization of liquids that cannot be heated.

Alcohols as Germicides

  • Ethanol and isopropanol are the two most important alcohol germicides.

UV Light and Bacteria

  • UV light damages bacterial cells by affecting their DNA.

ATP Synthesis in Fermentation

  • ATP synthesis during fermentation occurs by substrate-level phosphorylation.

Chlorine in Municipal Water

  • Chlorine is used in municipal water to control bacterial populations.

Quaternary Ammonium Compounds

  • Quaternary ammonium compounds are cationic detergents that interact with cell membranes.

Antibiotics

  • Antibiotics are compounds produced by microorganisms that inhibit or kill other microorganisms.

Pyrimidine

  • A pyrimidine is a nitrogenous base with a single ring.

Penicillin and Gram Bacteria

  • Penicillin's effect is more apparent in Gram-positive bacteria.

Gas Gangrene Bacterium

  • Clostridium perfringens is associated with gas gangrene.

Mixed Acid Fermentation

  • Escherichia coli undergoes mixed acid fermentation.

Most Probable Number Method

  • The most probable number method is used when organisms cannot grow on agar.

Thermal Death Point

  • The thermal death point is the lowest temperature at which a microbial population is killed in a specific amount of time.

Psycrophile

  • A psychrophile is a cold-loving microbe.

Sterilization Methods

  • Direct flame is the most rapid method to achieve sterilization.

Barophilic Organisms

  • Barophilic organisms grow more rapidly at increased pressure.

Bacterial Reproduction

  • Bacteria reproduce by binary fission.

Aerobic Respiration and Final Hydrogen Acceptor

  • The final hydrogen acceptor in aerobic respiration is oxygen.

Halophilic Bacteria

  • Halophilic bacteria are salt-loving bacteria.

Hot Air Oven Sterilization

  • A two-hour period at 160 degrees is required for sterilization in a hot-air oven.

IMP and Nucleotide Precursors

  • IMP is a precursor of AMP and GMP.

Lipase Enzyme Action

  • Lipase turns triglycerides into glycerol and fatty acids.

Anaerobic Bacteria in Sewage

  • Methanococcus jannaschi is an anaerobic bacterium commonly found in sewage plants.

Antifungal Agents

  • Antifungal agents include clotrimazole, miconazole, amphotericin B, nystatin, griseofulvin, and flucytosine.

Semisynthetic Penicillins

  • Ampicillin and carbenicillin are semisynthetic penicillins.

Joseph Lister's Disinfectant

  • Phenol was used as a disinfectant by Joseph Lister in the 1860s.

Citric Acid Cycle (Krebs Cycle)

  • In the citric acid cycle, pyruvic acid from glycolysis is broken down into carbon dioxide, producing 2 ATP and NADH.

Minimum Inhibitory Concentration (MIC)

  • The minimum inhibitory concentration is the lowest concentration of an antimicrobial that results in no microbial growth.

Glycolysis ATP Gain

  • The net gain of ATP in glycolysis is 2 ATP.

Fermentable Compounds

  • Highly reduced molecules, hydrocarbons, and monosaccharides are compounds that can be fermented.

Nucleotide Synthesis Inhibitor

  • Para-aminobenzoic acid (PABA) inhibits nucleotide synthesis.

Central Fermentation Intermediate

  • Pyruvic acid is a central intermediate leading to various fermentation end products.

Sulfanilamide and Growth Factor

  • Sulfanilamide resembles the growth factor PABA.

Pasteurization vs. Sterilization

  • Pasteurization is not sterilization.

Aerobic Respiration Stages

  • Aerobic respiration involves glycolysis, the Krebs cycle, and the electron transport system.

Reactive Halogen Element

  • Fluorine is a reactive halogen element.

Microaerophilic Organisms

  • Microaerophilic organisms require oxygen, but at lower levels than atmospheric concentrations.

Batch Culture

  • A batch culture is a closed system with a single batch of medium.

Ionizing vs. Nonionizing Radiation

  • UV radiation is not an ionizing radiation.

Bacteria Removal Methods

  • Filtration is a method for removing bacteria through a porous substance.
  • Copper sulfate is used to kill algae.

Metabolic Pathway Inhibitor

  • A growth factor analog blocks a metabolic pathway.

Amylase Producing Organisms

  • Bacillus subtilis produces amylase

Chemotherapy

  • Chemotherapy uses drugs to harm invading organisms without harming the host.

Beta-galactosidase Containing Organism

  • Escherichia coli contains beta-galactosidase.

Butanediol Fermentation Organism

  • Enterobacter erogenous undergoes butanediol fermentation.

Macrolides

  • Macrolide antibiotics are not listed in the provided text.

Phosphoroclastic Bacteria

  • Phosphoroclastic bacteria are not defined in the provided text.

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Description

Test your knowledge on various antimicrobial agents, including antiviral agents, antiseptics, and alcohol germicides. Explore methods of sterilization like cold filtration and the role of chlorine in municipal water. This quiz also covers the basics of ATP synthesis in fermentation and the classification of antibiotics.

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