Microbiology Aerobes and Anaerobes Quiz
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Questions and Answers

Which type of bacteria require oxygen?

  • Obligate aerobes (correct)
  • Obligate anaerobes
  • Microaerophiles (correct)
  • Aerotolerant

Which type of anaerobe cannot tolerate oxygen?

  • Facultative anaerobe
  • Microaerophile
  • Obligate anaerobe (correct)
  • Aerotolerant

Aerobes make use of ______.

oxygen

Aerotolerant bacteria can utilize oxygen.

<p>False (B)</p> Signup and view all the answers

What does sodium thioglycollate do in Thioglycollate broth?

<p>It reduces free oxygen to water.</p> Signup and view all the answers

What color indicates the presence of oxygen in Thioglycollate broth?

<p>Pink (C)</p> Signup and view all the answers

What is the purpose of a Candle Jar?

<p>To consume oxygen and generate carbon dioxide.</p> Signup and view all the answers

What is the primary method used to create an anaerobic environment in an Anaerobic Jar?

<p>Absorbing oxygen (B)</p> Signup and view all the answers

At what temperature should the cultures be incubated for 24 hours?

<p>37 degrees Celsius</p> Signup and view all the answers

In Thioglycollate broth, where are obligate anaerobes typically found?

<p>At the bottom (B)</p> Signup and view all the answers

Flashcards

Obligate aerobe

A bacterium that requires oxygen for metabolism and growth.

Microaerophile

A bacterium that prefers low oxygen levels and high carbon dioxide levels.

Obligate anaerobe

A bacterium that cannot tolerate oxygen and will die in its presence.

Aerotolerant anaerobe

A bacterium that cannot use oxygen for growth, but can tolerate its presence.

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Facultative anaerobe

A bacterium that can grow with or without oxygen, but prefers oxygen.

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Thioglycollate Broth

A liquid medium used to culture bacteria with varying oxygen requirements.

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Resazurin

A dye used as an oxygen indicator in thioglycollate broth.

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Candle Jar

A method to create an anaerobic environment by using a candle to reduce oxygen levels and increase CO2.

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Anaerobic Jar

A closed jar with a sachet that absorbs oxygen and generates carbon dioxide to create an anaerobic environment.

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Methylene blue

An oxygen indicator often used in anaerobic jars, showing oxygen presence with a purple color, anaerobiosis with pink.

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Study Notes

Oxygen Requirements

  • Bacteria are categorized into groups based on oxygen needs.
  • Aerobes utilize oxygen in their metabolism and electron transport chain.
  • Obligate aerobes require oxygen for survival.
  • Microaerophiles prefer low oxygen and high carbon dioxide levels.

Anaerobes

  • Anaerobes do not utilize oxygen.
  • Obligate anaerobes cannot tolerate oxygen.
  • Aerotolerant anaerobes can tolerate oxygen but do not use it.
  • Facultative anaerobes can live with or without oxygen but prefer it.

Culturing Anaerobes

  • Thioglycollate Broth: contains a reducing agent and agar which reduces oxygen diffusion. Resazurin is an oxygen indicator. Pink color indicates presence of oxygen.
  • Candle Jar: candle consumes oxygen, produces CO2, suitable for microaerophiles.
  • Anaerobic Jar: creates an anaerobic environment by absorbing oxygen and generating CO2. Contains an oxygen indicator. Purple color indicates aerobic environment and pink indicates anaerobic.

Procedure for Thioglycollate Broth

  • Label the broth.
  • Aseptically inoculate one loop of culture.
  • Incubate at 37°C for 24 hours.

Procedure for Candle Jar and Anaerobic Jar

  • Label TS plates in 4 sections. Label the plates.
  • Label another TSA plate as described in Step 1.
  • Label another TSA plate as described in Step 1.
  • Aseptically streak cultures into labeled sections.
  • Place C-plate in candle jar. Incubate at 37°C for 24 hours.
  • Place A-plate in the anaerobic jar. Incubate at 37°C for 24 hours.

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Description

Test your knowledge on the different types of bacteria, particularly focusing on aerobes and anaerobes. This quiz covers essential concepts such as oxygen requirements, methodologies for creating anaerobic environments, and specific growth mediums like Thioglycollate broth. It's perfect for students of microbiology looking to reinforce their understanding.

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