Microbiology: Acid-Fast Staining Process
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Questions and Answers

What is the name of the microorganism with the following characteristics: Shape - Cocci, Arrangement - irregular clusters, Colour - Violet, Gram's reaction - Gram's +ve?

  • Bacillus
  • Staphylococci (correct)
  • Gram negative bacilli
  • Candida
  • What is the correct arrangement of cocci bacteria that divide in 3 planes?

  • Pairs
  • Single
  • Clusters (correct)
  • Chains
  • Which of the following is a common error that can occur during Gram staining?

  • Using a young culture
  • Using fresh reagents
  • Using a thin smear
  • Decolorizing for too long (correct)
  • What is the recommended age of the culture for a valid Gram staining procedure?

    <p>18-24 hours old</p> Signup and view all the answers

    Which of the following is a characteristic of the cell wall of Mycobacteria?

    <p>It is composed of a waxy, lipid-rich layer</p> Signup and view all the answers

    What is the purpose of using control cultures during the Gram staining procedure?

    <p>To confirm the Gram reaction of the unknown sample</p> Signup and view all the answers

    What is the most likely cause of a Gram positive bacterium appearing Gram negative during the staining procedure?

    <p>Decolorizing for too long</p> Signup and view all the answers

    Which of the following is the correct Gram reaction and name for the microorganism with the following characteristics: Shape - Rods, Arrangement - Single, Color - red, Gram's reaction - Gram's –ve?

    <p>Gram negative Bacilli</p> Signup and view all the answers

    What is the likely cause of a crystal violet precipitate being observed on an epithelial cell during Gram staining?

    <p>Confusion with Gram positive cocci</p> Signup and view all the answers

    Which of the following is a characteristic of the cell division pattern in Streptococcus bacteria?

    <p>Division in two planes</p> Signup and view all the answers

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