Microbiology and Pathogens Quiz
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Questions and Answers

What is the primary method of transmission for a disease caused by a pathogen that can cross the placenta?

  • Direct Transmission
  • Vertical Transmission (correct)
  • Indirect Transmission
  • Vehicle Transmission
  • Which of the following is NOT a portal of entry for microbial pathogens?

  • Skin
  • Circulatory System (correct)
  • Digestive Tract
  • Respiratory Tract
  • Which of the following is an example of a fomite involved in indirect transmission?

  • Contaminated water
  • A mosquito
  • A used tissue (correct)
  • A contaminated needle
  • What is the primary difference between brightfield and darkfield microscopy?

    <p>Brightfield microscopy produces a bright image against a dark background, while darkfield microscopy produces a dark image against a bright background. (D)</p> Signup and view all the answers

    Which of the following factors is NOT considered a biological factor that influences the course of an infection?

    <p>Nutrition (A)</p> Signup and view all the answers

    Which of the following is NOT a recommended practice to reduce susceptibility to disease?

    <p>Sharing utensils (A)</p> Signup and view all the answers

    What is the causative agent of syphilis?

    <p>Treponema pallidum (B)</p> Signup and view all the answers

    Which of the following is NOT a common shape of bacteria?

    <p>Cube (B)</p> Signup and view all the answers

    What is the primary function of teichoic acids in Gram-positive bacteria?

    <p>To provide structural support to the cell wall (B)</p> Signup and view all the answers

    Which of the following structures is NOT found in a typical bacterial cell?

    <p>Endoplasmic reticulum (A)</p> Signup and view all the answers

    What is the main difference between passive and active transport across a cell membrane?

    <p>Active transport requires ATP, while passive transport does not. (A)</p> Signup and view all the answers

    Which of the following is a key feature of the Gram-negative outer membrane?

    <p>Presence of lipopolysaccharide (LPS) (C)</p> Signup and view all the answers

    What is the primary function of flagella in bacteria?

    <p>Motility and movement (B)</p> Signup and view all the answers

    What is the smallest distance by which two objects can be separated and still be distinguished?

    <p>Resolution (A)</p> Signup and view all the answers

    Which type of light microscopy uses a dark background to enhance the visibility of light-scattering objects?

    <p>Darkfield microscopy (B)</p> Signup and view all the answers

    Which of these is NOT a common bacterial shape?

    <p>Tetracoccus (A)</p> Signup and view all the answers

    Which of the following accurately describes the interaction of light with an object in brightfield microscopy?

    <p>Light is absorbed by the object, resulting in a dark image against a bright background. (D)</p> Signup and view all the answers

    Which of the following is NOT a type of light microscopy?

    <p>Electron microscopy (A)</p> Signup and view all the answers

    What type of interaction of light with an object is responsible for allowing us to capture surface details of microbes in microscopy?

    <p>Reflection (D)</p> Signup and view all the answers

    Which type of light microscopy is particularly effective for observing unstained or thinly stained specimens?

    <p>Darkfield microscopy (B)</p> Signup and view all the answers

    In which type of microscopy does the object block light and gain energy?

    <p>Brightfield microscopy (C)</p> Signup and view all the answers

    Which of the following is NOT a characteristic of eukaryotic cells?

    <p>Ribosomes with 70S sedimentation coefficient (D)</p> Signup and view all the answers

    Which of the following processes does NOT require energy expenditure?

    <p>Simple diffusion (C)</p> Signup and view all the answers

    What is the primary function of the cell membrane?

    <p>Controlling the movement of substances into and out of the cell (A)</p> Signup and view all the answers

    What is the main component of the bacterial cell wall?

    <p>Peptidoglycan (C)</p> Signup and view all the answers

    Which of the following IS NOT a function of the cell membrane proteins?

    <p>Synthesis of DNA and RNA (B)</p> Signup and view all the answers

    How does facilitated diffusion differ from simple diffusion?

    <p>Facilitated diffusion involves transporter proteins. (D)</p> Signup and view all the answers

    Which process utilizes a transporter protein and modifies the transported substance as it crosses the membrane?

    <p>Group translocation (A)</p> Signup and view all the answers

    How do pathogens utilize ion gradients and ATP to acquire nutrients from their host?

    <p>They use ion gradients to drive active transport of nutrients. (B)</p> Signup and view all the answers

    Which of the following is NOT a component of the endomembrane system?

    <p>Nucleus (C)</p> Signup and view all the answers

    Which of the following cell types possess cell walls composed primarily of cellulose?

    <p>Plant cells (C)</p> Signup and view all the answers

    What is the difference in size between prokaryotic and eukaryotic ribosomes?

    <p>Prokaryotic ribosomes are smaller than eukaryotic ribosomes. (C)</p> Signup and view all the answers

    According to the endosymbiotic theory, what is the origin of mitochondria?

    <p>Ingested aerobic bacteria (C)</p> Signup and view all the answers

    Which of the following is NOT a piece of evidence supporting the endosymbiotic theory?

    <p>Mitochondria and chloroplasts have 80S ribosomes (D)</p> Signup and view all the answers

    Which of the following is a characteristic of eukaryotic flagella?

    <p>Long and few appendages (C)</p> Signup and view all the answers

    Which bacterial organism is responsible for causing Lyme disease?

    <p>Borrelia burgdorferi (A)</p> Signup and view all the answers

    A distinctive symptom of Lyme disease is characterized by:

    <p>A bull's eye rash (D)</p> Signup and view all the answers

    Which of the following correctly describes prokaryotic cells?

    <p>They lack a nucleus and include organisms from the domains Bacteria and Archaea. (C)</p> Signup and view all the answers

    What is one key role that some microbes play in the environment?

    <p>Contributing to nitrogen fixation. (B)</p> Signup and view all the answers

    What are the basic components that generally all cells contain?

    <p>Genetic material, cell membrane, ribosomes, and cytoplasm. (D)</p> Signup and view all the answers

    In scientific nomenclature, how should the genus and species names be formatted?

    <p>The genus is capitalized and the species is in lowercase, both italicized. (B)</p> Signup and view all the answers

    Which of the following accurately describes the domain Archaea?

    <p>They live in extreme environments and are generally not pathogenic. (B)</p> Signup and view all the answers

    Which of the following statements about bacteria is true?

    <p>Bacteria are unicellular and also lack a true nucleus. (B)</p> Signup and view all the answers

    How do viruses differ from living organisms?

    <p>Viruses cannot reproduce without a host cell. (D)</p> Signup and view all the answers

    Which statement about eukaryotic microbes is accurate?

    <p>Eukaryotic microbes include organisms like fungi, protozoa, and algae. (D)</p> Signup and view all the answers

    Flashcards

    Prokaryotic Cell

    A cell without a true nucleus; DNA located in nucleoid.

    Eukaryotic Cell

    A cell with a true nucleus enclosed by a nuclear envelope.

    Cell Membrane Functions

    Defines a cell, separates cytoplasm from the environment, supports structures, and regulates transport.

    Passive Transport

    Movement of substances without energy; includes diffusion and osmosis.

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    Facilitated Diffusion

    Movement of solutes using a transporter protein in the membrane.

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    Active Transport

    Movement of substances against concentration gradient using energy (ATP).

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    Group Translocation

    Transport process altering substance as it crosses membrane, requires energy.

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    Pathogen Nutrient Acquisition

    Pathogens use ion gradients and ATP to extract nutrients from hosts.

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    Passive vs Active Transport

    Passive transport does not require energy; active transport does require energy to move substances against their gradient.

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    Gram-positive Cell Wall

    Thick peptidoglycan layer, retains crystal violet stain, provides structural support and protection.

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    Bacterial Flagella Motility

    Flagella enable movement and chemotaxis, allowing bacteria to respond to environmental changes.

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    Structure of Eukaryotic Flagella

    Eukaryotic flagella have a '9+2' microtubule arrangement for movement.

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    Horizontal transmission

    Infectious agent transferred from one person/animal to another.

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    Vertical transmission

    Infectious agent transferred from parent to offspring.

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    Direct transmission

    Organism spread directly from person to person.

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    Indirect transmission

    Organism spread through air, fomites, vehicles, or vectors.

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    Reservoir

    Animal, human, or environment that normally harbors the pathogen.

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    Asymptomatic carrier

    Person who harbors the disease agent but shows no symptoms.

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    Zoonotic diseases

    Infections of animals that can be transmitted to humans.

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    Portals of entry

    Routes through which microbial pathogens enter the body.

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    Immersion oil

    A special oil that reduces light refraction in microscopy.

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    Total magnification

    The product of the ocular lens magnification and the objective lens magnification.

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    Basic vs Acidic dyes

    Basic dyes have a positive charge and stain negatively charged structures; acidic dyes do the opposite.

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    Gram stain

    A differential stain that classifies bacteria into Gram-positive and Gram-negative based on cell wall properties.

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    Light microscopy types

    Includes brightfield and darkfield microscopy, differing by background and object appearance.

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    Magnification

    The process of enlarging the apparent size of an image.

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    Common bacteria shapes

    Bacteria can be shaped like rods (bacillus), spheres (coccus), commas (vibrio), corkscrews (spirillum/spirochete).

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    Staining requirement

    Fixation is needed before staining to preserve the specimen and enhance dye uptake.

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    Endomembrane system

    A system of membrane-bound organelles organizing transport and processing of lipids and proteins.

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    Rough endoplasmic reticulum (Rough ER)

    Membranous organelle studded with ribosomes, important for protein synthesis.

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    Smooth endoplasmic reticulum (Smooth ER)

    Membrane-bound organelle without ribosomes, involved in lipid synthesis and detoxification.

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    Prokaryotic ribosomes

    Smaller ribosomes (70S) found in prokaryotes, comprising 50S and 30S subunits.

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    Eukaryotic ribosomes

    Larger ribosomes (80S) found in eukaryotes, comprising 60S and 40S subunits.

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    Endosymbiotic Theory

    Theory proposing that mitochondria and chloroplasts originated from engulfed bacteria.

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    Structure of flagella and cilia

    Flagella are long and few; cilia are short and numerous, both made of microtubules in a 9+2 arrangement.

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    Borrelia burgdorferi

    Spirochete bacteria that causes Lyme disease, known for a characteristic rash.

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    Eukaryotic microbes

    Microbes with a nucleus, including fungi, protozoa, and algae.

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    Microbial roles

    Microbes are involved in nitrogen fixation, vitamin production, and serving as primary producers.

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    Pathogenic microbes

    Microbes that can cause diseases, historically significant since the 1850s as disease causes.

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    Cell structure

    The basic unit of life, enclosed by a membrane, contains genetic material, ribosomes, and cytoplasm.

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    Scientific nomenclature

    The naming system using genus and species, written in italicized format (e.g., Genus species).

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    Three domains of life

    Organisms are categorized into Archaea, Bacteria, and Eukarya, distinguished by cell structure and complexity.

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    Viruses vs living organisms

    Viruses are not classified as living organisms; they can only replicate inside host cells.

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    Study Notes

    MBS326M Exam 1 Review

    • The exam is in-class next Wednesday and has 40 multiple choice questions, lasting 60 minutes.
    • This review covers previously taught learning objectives of the course material and is not a substitute for proper pre-exam study.
    • The review focuses on what has been taught in the class, not what is specifically on the exam.

    Chapter 1 Learning Objectives

    • Microbiology is defined as "living organisms that require a microscope to be seen". Not all microbes are living, like viruses and prions.
    • Microbes affect our lives by participating in nitrogen fixation, producing vitamins, and serving as primary producers.
    • Microbes can be prokaryotic, eukaryotic or outside these categories. Prokaryotes include Archaea and Bacteria. Eukaryotes include fungi, protozoa and algae.
    • Viruses are not considered living organisms; they are non-metabolic and have no cellular matter.
    • The three major domains of life are: archaea, bacteria, and eukarya. Archaea and bacteria are prokaryotes. Eukarya are eukaryotes.
    • There are common traits and differences in the domains of life. The domains each have different traits in their cells, how they reproduce, and whether or not they are pathogenic.
    • Scientific nomenclature uses two names (Genus and species epithet) – genus is capitalized, and species epithet is lowercase and italicized
    • Microbial diseases impacted human history and were observed by scientists like Hooke (~1665), van Leeuwenhoek (~1674), Jenner, Pasteur, Semmelweis, Lister, and Fleming.
    • Koch’s postulates determine if a specific microbe causes a specific disease.
    • Mitochondria and chloroplasts evolved through endosymbiosis.

    Chapter 2 Learning Objectives

    • The normal human microbiota is the collection of all microbes that live on and in the human body without causing disease under normal conditions.
    • Pathogens are any bacterium, virus, fungus, protozoan, or worm (helminth) that causes diseases in humans.
    • Colonization is the ability of a microbe to attach to the body surface and replicate. Adhesins are special proteins microbes use to attach to host cells. Parasites include ectoparasites (on the host's body) and endoparasites (inside host's body), e.g., worms, fleas, ticks, lice. Pathogenicity is the ability of an organism to cause disease.
    • Pathogens can cause infections which do not always cause disease. Virulence is a degree of harm FOLLOWING an infection (severity). Pathogenicity is a specific disease caused by an organism.
    • Differentiating between primary pathogens (cause disease in healthy hosts), and opportunistic pathogens (cause disease in compromised hosts). Primary pathogens are more likely to cause disease after infection in healthy individuals. Examples include those that cause rapid reproduction and high virulence. Opportunistic pathogens are less likely to cause disease in healthy hosts, and tend to have low virulence.
    • Stages of infection: incubation, prodromal, illness, decline, and convalescence stages. Infections have arbitrary measures that can change based on the specific pathogen.
    • Types of Disease Transmission: Direct Contact, Indirect Contact, Vehicle, Vector and Aerosol.
    • Reservoirs are the animal, human, or environment that typically harbor the pathogen. Asymptomatic carriers harbor pathogens without disease. Zoonotic diseases are transmitted to humans and aren't always pathogenic in the animal reservoir.
    • Portals of entry/exit include fecal-oral, skin, respiratory, urogenital, and parenteral routes. Parenteral is entry via breaks in the skin.

    Chapter 3 Learning Objectives

    • Units to measure microbes are typically micrometers (bacteria) and nanometers (viruses). Eukaryotes are generally larger than prokaryotes. Thiomargarita magnifica is the largest known bacterium.
    • Magnification increases the apparent size of an image while resolution is the smallest distance by which objects can be separated and still distinguished.
    • Brightfield microscopy is where the object absorbs light creating a bright background; Darkfield microscopy is where the object scatters light creating a dark background.
    • Bacterial shapes are usually Bacillus (rod-shaped), Coccus (sphere-shaped), Vibrio (comma-shaped), Spirillum (corkscrew-shaped, thick, rigid), and Spirochete (corkscrew-shaped, thin, flexible).
    • Light interacts with objects by absorption, reflection, refraction, and scattering.
    • Immersion oil is necessary for higher magnifications (100x) to prevent light refraction so the light can enter the objective lens of the microscope.
    • Types of light microscopy include: Compound Light Microscopy, Darkfield Microscopy, Phase-contrast Microscopy, Fluorescence Microscopy, Confocal Microscopy, and DIC Microscopy

    Chapter 5 Learning Objectives

    • Prokaryotes are distinct from eukaryotes, and have differences in their chromosomes, ribosomes and cell walls.
    • Key parts of a bacterial cell are cell wall, cell membrane, outer membrane, cytoplasm, ribosomes, nucleoid and membrane proteins.
    • Cell walls maintain cell shape, protect against osmotic shock and provide structural integrity.
    • Bacterial cell membranes are phospholipid bilayers with proteins that selectively transport substances in/out the cell. Membrane proteins also enable cell signaling, facilitate movement of toxins/virulence factors and enable energy transfer.
    • Eukaryotic cells have a nucleus and internal organelles, and are larger and more complex than prokaryotic cells.
    • The endomembrane system is a system of interconnected membrane-bound organelles. Includes: Endoplasmic Reticulum (rough and smooth), Golgi apparatus, lysosomes, and the cell membrane.
    • Endosymbiotic theory is that some organelles originated as smaller bacteria that were taken in and incorporated into larger bacterial cells. These bacteria evolve and are now present as organelles (i.e. mitochondria, chloroplasts).
    • Structures like flagella (in eukaryotic cells), are composed of microtubules in a 9+2 arrangement, and cilia are appendages that only are found in eukaryotic cells.
    • There are multiple structures of flagella distribution: monotrichous (one flagellum), amphitrichous (one flagellum per pole of the cell), lophotrichous (many flagella at one or both poles of cell), peritrichous (flagella spread over entire cell surface).
    • Other types of bacterial motility include axial filaments (in spirochtes), twitching motility and gliding motility.

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