Microbiology Aerobes and Anaerobes Quiz

Which type of bacteria require oxygen?

Which type of anaerobe cannot tolerate oxygen?

Aerobes make use of ______.

Aerotolerant bacteria can utilize oxygen.

What does sodium thioglycollate do in Thioglycollate broth?

What color indicates the presence of oxygen in Thioglycollate broth?

What is the purpose of a Candle Jar?

What is the primary method used to create an anaerobic environment in an Anaerobic Jar?

At what temperature should the cultures be incubated for 24 hours?

In Thioglycollate broth, where are obligate anaerobes typically found?

Oxygen Requirements

Bacteria are categorized into groups based on oxygen needs.
Aerobes utilize oxygen in their metabolism and electron transport chain.
Obligate aerobes require oxygen for survival.
Microaerophiles prefer low oxygen and high carbon dioxide levels.

Anaerobes

Anaerobes do not utilize oxygen.
Obligate anaerobes cannot tolerate oxygen.
Aerotolerant anaerobes can tolerate oxygen but do not use it.
Facultative anaerobes can live with or without oxygen but prefer it.

Culturing Anaerobes

Thioglycollate Broth: contains a reducing agent and agar which reduces oxygen diffusion. Resazurin is an oxygen indicator. Pink color indicates presence of oxygen.
Candle Jar: candle consumes oxygen, produces CO2, suitable for microaerophiles.
Anaerobic Jar: creates an anaerobic environment by absorbing oxygen and generating CO2. Contains an oxygen indicator. Purple color indicates aerobic environment and pink indicates anaerobic.

Procedure for Thioglycollate Broth

Label the broth.
Aseptically inoculate one loop of culture.
Incubate at 37°C for 24 hours.

Procedure for Candle Jar and Anaerobic Jar

Label TS plates in 4 sections. Label the plates.
Label another TSA plate as described in Step 1.
Label another TSA plate as described in Step 1.
Aseptically streak cultures into labeled sections.
Place C-plate in candle jar. Incubate at 37°C for 24 hours.
Place A-plate in the anaerobic jar. Incubate at 37°C for 24 hours.

Test your knowledge on the different types of bacteria, particularly focusing on aerobes and anaerobes. This quiz covers essential concepts such as oxygen requirements, methodologies for creating anaerobic environments, and specific growth mediums like Thioglycollate broth. It's perfect for students of microbiology looking to reinforce their understanding.