Microbial Nutrients and Uptake

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Questions and Answers

Which nutrient category includes carbon, nitrogen, and sulfur?

  • Trace elements
  • Enzymatic factors
  • Macronutrients (correct)
  • Micronutrients

What is the primary energy source for phototrophs?

  • Chemical compounds
  • Organic molecules
  • Inorganic elements
  • Light (correct)

Which process utilizes energy to move substances against their concentration gradient?

  • Facilitated diffusion
  • Passive diffusion
  • Osmosis
  • Active transport (correct)

Which of the following is a key principle of bioenergetics?

<p>Thermodynamics laws (A)</p> Signup and view all the answers

What type of microorganisms obtain energy from organic compounds?

<p>Chemoorganotrophs (C)</p> Signup and view all the answers

What effect do competitive inhibitors have on enzyme activity?

<p>They compete with substrates for the active site (B)</p> Signup and view all the answers

Which component of microbial nutrition includes manganese and zinc?

<p>Trace elements (D)</p> Signup and view all the answers

Which term describes the movement of small molecules across the cell membrane without assistance?

<p>Passive diffusion (D)</p> Signup and view all the answers

What is the primary function of group translocation in cellular transport?

<p>To modify the transported molecule chemically (D)</p> Signup and view all the answers

Which of the following statements correctly describes redox reactions?

<p>They involve the transfer of electrons from oxidized compounds to reduced compounds. (D)</p> Signup and view all the answers

Which compound is known for storing and transferring energy within cells?

<p>ATP (B)</p> Signup and view all the answers

What is created as protons are pumped across the membrane during electron transport?

<p>Proton motive force (PMF) (B)</p> Signup and view all the answers

Which metabolic process is employed by organisms in the absence of oxygen?

<p>Fermentation (C)</p> Signup and view all the answers

What is substrate-level phosphorylation?

<p>A mechanism for ATP synthesis independent of electron transport (B)</p> Signup and view all the answers

What is the role of ATP synthase in cellular processes?

<p>To synthesize ATP using the proton motive force (A)</p> Signup and view all the answers

What does anaerobic respiration use to regenerate NAD+?

<p>Alternative electron acceptors (B)</p> Signup and view all the answers

What does glycolysis yield as a direct product?

<p>ATP and NADH (A)</p> Signup and view all the answers

What are the end products of anaerobic respiration?

<p>Ethanol and lactic acid (A)</p> Signup and view all the answers

What is the main purpose of the citric acid cycle?

<p>To generate NADH and FADH2 (B)</p> Signup and view all the answers

Which pathway is involved in synthesizing sugars from non-carbohydrate precursors?

<p>Gluconeogenesis (A)</p> Signup and view all the answers

What role do electron carriers like NADH and FADH2 play?

<p>They donate electrons to the electron transport chain (C)</p> Signup and view all the answers

Which molecules are synthesized from acetyl-CoA?

<p>Fatty acids (A)</p> Signup and view all the answers

What is a primary function of polysaccharides in microorganisms?

<p>Energy storage and structural purposes (A)</p> Signup and view all the answers

From which precursor are nucleotides synthesized?

<p>Ribose-5-phosphate (B)</p> Signup and view all the answers

Flashcards

Microbial Nutrients

Essential elements required by microorganisms for growth and metabolism, categorized into macronutrients (e.g., carbon, nitrogen) and micronutrients (e.g., zinc, copper).

Macronutrients

Essential elements required by microorganisms in larger amounts for growth and metabolic processes.

Micronutrients

Essential elements required by microorganisms in small amounts for enzymatic functions and other biochemical activities.

Passive Diffusion

Movement of small molecules across a cell membrane without energy input, following their concentration gradient.

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Facilitated Diffusion

Movement of molecules across a cell membrane with the help of carrier proteins, following their concentration gradient.

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Active Transport

Movement of molecules across a cell membrane against their concentration gradient, requiring energy (usually ATP).

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Phototrophs

Microorganisms that use light as their primary energy source.

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Chemotrophs

Microorganisms that obtain energy from chemical compounds.

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Chemoorganotrophs

Chemotrophs that use organic compounds as their energy source.

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Chemolithotrophs

Chemotrophs that use inorganic compounds as their energy source.

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Bioenergetics

The study of energy flow through living systems, focusing on the laws of thermodynamics.

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Enzymes

Biological catalysts speeding up biochemical reactions by lowering activation energy.

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Gibbs free energy

Measures the spontaneity of biochemical reactions whether a reaction can occur on its own.

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Group Translocation

Active transport that modifies a molecule as it's moved into the cell, using energy from high-energy compounds like phosphoenolpyruvate.

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Electron Donors and Acceptors

Molecules that lose or gain electrons in redox reactions, crucial for energy production.

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Redox Reactions

Chemical reactions involving the transfer of electrons, vital for energy production in processes like respiration and photosynthesis.

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Electron Transport Chain (ETC)

A series of protein complexes that transfer electrons via redox reactions, generating a proton motive force.

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Proton Motive Force (PMF)

The electrochemical gradient formed by pumping protons across a membrane, driving ATP synthesis.

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ATP (Adenosine Triphosphate)

A high-energy compound that stores and transfers energy within cells.

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ATP Hydrolysis

The breakdown of ATP releasing energy used for cellular work (biosynthesis, transport, movement).

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Glycolysis

The breakdown of glucose into pyruvate, producing ATP and NADH.

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Fermentation

An energy conservation process in the absence of oxygen, where pyruvate is further metabolized.

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Catabolism

The breakdown of complex molecules to release energy.

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Oxidative Phosphorylation

Energy conservation using the electron transport chain and chemiosmosis

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Anaerobic respiration

A type of cellular respiration that does not require oxygen. It uses alternative electron acceptors to regenerate NAD+ and produce end products like lactic acid or ethanol, yielding less ATP compared to respiration.

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Chemolithotrophy

A metabolic process where organisms use inorganic molecules as a source of energy.

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Citric Acid Cycle

A metabolic pathway where pyruvate is oxidized, producing NADH and FADH2, which carry electrons to the electron transport chain.

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Glyoxylate Cycle

A variation of the citric acid cycle that allows organisms to convert fatty acids into carbohydrates.

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Electron Carriers (NADH, FADH2, ubiquinone)

Molecules that shuttle electrons from glycolysis and the citric acid cycle to the electron transport chain, generating a proton gradient.

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Fatty Acid Synthesis

The process of creating fatty acids from acetyl-CoA, a crucial component for cell membranes and energy storage.

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Electron Transport Chain

A series of protein complexes that use electron energy to pump protons creating a gradient.

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Study Notes

Microbial Nutrients and Nutrient Uptake

  • Microorganisms require various nutrients for growth and metabolism.
  • These nutrients are categorized into macronutrients and micronutrients.
  • Macronutrients include carbon, nitrogen, oxygen, hydrogen, phosphorus, sulfur, potassium, magnesium, and iron.
  • Micronutrients include trace elements like manganese, zinc, cobalt, molybdenum, nickel, and copper.
  • Micronutrients are vital for enzymatic functions and other biochemical activities.
  • Nutrients are transported into microbial cells via passive diffusion, facilitated diffusion, active transport, and group translocation.
  • Passive diffusion involves the movement of small molecules across the cell membrane without energy input.
  • Facilitated diffusion utilizes carrier proteins to move molecules down their concentration gradient.
  • Active transport uses energy (usually from ATP) to move substances against their concentration gradient.
  • Group translocation modifies the transported molecule using energy from high-energy compounds like phosphoenolpyruvate.

Energetics, Enzymes, and Redox

  • Microorganisms can be classified based on their energy sources: phototrophs and chemotrophs.
  • Phototrophs use light as their primary energy source.
  • Chemotrophs obtain energy from chemical compounds (chemoorganotrophs use organic compounds, chemolithotrophs use inorganic compounds).
  • Bioenergetics studies energy flow through living systems, including thermodynamics principles and Gibbs free energy (ΔG).
  • Enzymes are biological catalysts that speed up reactions by lowering activation energy.
  • Enzyme activity is often regulated by inhibitors (competitive, noncompetitive, allosteric) and modulators.
  • Redox reactions are essential for energy production, involving electron transfer from electron donors to electron acceptors.

Catabolism: Fermentation and Respiration

  • Glycolysis breaks down glucose to pyruvate, producing ATP and NADH.
  • Fermentation occurs in the absence of oxygen, regenerating NAD+ and producing end products like lactic acid or ethanol (less ATP).
  • Respiration: Citric acid or Krebs cycle oxidizes pyruvate to CO2, generating NADH and FADH2 that transfer electrons to the electron transport chain.
  • The glyoxylate cycle allows organisms to convert fatty acids into carbohydrates.
  • Electron carriers (NADH, FADH2, coenzyme Q) shuttle electrons to the electron transport chain, generating a proton gradient.
  • This proton gradient powers ATP synthesis via ATP synthase.

Biosynthesis

  • Sugars are synthesized through pathways like gluconeogenesis, converting non-carbohydrate precursors into glucose.
  • Polysaccharides (e.g., glycogen, peptidoglycan) serve for energy storage and structural purposes.
  • Amino acids are synthesized from intermediates of glycolysis, the citric acid cycle, and the pentose phosphate pathway.
  • Nucleotides are synthesized from precursors like ribose-5-phosphate and amino acids.
  • Fatty acids are synthesized from acetyl-CoA through condensation reactions, forming complex lipids (e.g., phospholipids, triglycerides).
  • These molecules are fundamental for cell structure and energy storage.

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