Microbiology: Acid-Fast Staining Process

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Questions and Answers

What is the name of the microorganism with the following characteristics: Shape - Cocci, Arrangement - irregular clusters, Colour - Violet, Gram's reaction - Gram's +ve?

  • Bacillus
  • Staphylococci (correct)
  • Gram negative bacilli
  • Candida

What is the correct arrangement of cocci bacteria that divide in 3 planes?

  • Pairs
  • Single
  • Clusters (correct)
  • Chains

Which of the following is a common error that can occur during Gram staining?

  • Using a young culture
  • Using fresh reagents
  • Using a thin smear
  • Decolorizing for too long (correct)

What is the recommended age of the culture for a valid Gram staining procedure?

<p>18-24 hours old (C)</p> Signup and view all the answers

Which of the following is a characteristic of the cell wall of Mycobacteria?

<p>It is composed of a waxy, lipid-rich layer (C)</p> Signup and view all the answers

What is the purpose of using control cultures during the Gram staining procedure?

<p>To confirm the Gram reaction of the unknown sample (B)</p> Signup and view all the answers

What is the most likely cause of a Gram positive bacterium appearing Gram negative during the staining procedure?

<p>Decolorizing for too long (C)</p> Signup and view all the answers

Which of the following is the correct Gram reaction and name for the microorganism with the following characteristics: Shape - Rods, Arrangement - Single, Color - red, Gram's reaction - Gram's –ve?

<p>Gram negative Bacilli (C)</p> Signup and view all the answers

What is the likely cause of a crystal violet precipitate being observed on an epithelial cell during Gram staining?

<p>Confusion with Gram positive cocci (A)</p> Signup and view all the answers

Which of the following is a characteristic of the cell division pattern in Streptococcus bacteria?

<p>Division in two planes (A)</p> Signup and view all the answers

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