30 Questions
What type of bacteria are Vancomycin inhibitors selective for?
Gram-positive bacteria
Which of the following is an indicator used in culture media to detect acid or alkaline products?
pH indicators
What is the purpose of adding dyes and chemical substances like pH indicators in culture media?
To detect acid or alkaline products
Which antibiotic is specifically mentioned as an inhibitor for Proteus bacteria?
Colistin
What type of organic compounds do heterotrophs metabolize in the body?
Carbohydrates
What are the products when organic compounds are metabolized by bacteria?
Acids or alcohols
Which enrichment broth is specifically used for the growth of Salmonella while inhibiting other normal intestinal flora?
Selenite F Broth
What is the main purpose of Todd-Hewitt Broth?
Support the growth of Streptococci for serological grouping
Which agar is selective for Gram-negative Enteric bacilli and contains Eosin and Methylene Blue as differential indicators?
EMB Agar
In the context of bacteria categorization, what distinguishes lactose fermenters from non-lactose fermenters?
Utilization of lactose as a carbon source
If using an organic compound, what differentiates bacteria capable of fermentation from those capable of oxidation?
Ability to metabolize glucose
Why should Thioglycolate Broth be used solely in the isolation of anaerobic bacteria?
It can also grow aerobes and facultative anaerobes
Which type of bacteria does not produce pigment with or without light?
Non-Photochromogen
What is the purpose of adding inhibitor (Trimethroprim) to Proteus on Simmons Citrate Agar (SCA)?
To control the morphology of Neisseria
Which culture medium is specifically used for observation of hydrogen sulfide gas production, indole production, and motility?
Sulfide Indole Motility Medium (SIM)
Which bacteria are typically observed on Mueller Hinton Agar (MHA) with colonies on the surface of the medium?
Neisseria spp.
Which type of bacteria is capable of producing pigment when incubated without light?
Scotochromogen
In the context of AST, what does it mean when a bacterium is labeled as 'Susceptible'?
Capable of inhibiting bacteria
Which bacterial species can tolerate up to 10% salt concentration, except Vibrio cholerae and Vibrio mimicus?
Bacillus spp.
What is the degradation product of hemoglobin that serves as the X factor in culture media?
Hematin
Which type of culture media contains lysed RBC and has both X and V factors?
Chocolate Agar Plate
Why is the V factor not present in Blood Agar Plate (BAP) according to the text?
The RBC is intact, leading to NADase hydrolyzing the NAD (V factor).
How can the V factor be preserved in a culture medium that contains NADase, according to the text?
Applying physical methods such as heat to inactivate NADase.
Why does Chocolate Agar Plate (CAP) have both X and V factors available according to the text?
NADase is inactivated by physical methods like heat.
What is the solidifying agent commonly used in a culture medium?
Agar
Which medium is typically used for observation of hydrogen sulfide gas production, indole production, and motility?
Sulfide Indole Motility (SIM) Medium
Which type of culture medium is commonly used as a base in the preparation of other culture media?
Nutrient agar
What function does a primary media enriched with blood serve in microbiology?
To promote growth of fastidious microorganisms
Which chemicals are commonly used as inhibitors for gram-positive bacteria in culture media?
Sodium deoxycholate and thiosulfate
What is the purpose of a culture medium containing additives and supplements to support the growth of fastidious microorganisms?
To support the growth of fastidious microorganisms
Test your knowledge on antibiotics and dyes used in microbiology, including inhibitors for gram-negative bacteria and fungi, and indicators in culture media. Explore the properties and applications of various chemicals and antibiotics in microbial studies.
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