Microbial Metabolism and Photosynthesis
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Questions and Answers

What is the removal of electrons called?

  • Phosphorylation
  • Oxidation (correct)
  • Decarboxylation
  • Reduction

What is the gain of electrons called?

  • Glycolysis
  • Dehydrogenation
  • Reduction (correct)
  • Oxidation

What is a redox reaction?

  • A reaction that produces ATP
  • A reaction involving water
  • A reaction that breaks down carbohydrates
  • An oxidation reaction paired with a reduction reaction (correct)

In biological systems, the removal of electrons and protons at the same time is equivalent to the removal of what?

<p>Hydrogen atom (B)</p> Signup and view all the answers

What is the function of metabolic pathways of energy production?

<p>To extract energy from organic compounds and store it in ATP (B)</p> Signup and view all the answers

Which of the following is NOT a stage of carbohydrate catabolism?

<p>Calvin cycle (B)</p> Signup and view all the answers

What process does glycolysis use to break down glucose?

<p>Oxidation (B)</p> Signup and view all the answers

In aerobic respiration, what is oxidized in the Krebs cycle?

<p>Pyruvic acid (A)</p> Signup and view all the answers

Which of the following is the primary substance used to reduce CO2 in eukaryotes during photosynthesis?

<p>H atoms of H2O (C)</p> Signup and view all the answers

What type of photosynthesis is carried out by green bacteria?

<p>Anoxygenic (C)</p> Signup and view all the answers

Where does photosynthesis occur in cyanobacteria?

<p>Thylakoids (A)</p> Signup and view all the answers

Which nutritional type uses light as an energy source and organic compounds as a carbon source?

<p>Photoheterotroph (C)</p> Signup and view all the answers

Which of the following organisms is an example of a chemoautotroph?

<p>Iron-oxidizing bacteria (D)</p> Signup and view all the answers

In the electron transport chain, each NADH molecule can be oxidized to produce how many ATP molecules?

<p>3 (C)</p> Signup and view all the answers

In the electron transport chain, how many ATP molecules can each FADH2 produce?

<p>2 (D)</p> Signup and view all the answers

What is the final electron acceptor in anaerobic respiration?

<p>Not O2 (D)</p> Signup and view all the answers

Which process yields less energy: aerobic respiration or anaerobic respiration?

<p>Anaerobic respiration (C)</p> Signup and view all the answers

Which of the following is NOT a characteristic of fermentation?

<p>Requires oxygen (C)</p> Signup and view all the answers

What is the final electron acceptor in fermentation?

<p>An organic molecule (A)</p> Signup and view all the answers

Which type of fermentation produces only lactic acid?

<p>Homolactic fermentation (A)</p> Signup and view all the answers

In lactic acid fermentation, what is glucose oxidized to?

<p>Pyruvic acid (A)</p> Signup and view all the answers

What products are produced during alcohol fermentation?

<p>Ethanol + CO2 (C)</p> Signup and view all the answers

In alcohol fermentation, what is pyruvic acid converted to?

<p>Acetaldehyde and CO2 (B)</p> Signup and view all the answers

What process do bacteria use to catabolize carbohydrate or protein in a fermentation test?

<p>Produce acid (B)</p> Signup and view all the answers

What does the oxidase test identify in bacteria?

<p>Cytochrome oxidase (A)</p> Signup and view all the answers

What is light energy converted into during the light-dependent reactions of photosynthesis?

<p>Chemical energy (A)</p> Signup and view all the answers

What two products of the light-dependent reactions are used to reduce $CO_2$ to sugar in the Calvin-Benson cycle?

<p>ATP and NADPH (B)</p> Signup and view all the answers

In oxygenic photosynthesis, what gas is produced?

<p>Oxygen (B)</p> Signup and view all the answers

What type of organism uses light energy?

<p>Phototrophs (D)</p> Signup and view all the answers

What do photoautotrophs use as an energy source in the Calvin-Benson cycle?

<p>Light (D)</p> Signup and view all the answers

Which process precedes the Krebs cycle in protein catabolism?

<p>Deamination (D)</p> Signup and view all the answers

What is the initial step in protein catabolism?

<p>Hydrolysis by extracellular proteases (C)</p> Signup and view all the answers

What is produced in anoxygenic photosynthesis?

<p>Sulfur (D)</p> Signup and view all the answers

Which microorganism is used in the production of beer and wine?

<p>Saccharomyces cerevisiae (C)</p> Signup and view all the answers

What is the starting material for vinegar production?

<p>Ethanol (B)</p> Signup and view all the answers

Which of the following is created using Lactobacillus and Streptococcus?

<p>Yogurt (C)</p> Signup and view all the answers

What are the end-products in the making of Swiss cheese?

<p>Propionic Acid and Carbon Dioxide (B)</p> Signup and view all the answers

Which of the end products below are used in pharmaceutical and industrial applications?

<p>Acetone and Butanol (D)</p> Signup and view all the answers

What microorganism is used to create vitamin C?

<p>Gluconobacter (C)</p> Signup and view all the answers

What material is fermented by Lactobacillus plantarum to produce sauerkraut?

<p>Cabbage (B)</p> Signup and view all the answers

What is the role of Methanosarcina?

<p>Fuel production (D)</p> Signup and view all the answers

Which of the following uses lactic acid as a starting material?

<p>Swiss cheese (A)</p> Signup and view all the answers

Which of the following microorganisms is a fungus?

<p>Aspergillus (A)</p> Signup and view all the answers

What structure within eukaryotes is the site of photosynthesis?

<p>Chloroplasts (B)</p> Signup and view all the answers

What type of chlorophyll is present in green bacteria?

<p>Bacteriochlorophyll a (A)</p> Signup and view all the answers

What substance reduces $CO_2$ in green bacteria?

<p>Sulfur (D)</p> Signup and view all the answers

Which of the following is an example of a photoheterotroph?

<p>Green bacteria (C)</p> Signup and view all the answers

In what environment do purple bacteria carry out photosynthesis?

<p>Anaerobic (A)</p> Signup and view all the answers

Which microorganism is used in beer production?

<p>Saccharomyces cerevisiae (D)</p> Signup and view all the answers

What is the starting material for the production of yogurt?

<p>Milk (C)</p> Signup and view all the answers

Which of the following end-products are used in the production of Swiss cheese?

<p>Propionic Acid and Carbon Dioxide (A)</p> Signup and view all the answers

Which microorganism is used in the production of rye bread?

<p>Lactobacillus delbrueckii (C)</p> Signup and view all the answers

In the electron transport chain, what is the maximum number of ATP molecules that can be produced from one NADH molecule?

<p>3 (C)</p> Signup and view all the answers

In anaerobic respiration, what molecule is NOT the final electron acceptor in the electron transport chain?

<p>Oxygen (A)</p> Signup and view all the answers

Which process yields less energy?

<p>Anaerobic respiration (A)</p> Signup and view all the answers

In both lactic acid and alcohol fermentation, what is glucose oxidized to?

<p>Pyruvic acid (B)</p> Signup and view all the answers

What is used as the final electron acceptor in fermentation?

<p>An organic molecule (B)</p> Signup and view all the answers

What process is defined as the removal of electrons?

<p>Oxidation (C)</p> Signup and view all the answers

In biological systems, what is the removal of both electrons and protons equivalent to?

<p>Removal of a hydrogen atom (C)</p> Signup and view all the answers

What product is formed from the oxidation of glucose during glycolysis?

<p>Pyruvic acid (B)</p> Signup and view all the answers

What is the main purpose of metabolic pathways in energy production?

<p>To extract energy from organic compounds and store it as ATP (C)</p> Signup and view all the answers

What is released during the oxidation of acetyl CoA in the Krebs cycle?

<p>Carbon Dioxide (C)</p> Signup and view all the answers

In aerobic respiration, where does the final electron acceptor originate?

<p>From outside the cell and is inorganic (D)</p> Signup and view all the answers

What do bacteria that catabolize carbohydrates or proteins produce in a fermentation test?

<p>Acid (D)</p> Signup and view all the answers

What is produced during the light-dependent reactions of photosynthesis?

<p>ATP and NADPH (D)</p> Signup and view all the answers

Besides ATP, what other molecules are produced from the oxidation of glucose during glycolysis?

<p>NADH (A)</p> Signup and view all the answers

What process occurs when pyruvic acid is converted to Acetyl CoA?

<p>Decarboxylation (D)</p> Signup and view all the answers

What is the purpose of the Calvin-Benson cycle?

<p>Reduction of CO2 to sugar (D)</p> Signup and view all the answers

Which of the following is a characteristic of oxygenic photosynthesis?

<p>Production of oxygen (C)</p> Signup and view all the answers

What energy source do phototrophs utilize?

<p>Light (D)</p> Signup and view all the answers

What is the function of extracellular proteases?

<p>Break down proteins into amino acids. (A)</p> Signup and view all the answers

What do photoautotrophs use to fix $CO_2$ to sugar?

<p>Light energy (C)</p> Signup and view all the answers

What is produced in anoxygenic photosynthesis instead of oxygen?

<p>Sulfur (D)</p> Signup and view all the answers

The oxidase test identifies bacteria that have which enzyme?

<p>Cytochrome oxidase (D)</p> Signup and view all the answers

What is the overall purpose of light-dependent reactions?

<p>To convert light energy into chemical energy (C)</p> Signup and view all the answers

Flashcards

Oxidation

The removal of electrons from a substance.

Reduction

The gain of electrons by a substance.

Redox Reaction

A paired oxidation and reduction reaction.

Metabolic Pathways

Series of chemical reactions that extract energy from organic molecules and store it as ATP.

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Carbohydrate Catabolism

The breakdown of carbohydrates to release energy.

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Glycolysis

The oxidation of glucose to pyruvic acid, producing ATP and NADH.

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Cellular Respiration

Uses an electron transport chain; final electron acceptor is inorganic and from outside the cell; ATP generated through oxidative phosphorylation.

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Krebs Cycle

Pyruvic acid is oxidized and decarboxylation occurs, producing NADH, FADH2, ATP, and CO2.

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NADH ATP Yield

Each NADH molecule yields 3 ATP molecules in the electron transport chain.

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FADH2 ATP Yield

Each FADH2 molecule yields 2 ATP molecules in the electron transport chain.

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Anaerobic Respiration

A type of respiration where the final electron acceptor in the electron transport chain is NOT oxygen.

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Fermentation

Metabolic process that releases energy from oxidation of organic molecules, does not require oxygen, Krebs or ETC, and uses an organic molecule as final electron acceptor.

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Lactic Acid Fermentation

A type of fermentation that produces lactic acid.

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Homolactic Fermentation

Fermentation that produces only lactic acid.

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Heterolactic Fermentation

Fermentation that produces lactic acid and other compounds.

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Lactic Acid Fermentation Process

Glucose is oxidized to pyruvic acid, then reduced by NADH during lactic acid fermentation.

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Alcohol Fermentation

A type of fermentation that produces ethanol and CO2.

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Photoautotroph

Uses light as an energy source and CO2 as a carbon source.

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Photoheterotroph

Uses light as an energy source and organic compounds as a carbon source.

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Chemoautotroph

Uses inorganic chemicals as an energy source and CO2 as a carbon source.

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Chemoheterotroph

Uses chemicals as an energy source and organic compounds as a carbon source.

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Anoxygenic Photosynthesis

Photosynthesis that does NOT produce oxygen. Uses molecules other than water (H2O = Oxygenic) for e-

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Ethanol

A fermentation end-product used in beer and wine production.

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Acetic Acid

A fermentation end-product that gives vinegar its sour taste.

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Lactic Acid

A fermentation end-product found in cheese and yogurt.

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Propionic Acid and Carbon Dioxide

End product used in manufacturing Swiss cheese.

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Acetone and Butanol

Fermentation end-products with pharmaceutical and industrial applications.

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Citric Acid

A flavoring produced by fermentation. Also used as a preservative.

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Methane

A fuel produced by fermentation, especially from acetic acid.

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Saccharomyces cerevisiae

The microorganism used to produce ethanol for beer and wine.

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Acetobacter

The microorganism responsible for the sour taste of wine.

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Propionibacterium freudenreichii

The microorganism used in Swiss cheese production.

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Extracellular Proteases

Breaks down proteins outside the cell.

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Deamination

The process of removing an amino group from an amino acid.

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Decarboxylation

The process of removing a carboxyl group (COOH) from an organic molecule, releasing carbon dioxide (CO2).

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Fermentation Test

Bacteria catabolize carbohydrate or protein, producing acid and changing the pH indicator color.

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Oxidase Test

Identifies bacteria with cytochrome oxidase.

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Light-Dependent Reactions

Light energy is converted into chemical energy (ATP and NADPH).

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Light-Independent Reactions

ATP and NADPH are used to reduce CO2 to sugar (carbon fixation) via the Calvin-Benson cycle.

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Phototrophs

Organisms that use light energy.

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Oxygenic Photoautotroph

Uses light as an energy source and CO2 as a carbon source, producing oxygen.

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Anoxygenic Photoautotroph

Uses light as an energy source and CO2 as a carbon source, but does not produce oxygen.

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Fermentation Definition

Releases energy from organic molecules, doesn't require oxygen, Krebs cycle, or ETC; uses organic molecule as final electron acceptor; produces small ATP amounts.

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Ethanol Fermentation (Beer/Wine)

Production of beer and wine via fermentation.

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Ethanol Fermentation (Fuel)

Ethanol production from agricultural waste.

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Lactic Acid Fermentation (Dairy)

Production of cheese and yogurt through fermentation.

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Sorbose

A fermentation end-product used to make Vitamin C.

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Industrial Fermentation

Industrial process using bacteria or archaea to convert organic materials into valuable chemicals.

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Lactobacillus

A bacterium used in lactic acid production.

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Citric Acid Fermentation

Used to create the flavor in some foods.

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Methane Fermentation

Fuel produced from organic waste by archaea.

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Fermentative Microorganism

Microorganism that carries out a specific fermentation process.

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Lipid Catabolism

Breaks down lipids.

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Photoautotrophs (Oxygenic)

Use light and CO2 to produce sugars; produce O2.

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Photoautotrophs (Anoxygenic)

Use light and CO2 to produce sugars; do NOT produce O2.

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Study Notes

Oxidation-Reduction Reactions

  • Oxidation refers to the removal of electrons from a substance.
  • Reduction signifies the gain of electrons by a substance.
  • Oxidation and reduction reactions often occur in tandem, forming what is known as a redox reaction.
  • In biological systems, the removal of electrons is often accompanied by protons, effectively removing a hydrogen atom.
  • Dehydrogenation reactions are common types of biological oxidations.

Metabolic Pathways of Energy Production

  • Metabolic pathways consist of a sequence of chemical reactions, each facilitated by an enzyme.
  • The purpose of these pathways is to extract energy from organic compounds.
  • The energy extracted is then stored in the form of ATP (adenosine triphosphate), the cell's energy currency.

Carbohydrate Catabolism

  • Carbohydrate catabolism involves the enzymatic breakdown of carbohydrates to liberate energy.
  • Glycolysis, a fundamental pathway, participates in the breaking down of carbohydrates.
  • The Krebs cycle is a vital stage in carbohydrate catabolism, further processing the products of glycolysis.
  • The electron transport chain is a system is the final stage, where much of the energy is captured as ATP.

Glycolysis

  • Glycolysis is the oxidation of glucose into pyruvic acid.
  • The oxidation of the glucose yields ATP, usable energy for the cell.
  • NADH, an electron carrier used to generate more ATP, also gets produced

Cellular Respiration

  • The oxidation of molecules releases electrons, which are then used to power an electron transport chain.
  • Comes from outside of the cell, the final electron acceptor is inorganic.
  • Oxidative phosphorylation directly generates ATP.

Aerobic Respiration

  • The Krebs cycle is a key component of aerobic respiration.
  • Pyruvic acid from glycolysis gets oxidized during the preparatory step.
  • Decarboxylation, the loss of CO2, occurs during the preparatory step.
  • The resulting two-carbon compound binds to coenzyme A.
  • The two-carbon compound attaching to coenzyme A forms acetyl CoA and NADH.
  • Oxidation of acetyl CoA inside the cycle leads to the production of NADH, FADH2, and ATP.
  • Oxidation of acetyl CoA also liberates CO2 as waste

Carbohydrate Catabolism - ATP Production

  • 3 molecules of ATP produce from, each NADH when oxidized in the electron transport chain.
  • 2 molecules of ATP produce from, each FADH2.

Anaerobic Respiration

  • The final electron acceptor in the electron transport chain is not O2, setting it apart from aerobic respiration.
  • From aerobic respiration, anaerobic respiration yields less energy.
  • Nitrate (NO3-) is used in during process, producing NO2-, N2 + H2O.
  • (SO4-) is used in during process, producing H2S + H2O.
  • Carbonate (CO32-) is used in during process, producing CH4 + H2O.

ATP Yield During Prokaryotic Aerobic Respiration

  • Glycolysis oxidation of glucose to pyruvic acid produces 2 ATP via substrate-level phosphorylation.
  • Glycolysis production of 2 NADH produces 6 ATP via oxidative phosphorylation in the electron transport chain.
  • The preparatory step formation of acetyl CoA produces 2 NADH which generate 6 ATP, via oxidative phosphorylation in the electron transport chain.
  • In the Krebs cycle, oxidation of succinyl CoA to succinic acid yields 2 GTP equivalent to ATP via substrate-level phosphorylation.
  • The Krebs cycle production of 6 NADH produces 18 ATP via oxidative phosphorylation in the electron transport chain
  • The Krebs cycle production of 2 FADH produces 4 ATP via oxidative phosphorylation in the electron transport chain.
  • 38 ATP is Total ATP yield during prokaryotic aerobic respiration of one glucose molecule is.

Fermentation

  • Fermentation extracts energy from organic molecules through oxidation.
  • Fermentation takes place without the need for oxygen.
  • Fermentation does not use the Krebs cycle or the electron transport chain.
  • An organic molecule serves as the final electron acceptor in fermentation.
  • Fermentation produces only small amounts of ATP, in contrast to respiration.
  • Lactic acid fermentation results in the production of lactic acid.
    • Homolactic fermentation exclusively produces lactic acid.
    • Heterolactic fermentation yields lactic acid along with other compounds.
  • In lactic acid fermentation, glucose is initially oxidized to pyruvic acid, and then reduced by NADH.
  • Alcohol fermentation results in the production of ethanol and CO2.
  • First, glucose is oxidized to pyruvic acid.
  • Then pyruvic acid is converted to acetaldehyde and CO2.
  • Finally, NADH reduces acetaldehyde to ethanol.

Industrial Uses of Fermentation

  • Ethanol is for beer and wine and starts with starch or sugar.
    • Ethanol uses Saccharomyces cerevisiae.
  • Ethanol is for fuel, starting with agricultural waste.
    • Ethanol uses Saccharomyces cerevisiae.
  • Acetic Acid is for Vinegar
    • Acetic Acid uses Acetobacter, starting with Ethanol.
  • Lactic Acid is for cheese and yogurt
    • Lactic Acid uses Lactobacillus, Streptococcus starting with Milk.
  • Lactic Acid is for rye bread
    • Lactic Acid uses Lactobacillus delbrueckii, starting with grain and sugar.
  • Lactic Acid is for sauerkraut
    • Lactic Acid uses Lactobacillus plantarum, starting with cabbage.
  • Lactic Acid is for summer sausage
    • Lactic Acid uses Pediococcus, starting with meat.
  • Propionic Acid and Carbon Dioxide is for swiss cheese
    • Propionic Acid and Carbon Dioxide uses Propionibacterium freudenreichii.
  • Acetone and Butanol is for pharmaceutical and industrial purposes
    • Acetone and Butanol uses Clostridium acetobutylicum, starting with molasses.
  • Citric Acid is used as a flavoring
    • Citric Acid uses Aspergillus (fungus), starting with molasses.
  • Methane is for fuel
    • Methane uses Methanosarcina (archaeon), starting with acetic acid.
  • Sorbose is for Vitamin C (ascorbic acid)
    • Sorbose uses Gluconobacter, starting with sorbitol.
  • The microorganisms are typically bacteria unless otherwise specified.

Lipid and Protein Catabolism

  • Lipid catabolism: Using Lipase Lipids are converted to glycerol and fatty acids,
    • Glycerol becomes Dihydroxyacetone phosphate, becomes Glyceraldehyde 3-phosphate, becomes Pyruvic acid, becomes Acetyl CoA.
  • Protein catabolism: The Deamination, decarboxylation, dehydrogenation, and desulfurization of amino acids fuel the Krebs Cycle. -Protein becomes Amino acids and these alterations turn them into an organic acid.

Biochemical Tests

  • The fermentation test distinguishes bacteria based on their ability to catabolize carbohydrates or proteins, producing acid and causing a color change in a pH indicator.
  • The oxidase test identifies bacteria with cytochrome oxidase, exemplified by Pseudomonas.

Photosynthesis

  • Light-dependent (light) reactions capture light energy.
    • Conversion of light energy into chemical energy such as ATP and NADPH.
  • Light-independent (dark) reactions, ATP and NADPH reduce CO2.
    • The Calvin-Benson cycle drives the reduction of CO2, resulting to sugar and carbon fixation.

Photosynthesis Chemical Equation

  • In oxygenic photosynthesis, 6CO2 + 12H2O + Light energy becomes C6H12O6 + 6H2O + 6O2.
  • In anoxygenic photosynthesis, 6CO2 + 12H2S becomes C6H12O6 + 6H2O + 12 S under light energy.

Metabolic Diversity Among Organisms

  • Phototrophs use light energy to power their metabolism.
  • Photoautotrophs fix CO2 to generate sugars using the Calvin-Benson cycle.
    • Oxygenic organisms release O2 during photosynthesis.
    • Anoxygenic organisms do not produce O2.

Photosynthesis in Selected Eukaryotes and Prokaryotes

  • Eukaryotes, use H atoms of H2O to reduce CO2 , produce Oxygen, and Chlorophyll a.
    • Eukaryotes use Chloroplasts with thylakoids for Photosynthesis site, and have Aerobic environments.
  • Prokaryotes Cyanobacteria reduce H atoms of HOO with CO2 , produce Oxygen and Chlorophyll a.
    • Thylakoids are the Photosynthesis site, and Aerobic(and anaerobic) environments for Prokaryotes Cyanobacteria
  • Prokaryotes Green Bacteria use Sulfur, sulfur compounds or H2 gas to reduce CO2, Anoxygenic and Bacteriochlorophyll a.
    • Prokaryotes Green use Chlorosomes Photosynthesis site, and Anaerobic environments,
  • Sulfur, Prokkarytoes Purple Bacteria use sulfur compounds or H2 gas to reduce CO2, are Anoxygenic and Bacteriochlorophyll a or b.
    • Prokarytoes Purple use Chromatophores Photosynthesis site, and Anaerobic environments.

Metabolic Diversity Nutritional Type

  • Photoautotrophs harness light for energy and CO2 for their carbon source.
    • Notable examples include oxygenic Cyanobacteria and plants, along with anoxygenic green and purple bacteria.
  • Photoheterotrophs utilize light energy.
    • Photoheterotrophs consume organic compounds as their carbon source.
    • Instances of organisms are green and purple non-sulfur bacteria.
  • Chemoautotrophs derive energy from inorganic chemicals.
    • Chemoautotrophs fix CO2 as their carbon source.
    • A prime example is iron-oxidizing bacteria.
  • Chemoheterotrophs obtain their energy from chemicals and carbon from organic compounds.
    • This group includes fermentative bacteria, animals, protozoa, and fungi.

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Description

This quiz covers fundamental concepts in microbial metabolism, including oxidation-reduction reactions, carbohydrate catabolism, and photosynthesis. It explores energy production pathways, electron transport chains, and nutritional types of microorganisms. Questions address key metabolic processes in bacteria and eukaryotes.

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