Microbiology: Acid-Fast Staining Process

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10 Questions

What is the name of the microorganism with the following characteristics: Shape - Cocci, Arrangement - irregular clusters, Colour - Violet, Gram's reaction - Gram's +ve?

Staphylococci

What is the correct arrangement of cocci bacteria that divide in 3 planes?

Clusters

Which of the following is a common error that can occur during Gram staining?

Decolorizing for too long

What is the recommended age of the culture for a valid Gram staining procedure?

18-24 hours old

Which of the following is a characteristic of the cell wall of Mycobacteria?

It is composed of a waxy, lipid-rich layer

What is the purpose of using control cultures during the Gram staining procedure?

To confirm the Gram reaction of the unknown sample

What is the most likely cause of a Gram positive bacterium appearing Gram negative during the staining procedure?

Decolorizing for too long

Which of the following is the correct Gram reaction and name for the microorganism with the following characteristics: Shape - Rods, Arrangement - Single, Color - red, Gram's reaction - Gram's –ve?

Gram negative Bacilli

What is the likely cause of a crystal violet precipitate being observed on an epithelial cell during Gram staining?

Confusion with Gram positive cocci

Which of the following is a characteristic of the cell division pattern in Streptococcus bacteria?

Division in two planes

This quiz covers the acid-fast staining process used in microbiology to identify bacteria such as coli rods, corynebacterium rods, actinomyces rods, vibrio rods, and spore formers. It also includes information on the Ziel-Neelsen method and acid-fast reagents like carbolfuchsin. Test your knowledge on this important staining technique!

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