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Questions and Answers
What is the primary method of transmission for a disease caused by a pathogen that can cross the placenta?
What is the primary method of transmission for a disease caused by a pathogen that can cross the placenta?
Which of the following is NOT a portal of entry for microbial pathogens?
Which of the following is NOT a portal of entry for microbial pathogens?
Which of the following is an example of a fomite involved in indirect transmission?
Which of the following is an example of a fomite involved in indirect transmission?
What is the primary difference between brightfield and darkfield microscopy?
What is the primary difference between brightfield and darkfield microscopy?
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Which of the following factors is NOT considered a biological factor that influences the course of an infection?
Which of the following factors is NOT considered a biological factor that influences the course of an infection?
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Which of the following is NOT a recommended practice to reduce susceptibility to disease?
Which of the following is NOT a recommended practice to reduce susceptibility to disease?
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What is the causative agent of syphilis?
What is the causative agent of syphilis?
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Which of the following is NOT a common shape of bacteria?
Which of the following is NOT a common shape of bacteria?
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What is the primary function of teichoic acids in Gram-positive bacteria?
What is the primary function of teichoic acids in Gram-positive bacteria?
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Which of the following structures is NOT found in a typical bacterial cell?
Which of the following structures is NOT found in a typical bacterial cell?
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What is the main difference between passive and active transport across a cell membrane?
What is the main difference between passive and active transport across a cell membrane?
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Which of the following is a key feature of the Gram-negative outer membrane?
Which of the following is a key feature of the Gram-negative outer membrane?
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What is the primary function of flagella in bacteria?
What is the primary function of flagella in bacteria?
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What is the smallest distance by which two objects can be separated and still be distinguished?
What is the smallest distance by which two objects can be separated and still be distinguished?
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Which type of light microscopy uses a dark background to enhance the visibility of light-scattering objects?
Which type of light microscopy uses a dark background to enhance the visibility of light-scattering objects?
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Which of these is NOT a common bacterial shape?
Which of these is NOT a common bacterial shape?
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Which of the following accurately describes the interaction of light with an object in brightfield microscopy?
Which of the following accurately describes the interaction of light with an object in brightfield microscopy?
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Which of the following is NOT a type of light microscopy?
Which of the following is NOT a type of light microscopy?
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What type of interaction of light with an object is responsible for allowing us to capture surface details of microbes in microscopy?
What type of interaction of light with an object is responsible for allowing us to capture surface details of microbes in microscopy?
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Which type of light microscopy is particularly effective for observing unstained or thinly stained specimens?
Which type of light microscopy is particularly effective for observing unstained or thinly stained specimens?
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In which type of microscopy does the object block light and gain energy?
In which type of microscopy does the object block light and gain energy?
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Which of the following is NOT a characteristic of eukaryotic cells?
Which of the following is NOT a characteristic of eukaryotic cells?
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Which of the following processes does NOT require energy expenditure?
Which of the following processes does NOT require energy expenditure?
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What is the primary function of the cell membrane?
What is the primary function of the cell membrane?
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What is the main component of the bacterial cell wall?
What is the main component of the bacterial cell wall?
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Which of the following IS NOT a function of the cell membrane proteins?
Which of the following IS NOT a function of the cell membrane proteins?
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How does facilitated diffusion differ from simple diffusion?
How does facilitated diffusion differ from simple diffusion?
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Which process utilizes a transporter protein and modifies the transported substance as it crosses the membrane?
Which process utilizes a transporter protein and modifies the transported substance as it crosses the membrane?
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How do pathogens utilize ion gradients and ATP to acquire nutrients from their host?
How do pathogens utilize ion gradients and ATP to acquire nutrients from their host?
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Which of the following is NOT a component of the endomembrane system?
Which of the following is NOT a component of the endomembrane system?
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Which of the following cell types possess cell walls composed primarily of cellulose?
Which of the following cell types possess cell walls composed primarily of cellulose?
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What is the difference in size between prokaryotic and eukaryotic ribosomes?
What is the difference in size between prokaryotic and eukaryotic ribosomes?
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According to the endosymbiotic theory, what is the origin of mitochondria?
According to the endosymbiotic theory, what is the origin of mitochondria?
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Which of the following is NOT a piece of evidence supporting the endosymbiotic theory?
Which of the following is NOT a piece of evidence supporting the endosymbiotic theory?
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Which of the following is a characteristic of eukaryotic flagella?
Which of the following is a characteristic of eukaryotic flagella?
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Which bacterial organism is responsible for causing Lyme disease?
Which bacterial organism is responsible for causing Lyme disease?
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A distinctive symptom of Lyme disease is characterized by:
A distinctive symptom of Lyme disease is characterized by:
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Which of the following correctly describes prokaryotic cells?
Which of the following correctly describes prokaryotic cells?
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What is one key role that some microbes play in the environment?
What is one key role that some microbes play in the environment?
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What are the basic components that generally all cells contain?
What are the basic components that generally all cells contain?
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In scientific nomenclature, how should the genus and species names be formatted?
In scientific nomenclature, how should the genus and species names be formatted?
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Which of the following accurately describes the domain Archaea?
Which of the following accurately describes the domain Archaea?
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Which of the following statements about bacteria is true?
Which of the following statements about bacteria is true?
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How do viruses differ from living organisms?
How do viruses differ from living organisms?
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Which statement about eukaryotic microbes is accurate?
Which statement about eukaryotic microbes is accurate?
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Flashcards
Prokaryotic Cell
Prokaryotic Cell
A cell without a true nucleus; DNA located in nucleoid.
Eukaryotic Cell
Eukaryotic Cell
A cell with a true nucleus enclosed by a nuclear envelope.
Cell Membrane Functions
Cell Membrane Functions
Defines a cell, separates cytoplasm from the environment, supports structures, and regulates transport.
Passive Transport
Passive Transport
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Facilitated Diffusion
Facilitated Diffusion
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Active Transport
Active Transport
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Group Translocation
Group Translocation
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Pathogen Nutrient Acquisition
Pathogen Nutrient Acquisition
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Passive vs Active Transport
Passive vs Active Transport
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Gram-positive Cell Wall
Gram-positive Cell Wall
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Bacterial Flagella Motility
Bacterial Flagella Motility
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Structure of Eukaryotic Flagella
Structure of Eukaryotic Flagella
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Horizontal transmission
Horizontal transmission
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Vertical transmission
Vertical transmission
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Direct transmission
Direct transmission
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Indirect transmission
Indirect transmission
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Reservoir
Reservoir
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Asymptomatic carrier
Asymptomatic carrier
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Zoonotic diseases
Zoonotic diseases
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Portals of entry
Portals of entry
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Immersion oil
Immersion oil
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Total magnification
Total magnification
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Basic vs Acidic dyes
Basic vs Acidic dyes
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Gram stain
Gram stain
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Light microscopy types
Light microscopy types
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Magnification
Magnification
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Common bacteria shapes
Common bacteria shapes
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Staining requirement
Staining requirement
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Endomembrane system
Endomembrane system
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Rough endoplasmic reticulum (Rough ER)
Rough endoplasmic reticulum (Rough ER)
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Smooth endoplasmic reticulum (Smooth ER)
Smooth endoplasmic reticulum (Smooth ER)
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Prokaryotic ribosomes
Prokaryotic ribosomes
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Eukaryotic ribosomes
Eukaryotic ribosomes
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Endosymbiotic Theory
Endosymbiotic Theory
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Structure of flagella and cilia
Structure of flagella and cilia
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Borrelia burgdorferi
Borrelia burgdorferi
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Eukaryotic microbes
Eukaryotic microbes
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Microbial roles
Microbial roles
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Pathogenic microbes
Pathogenic microbes
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Cell structure
Cell structure
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Scientific nomenclature
Scientific nomenclature
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Three domains of life
Three domains of life
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Viruses vs living organisms
Viruses vs living organisms
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Study Notes
MBS326M Exam 1 Review
- The exam is in-class next Wednesday and has 40 multiple choice questions, lasting 60 minutes.
- This review covers previously taught learning objectives of the course material and is not a substitute for proper pre-exam study.
- The review focuses on what has been taught in the class, not what is specifically on the exam.
Chapter 1 Learning Objectives
- Microbiology is defined as "living organisms that require a microscope to be seen". Not all microbes are living, like viruses and prions.
- Microbes affect our lives by participating in nitrogen fixation, producing vitamins, and serving as primary producers.
- Microbes can be prokaryotic, eukaryotic or outside these categories. Prokaryotes include Archaea and Bacteria. Eukaryotes include fungi, protozoa and algae.
- Viruses are not considered living organisms; they are non-metabolic and have no cellular matter.
- The three major domains of life are: archaea, bacteria, and eukarya. Archaea and bacteria are prokaryotes. Eukarya are eukaryotes.
- There are common traits and differences in the domains of life. The domains each have different traits in their cells, how they reproduce, and whether or not they are pathogenic.
- Scientific nomenclature uses two names (Genus and species epithet) – genus is capitalized, and species epithet is lowercase and italicized
- Microbial diseases impacted human history and were observed by scientists like Hooke (~1665), van Leeuwenhoek (~1674), Jenner, Pasteur, Semmelweis, Lister, and Fleming.
- Koch’s postulates determine if a specific microbe causes a specific disease.
- Mitochondria and chloroplasts evolved through endosymbiosis.
Chapter 2 Learning Objectives
- The normal human microbiota is the collection of all microbes that live on and in the human body without causing disease under normal conditions.
- Pathogens are any bacterium, virus, fungus, protozoan, or worm (helminth) that causes diseases in humans.
- Colonization is the ability of a microbe to attach to the body surface and replicate. Adhesins are special proteins microbes use to attach to host cells. Parasites include ectoparasites (on the host's body) and endoparasites (inside host's body), e.g., worms, fleas, ticks, lice. Pathogenicity is the ability of an organism to cause disease.
- Pathogens can cause infections which do not always cause disease. Virulence is a degree of harm FOLLOWING an infection (severity). Pathogenicity is a specific disease caused by an organism.
- Differentiating between primary pathogens (cause disease in healthy hosts), and opportunistic pathogens (cause disease in compromised hosts). Primary pathogens are more likely to cause disease after infection in healthy individuals. Examples include those that cause rapid reproduction and high virulence. Opportunistic pathogens are less likely to cause disease in healthy hosts, and tend to have low virulence.
- Stages of infection: incubation, prodromal, illness, decline, and convalescence stages. Infections have arbitrary measures that can change based on the specific pathogen.
- Types of Disease Transmission: Direct Contact, Indirect Contact, Vehicle, Vector and Aerosol.
- Reservoirs are the animal, human, or environment that typically harbor the pathogen. Asymptomatic carriers harbor pathogens without disease. Zoonotic diseases are transmitted to humans and aren't always pathogenic in the animal reservoir.
- Portals of entry/exit include fecal-oral, skin, respiratory, urogenital, and parenteral routes. Parenteral is entry via breaks in the skin.
Chapter 3 Learning Objectives
- Units to measure microbes are typically micrometers (bacteria) and nanometers (viruses). Eukaryotes are generally larger than prokaryotes. Thiomargarita magnifica is the largest known bacterium.
- Magnification increases the apparent size of an image while resolution is the smallest distance by which objects can be separated and still distinguished.
- Brightfield microscopy is where the object absorbs light creating a bright background; Darkfield microscopy is where the object scatters light creating a dark background.
- Bacterial shapes are usually Bacillus (rod-shaped), Coccus (sphere-shaped), Vibrio (comma-shaped), Spirillum (corkscrew-shaped, thick, rigid), and Spirochete (corkscrew-shaped, thin, flexible).
- Light interacts with objects by absorption, reflection, refraction, and scattering.
- Immersion oil is necessary for higher magnifications (100x) to prevent light refraction so the light can enter the objective lens of the microscope.
- Types of light microscopy include: Compound Light Microscopy, Darkfield Microscopy, Phase-contrast Microscopy, Fluorescence Microscopy, Confocal Microscopy, and DIC Microscopy
Chapter 5 Learning Objectives
- Prokaryotes are distinct from eukaryotes, and have differences in their chromosomes, ribosomes and cell walls.
- Key parts of a bacterial cell are cell wall, cell membrane, outer membrane, cytoplasm, ribosomes, nucleoid and membrane proteins.
- Cell walls maintain cell shape, protect against osmotic shock and provide structural integrity.
- Bacterial cell membranes are phospholipid bilayers with proteins that selectively transport substances in/out the cell. Membrane proteins also enable cell signaling, facilitate movement of toxins/virulence factors and enable energy transfer.
- Eukaryotic cells have a nucleus and internal organelles, and are larger and more complex than prokaryotic cells.
- The endomembrane system is a system of interconnected membrane-bound organelles. Includes: Endoplasmic Reticulum (rough and smooth), Golgi apparatus, lysosomes, and the cell membrane.
- Endosymbiotic theory is that some organelles originated as smaller bacteria that were taken in and incorporated into larger bacterial cells. These bacteria evolve and are now present as organelles (i.e. mitochondria, chloroplasts).
- Structures like flagella (in eukaryotic cells), are composed of microtubules in a 9+2 arrangement, and cilia are appendages that only are found in eukaryotic cells.
- There are multiple structures of flagella distribution: monotrichous (one flagellum), amphitrichous (one flagellum per pole of the cell), lophotrichous (many flagella at one or both poles of cell), peritrichous (flagella spread over entire cell surface).
- Other types of bacterial motility include axial filaments (in spirochtes), twitching motility and gliding motility.
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Description
Test your knowledge on microbiology fundamentals, including pathogen transmission, bacterial structures, and microscopy techniques. This quiz explores critical concepts such as the causative agents of diseases, transmission routes, and the functions of various bacterial components.