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Questions and Answers

What is the main driver of the evolutionary process?

  • Deletions
  • Recombination
  • Mutations (correct)
  • Insertions
  • What type of mutations can be classified as requiring short flanking segments of similar sequence?

  • Insertions
  • Deletions
  • Recombination
  • Substitutions (correct)
  • Which type of mutations does not require high similarity in the DNA sequence?

  • Deletions
  • Duplications
  • Nonhomologous (correct)
  • Insertions
  • What is defined by the ability of an organism to produce progeny and contribute to the genetic makeup of future generations?

    <p>Fitness</p> Signup and view all the answers

    What is the objective of the polyphasic approach to taxonomy?

    <p>To reach a consensus classification by integrating multiple forms of data</p> Signup and view all the answers

    What does the biological species concept propose?

    <p>A species is an interbreeding population that is reproductively isolated from other such populations</p> Signup and view all the answers

    What is the problematic aspect of the biological species concept when describing bacteria and archaea?

    <p>Reproductive isolation is difficult to apply to microorganisms</p> Signup and view all the answers

    What defines a microbial species?

    <p>Monophyletic, genomically coherent, phenotypically coherent, and distinguishable from other species</p> Signup and view all the answers

    What is the most commonly used metric for estimating overall relatedness based on genome analysis?

    <p>Average nucleotide identity</p> Signup and view all the answers

    What distinguishes multilocus sequence analysis from rRNA gene sequencing for taxonomic assignment?

    <p>It uses protein-encoding genes and can distinguish species that rRNA sequences cannot resolve</p> Signup and view all the answers

    What are the three methods used in the polyphasic approach to taxonomy?

    <p>Phenotypic, genotypic, and phylogenetic analysis</p> Signup and view all the answers

    What is the significance of SSU (small subunit) ribosomal RNA (rRNA) genes in molecular phylogeny?

    <p>They are highly conserved and easily sequenced and analyzed</p> Signup and view all the answers

    What is the criterion for considering strains to belong to the same species based on SSU rRNA gene sequencing?

    <p>Exhibiting &gt;97% SSU rRNA sequence identity</p> Signup and view all the answers

    What characteristic distinguishes the polyphasic approach to taxonomy from other methods?

    <p>Integration of multiple forms of data and information into a consensus classification</p> Signup and view all the answers

    What is the primary focus of phenotypic analysis in taxonomy?

    <p>Morphological, metabolic, physiological, and chemical characteristics</p> Signup and view all the answers

    What can be used as a molecular clock to estimate time since species diverged?

    <p>Sequence changes</p> Signup and view all the answers

    Which process can cause gene frequencies to change over time, particularly in small populations or those experiencing bottleneck events?

    <p>Genetic drift</p> Signup and view all the answers

    What allows one copy of a gene to evolve new function while the other continues with the original function?

    <p>Gene duplication</p> Signup and view all the answers

    Which type of mutation allows citrate utilization in E. coli after a dramatic increase in fitness over 60,000 generations?

    <p>Gain-of-function mutation</p> Signup and view all the answers

    What plays a crucial role in microbial genome dynamics, occurring more often than insertions/duplications and maintaining small genome size?

    <p>Gene deletions</p> Signup and view all the answers

    Which process allows DNA transfer between distant branches of the evolutionary tree, impacting microbial evolution through mechanisms like transformation, transduction, and conjugation?

    <p>Horizontal gene transfer</p> Signup and view all the answers

    What can be used to reveal diversity within species, with microbial genomes being highly diverse and dynamic, consisting of individual strains differing in composition?

    <p>Comparative genomics</p> Signup and view all the answers

    What type of mutations allows the observation of loss-of-function and rare gain-of-function mutations in experimental evolution with microbes?

    <p>Loss-of-function and rare gain-of-function mutations</p> Signup and view all the answers

    What did the long-term experimental evolution in E. coli over 60,000 generations demonstrate?

    <p>A dramatic increase in fitness</p> Signup and view all the answers

    What did the selection in a population of phototrophic purple bacteria, Rhodobacter capsulatus, show?

    <p>Random mutations can lead to fitness changes</p> Signup and view all the answers

    What did genome analysis suggest about Saccharomyces cerevisiae?

    <p>Whole genome duplication</p> Signup and view all the answers

    What type of analysis impacts the prediction of protein function in different organisms through gene families such as homologs, orthologs, and paralogs?

    <p>Comparative genomics</p> Signup and view all the answers

    Study Notes

    Microbial Evolution and Genetic Dynamics

    • Microbial evolution involves mutations occurring by chance, with the environment selecting advantageous mutations.
    • Genetic drift, a random process, can cause gene frequencies to change over time, particularly in small populations or those experiencing bottleneck events.
    • Species can encompass various strains with different traits, and sequence changes can be used as a molecular clock to estimate time since species diverged.
    • Experimental evolution with microbes allows the observation of evolutionary events, such as loss-of-function and rare gain-of-function mutations.
    • Selection in a population of phototrophic purple bacteria, Rhodobacter capsulatus, showed how random mutations can lead to fitness changes.
    • Long-term experimental evolution in E. coli over 60,000 generations demonstrated a dramatic increase in fitness, followed by a gain-of-function mutation allowing citrate utilization.
    • Gene duplication allows one copy to evolve new function while the other continues with the original function, and genome analysis suggests whole genome duplication in Saccharomyces cerevisiae.
    • Genome evolution involves gene families, such as homologs, orthologs, and paralogs, impacting the prediction of protein function in different organisms.
    • Gene deletions play a crucial role in microbial genome dynamics, occurring more often than insertions/duplications and maintaining small genome size.
    • Deletions can drive interdependence in microbial communities and are essential for obligate intracellular symbionts and intracellular pathogens.
    • Horizontal gene transfer allows DNA transfer between distant branches of the evolutionary tree, impacting microbial evolution through mechanisms like transformation, transduction, and conjugation.
    • Comparative genomics reveals diversity within species, with microbial genomes being highly diverse and dynamic, consisting of individual strains differing in composition.

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    Test your knowledge of microbial evolution and genetic dynamics with this quiz. Explore topics such as mutations, genetic drift, experimental evolution, gene duplication, genome evolution, gene deletions, and horizontal gene transfer in microbial communities.

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