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Questions and Answers
What is the meaning of the term 'symbiosis'?
What is the meaning of the term 'symbiosis'?
Symbiosis, or 'life in common,' describes various interactions between microorganisms and between microorganisms and higher organisms like plants and animals. These interactions can be positive or negative.
Define the field of microbial ecology.
Define the field of microbial ecology.
Microbial ecology is the study of the interactions between microorganisms and their environment, including interactions between different microorganisms and between microorganisms and higher organisms (animals and plants).
Distinguish between microbial ecology and environmental microbiology.
Distinguish between microbial ecology and environmental microbiology.
Microbial ecology focuses on the interactions between microorganisms and their immediate environments, while environmental microbiology examines larger microbial processes occurring in broader environments like water, soil, and food.
What are the major types of positive interactions found in microbial communities?
What are the major types of positive interactions found in microbial communities?
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List the main types of negative interactions between microorganisms.
List the main types of negative interactions between microorganisms.
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How do microbial interactions affect natural processes?
How do microbial interactions affect natural processes?
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What is the impact of microbial interactions on disease onset?
What is the impact of microbial interactions on disease onset?
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Explain how microbial interactions can be influenced by environmental changes.
Explain how microbial interactions can be influenced by environmental changes.
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What is the key difference between genomics and metagenomics?
What is the key difference between genomics and metagenomics?
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How does metatranscriptomics help us understand the functional profile of a microbial community?
How does metatranscriptomics help us understand the functional profile of a microbial community?
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Describe the primary focus of metabolomics in the study of microbial communities.
Describe the primary focus of metabolomics in the study of microbial communities.
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Why is the metabolome considered a direct indicator of environmental health?
Why is the metabolome considered a direct indicator of environmental health?
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Explain why metabolomics is a valuable tool for pathway analysis.
Explain why metabolomics is a valuable tool for pathway analysis.
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What are the limitations of omics techniques in understanding microbial communities?
What are the limitations of omics techniques in understanding microbial communities?
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Why is it crucial to study the dynamics of community composition in relation to environmental parameters?
Why is it crucial to study the dynamics of community composition in relation to environmental parameters?
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Explain the significance of identifying predictable patterns in microbial community dynamics.
Explain the significance of identifying predictable patterns in microbial community dynamics.
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Describe the role of microorganisms in the formation of biofilms.
Describe the role of microorganisms in the formation of biofilms.
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Explain the concept of 'quorum sensing' and its impact on microorganisms.
Explain the concept of 'quorum sensing' and its impact on microorganisms.
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What are the fundamental requirements that microorganisms need to thrive in their environment?
What are the fundamental requirements that microorganisms need to thrive in their environment?
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How do microorganisms contribute to biogeochemical recycling?
How do microorganisms contribute to biogeochemical recycling?
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Explain the importance of microorganisms in ecosystem succession.
Explain the importance of microorganisms in ecosystem succession.
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What are the three major domains of life, and give an example of each.
What are the three major domains of life, and give an example of each.
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Describe the two main categories of bacteria based on their habitat.
Describe the two main categories of bacteria based on their habitat.
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What are the essential elements that organisms need to survive?
What are the essential elements that organisms need to survive?
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Flashcards
Microbiome
Microbiome
A microbial community in a defined habitat.
Metagenomics
Metagenomics
Study of the genetic material of entire microbial communities.
Next-generation sequencing (NGS)
Next-generation sequencing (NGS)
A method for rapidly sequencing DNA after extraction from samples.
Metatranscriptomics
Metatranscriptomics
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Metatranscriptome
Metatranscriptome
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Metabolomics
Metabolomics
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Metabolome
Metabolome
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Community Dynamics
Community Dynamics
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Biofilms
Biofilms
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Quorum Sensing
Quorum Sensing
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Microbial Energy Needs
Microbial Energy Needs
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Biogeochemical Recycling
Biogeochemical Recycling
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Carbon Cycle
Carbon Cycle
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Prokaryotes
Prokaryotes
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Eukaryotes
Eukaryotes
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Ecosystem Succession
Ecosystem Succession
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Metatranscriptome analysis
Metatranscriptome analysis
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Orbicella faveolata
Orbicella faveolata
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Holobiont
Holobiont
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Symbiosis
Symbiosis
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Microbial ecology
Microbial ecology
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Environmental microbiology
Environmental microbiology
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Positive interactions
Positive interactions
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Negative interactions
Negative interactions
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Study Notes
Marine Microbial Ecology - Introduction
- This is an introductory chapter to marine microbial ecology.
- The course is prepared by Dr. Claude DAOU, Faculty of Science - LU.
- The subject matter of the study will include microbial foundations, interactions, nutrient cycles, the physical environment, and microorganism roles in aquatic environments.
Plan
- The course covers fundamental microbial ecology.
- Microbial interactions include mutualism, commensalism, parasitism, and symbiosis.
- The study of nutrient cycles will encompass those of carbon, sulfur, and nitrogen.
- The physical environment, including microhabitats, biofilms, ecosystem interactions, and environmental stress, will be discussed.
- Microorganisms in aquatic environments will be explored.
Concepts (page 3)
- Marine microbial communities (bacteria, archaea, protists, fungi, viruses) are responsible for half of the global biogeochemical flux of biologically important elements (carbon, nitrogen, phosphorus, sulfur, iron).
- These organisms are either phototrophic or chemotrophic primary producers, as well as heterotrophic secondary producers that recycle organic carbon and nutrients in the microbial loop.
Concepts (page 4)
- Molecular analysis plays a crucial role in determining the organisms present in a given location and their distribution.
- Phylogenetic analyses using genes like 16S and 18S rRNA genes are used, though sole phylogenetic identification is insufficient for assessing the environmental functions and ecology.
- Metagenomics, metatranscriptomics, and metabolomics analyses provide further information.
Concepts (page 5)
- Metagenomics, metatranscriptomics, and metabolomics provide detailed information about microbial communities and their functions.
- These analyses alone are insufficient for understanding interactions, competition, symbiosis between organisms, and their overall roles in the marine environment.
Concepts (page 6)
- A microbiome is defined as a microbial community occupying a well-defined habitat.
- Metagenomics, the analysis of genetic material from entire communities of organisms, is commonly used to study microbiomes.
- This typically involves next-generation sequencing (NGS) after DNA extraction from samples.
Concepts (page 7)
- Metatranscriptomics focuses on expressed genes to understand the active functional profile of microbial communities.
- It captures the total mRNA present at a particular time and under specific conditions.
Concepts (page 8)
- Metabolomics is a comprehensive analysis quantifying all metabolites (small molecules released by organisms into the environment).
- The metabolome is a direct indicator of environmental health and alterations in homeostasis.
Concepts (page 9, 10)
- Additional insights beyond omics are crucial for predicting community functions and interactions between organisms.
- Analyzing community composition and environmental parameters can reveal predictable trends.
- The term "dynamics" refers to changes in population abundance within a community.
- "Community" refers to the composition of microorganisms and their relative proportions.
Concepts (page 11)
- Marine microbial communities constantly fluctuate yet exhibit predictable behaviors across daily, seasonal, and interannual variations in composition.
- Internal feedback mechanisms (competition, viral infections, and predator-prey interactions) play a role in maintaining consistent community composition.
Concepts (page 12, 13)
- Microbial changes occur across multiple timescales in response to various biological and non-biological environmental factors.
- Copiotrophic organisms (Gammaproteobacteria, Flavobacteria, Alphaproteobacteria) are frequently observed; these organisms can rapidly become abundant under suitable conditions (e.g., timescales of hours).
Concepts (page 14, 15)
- Whole-community biomass turnover times (from less than a day to a week) in the offshore surface ocean provide a timescale for observing microbial responses.
- These responses to environmental variations include weathering, meso-scale oceanographic processes, interactions with larger organisms, food webs, and microbial interactions among viruses, bacteria, archaea, and protists.
- Other dynamic factors associated with daily to weekly timescales include phytoplankton blooms, cross-feeding interactions, toxins/allelopathic substances, oxygen depletion, and large organism mortality.
Concepts (page 16)
- Factors influencing community composition over monthly to seasonal timescales include variations in solar angle/intensity, storm frequencies, upwelling patterns, nutrient availability, and stratification.
- Changes in temperature, day length, land runoff, atmospheric deposition, and interactions with larger organisms and microbial species also affect composition.
Concepts (page 17, 18)
- Long-term time series observations provide a straightforward approach for understanding marine microbial community dynamics.
- Major time-series sites are accompanied by numerous smaller-scale investigations typically focused on determining physical/chemical oceanographic variables.
- White Plague disease (WPD) is implicated in coral reef decline in the Caribbean, affecting microbial communities in coral mucus and tissue.
- Metatranscriptomic analysis of corals (Orbicella faveolata) indicates holobiont response to disease and various inter-related gene expression changes in the host coral, algae, and microbial compartments involved.
Concepts (page 19, 20)
- Corals, algal symbionts, and microbial compartments exhibit both holobiont-wide and compartment-specific responses to WPD.
- Analyses reveal various differentially expressed genes in each compartment, associated with processes like innate immunity, stress response, and metabolism.
Concepts (page 21, 22)
- Significant differentially-expressed genes or abundances of bacterial lineages (e.g., Gammaproteobacteria, Flavobacteria, Rhodobacter spp.) are identified as involved in coral disease.
- Bacterial family distributions are displayed with high mRNA sequence abundance for each function/process during this coral disease stage.
Concepts (page 23, 24)
- Symbiosis, or "life in common," is defined as numerous interactions between microorganisms and also includes interactions with higher organisms.
- These interactions can be positive or negative.
- The science of microbial ecology studies the interactions between microorganisms and their environment and also includes interactions between micro-organisms themselves and higher organisms like animals and plants.
Concepts (page 25)
- A diagram shows the interrelationships between marine microbial ecology and other scientific disciplines (e.g., oceanography, limnology, pedology, chemistry, medicine, and agronomy); this indicates the interdisciplinary nature of microbial ecology.
Concepts (page 26)
- The hierarchical levels of organization include individuals, populations, guilds (communities), ecosystems, and habitats with illustrative figures.
- Defining characteristics of different levels are highlighted with specific definitions.
Concepts (page 27)
- Microbial ecology studies the behavior and activities of microorganisms within their micro-environments; environmental microbiology focuses on global microbial processes.
Concepts (page 28)
- Microbial interactions can be positive (mutualism, protocooperation, commensalism) or negative (parasitism, predation, amensalism, competition).
- These interactions vary based on the environment and involved organisms.
Concepts (page 29)
- Microbial interactions are categorized into those between micro-organisms, plants, and animals.
- Symbiosis is defined as cooperative mutualistic relationships (mutualism, commensalism, amensalism).
Concepts (page 30)
- Microorganisms can form complex physical structures (biofilms) that impact microbial survival and disease outbreaks.
- Microorganisms interact using chemical signals that control various microbial properties via quorum perception.
Concepts (page 31)
- Suitable physical environments for microbial life require the availability of energy, electrons, and nutrients.
- Microorganisms interact with their environment to obtain these resources.
- Microorganism alter the chemical and physical states of nutrients to use them in growth processes.
Concepts (page 32)
- The carbon cycle illustrates the interconnectedness of microbial, animal, and plant life.
- Various processes, like fermentation and respiration, are essential parts of this cycle.
Concepts (page 33)
- Microorganisms are key components of ecosystems.
- They influence ecosystem succession.
- The study of microbial interactions employs techniques like microscopy, chemical, enzymatic, and molecular analyses.
Concepts (page 34)
- Ecosystems display high spatial and temporal heterogeneity.
- Diverse habitats, resources, and significant bacterial diversity are characteristic of marine ecosystems.
- A table provides an overview of estimates of species for several ecologically significant groups.
Concepts (page 35)
- Biodiversity's hierarchical aspects are displayed: genetic, specific, and ecosystem diversity.
Concepts (page 36)
- Ecosystem biodiversity has three dimensions: composition (present organisms), structure (organization), and function (processes).
Concepts (page 37)
- Prokaryotes (bacteria) and Archaea are distinct forms of life.
- Eukaryotes encompass various organisms including protists, plants, fungi, and animals, each with distinct characteristics and evolutionary milestones.
- A time scale depicts major evolutionary events.
Concepts (page 38)
- Defining an ecosystem involves biotope (physical environment) and biocenosis (living component: micro- and macro-organisms).
Concepts (page 39)
- Organisms need energy and chemical elements (e.g., nutrients) for survival.
Concepts (page 40)
- Energy acquisition by microorganisms is through photosynthesis (using light) or chemosynthesis (using chemical substances or energetic substrates).
Concepts (page 41)
- Metabolism involves anabolism (building up larger molecules) and catabolism (breaking down larger molecules).
- ATP is crucial in energy transfer within these metabolic processes.
Concepts (page 42)
- Photosynthesis is a process where chlorophyll-containing photosynthetic cells synthesize organic molecules using light energy.
Concepts (page 43)
- The diagram illustrates the location of chlorophyll, pigments, and active sites in photosynthesis.
Concepts (page 44)
- Cellular respiration and fermentation are processes that differ based on the presence or absence of O2.
- These diagrams illustrate the overall pathways.
Concepts (page 45)
- Table outlines the different metabolic strategies of heterotrophic microorganisms based on their resource.
Concepts (page 46, 47)
- Respiration in bacteria is demonstrated via a diagram detailing aerobic respiration pathways.
Concepts (page 48)
- Anaerobic respiration employs alternative electron acceptors (e.g., nitrate, nitrite, sulfur) compared to the oxygen-based process of aerobic respiration.
- The role of methanogens and sulfate bacteria in anaerobic respiration is highlighted, along with their reliance on organic substrates or H+ as electron acceptors.
Concepts (page 49)
- Various organisms use different electron acceptors during anaerobic respiration.
- Processes like nitrate reduction and sulfate reduction are distinct examples of anaerobic respiration.
Concepts (page 50)
- Table comparing the three primary metabolic processes (aerobic respiration, anaerobic respiration, and fermentation).
- The use of organic or inorganic electron acceptors during respiration as a differentiating factor between these processes is highlighted, along with potential ATP production.
Concepts (page 51)
- Fermentation utilizes glycolysis but does not proceed through the TCA cycle or electron transport chain.
- Fermentation results in lower ATP production compared to respiration, with the energy remaining within the fermentation products.
Concepts (page 52)
- Diagram shows various fermentation end products from different organisms.
Concepts (page 53)
- Two major trophic categories of organisms are presented (autotrophs and heterotrophs).
- Autotrophs synthesize their organic substances from inorganic materials (e.g., minerals and CO2), while heterotrophs rely on organic matter.
Concepts (page 54)
- Heterotrophs are divided into consumers (herbivores, carnivores, parasites) and decomposers (bacteria, fungi).
- Their roles contribute to the recycling of organic waste returned to mineral state, which is reused by producers.
Concepts (page 55)
- Diagram illustrating the flow of energy through different trophic levels (producers, primary consumers, secondary consumers, tertiary consumers).
- Carbon flow, energy losses, and the roles of bacteria/fungi as decomposers are highlighted.
Concepts (page 56)
- Terminology related to the source of carbon, energy, and electron acceptors is discussed for microbial metabolism.
Concepts (page 57)
- Inorganic nutrients include elements/molecules without carbon and hydrogen (e.g., minerals, oxygen, and water).
- Organic nutrients include carbon and hydrogen-containing molecules produced by living organisms.
Concepts (page 58)
- Organisms can be chemoautotrophs (using inorganic chemicals as energy source and carbon source) or phototrophs (using light as energy with different carbon sources).
Concepts (page 59)
- Chemoorganotrophic metabolism is related to the consumption of organic compounds to obtain energy.
- ATP production and biosynthesis depend on the electron and proton-motive force for both aerobic and anaerobic respiration.
Concepts (page 60)
- Chemolithotrophic metabolism is related to the use of inorganic compounds as sources of energy and carbon.
- Electron and proton-motive force are critical in generating ATP for biosynthesis reactions.
Concepts (page 61)
- Photoheterotrophs utilize light as an energy source and organic materials as a carbon source.
- Photoautotrophs utilize light as an energy source and CO2 as a carbon source for biomass synthesis.
Concepts (page 62)
- Different metabolic strategies (chemoautotrophy, photoheterotrophy, photoautotrophy) for energy and carbon usage in different organisms are summarized.
- Key components of these strategies, including electron acceptors, are identified.
Concepts (page 63)
- Nitrogen, phosphorus, and sulfur are critical nutrients for microorganisms, needed for synthesizing essential molecules like amino acids, purines, pyrimidines, carbohydrates, lipids, and enzymatic cofactors.
- Microorganisms use diverse methods to acquire these elements from their environment.
Concepts (page 64)
- Phosphorus is a crucial element incorporated into essential cellular components such as nucleic acids, phospholipids, and nucleotides.
- Inorganic phosphate is typically used directly by bacteria.
- Uptake of inorganic phosphate can occur through specific porin channels in the outer bacterial membrane. Active transport processes exist if phosphate levels are low.
Concepts (page 65)
- A diagram depicts the low-affinity phosphate transporter in its natural context.
- The active transport and low-affinity phosphate transporters function on the bacterial membranes.
Concepts (page 66)
- Sulfur is a critical component in various organic molecules (amino acids, carbohydrates, vitamins) and microorganisms typically utilize sulfate as a sulfur source.
- Sulphate is reduced anabolically by microorganisms to acquire forms of sulfur integral to their survival and function.
Concepts (page 67)
- Microorganisms can associate with each other in different ways (ectosymbiosis, consortium, endosymbiosis, ecto/endosymbiosis) forming various biological relationships.
Concepts (page 68)
- This table defines different symbiotic interaction types: commensalism, ecto-epi/exosymbiosis, endosymbiosis, facultative symbiosis, mutualism, obligate mutualism, parasitism, pathogenesis, prokaryote, symbiosis, symbiont, and symbiomics.
Concepts (page 69)
- Symbiosis is the association between two or more different species, often resulting in a mutually beneficial relationship, illustrated in a diagram.
Concepts (page 70)
- A historical context of the term symbiosis is given, emphasizing its broad definition and including close biological relationships in its context.
- Lichen symbiosis is specifically analyzed; this highlighted the close relationships between fungi and photoautotrophs.
Concepts (page 71)
- Microbial associations can be permanent or intermittent, exhibiting different roles (positive: mutualism, protocooperation, commensalism and negative: predation, parasitism, competition, amensalism).
Concepts (page 72)
- Mutualism, involving a mutually beneficial relationship, occurs between separate species, and an illustration of this is displayed.
Concepts (page 73)
- Mutualism is a mandatory relationship where the mutualist and guest depend metabolically on each other, illustrated by the protozoa-termite relationship.
Concepts (page 74)
- Mutualism is demonstrated by the lichen association of photoautotrophic microorganisms (cyanobacteria or algae) and fungi; in lichens, the fungal partner is identified as mycobiotic and the algae/cyanobacterium as phycobiotic.
Concepts (page 75)
- Mutualism is discussed in the context of lichen symbiosis, where the fungus obtains organic carbon from the autotrophic organism (e.g., cyanobacteria) while the autotroph benefits from water and protection from the fungus.
- Lichens are sensitive to environmental pollutants including ozone and sulfur dioxide.
Concepts (page 76-82)
- Fungal-bacterial associations, specifically lichen symbiosis, are the focus of research in modern microbial ecology.
- Microbial communities in lichen holobiont are complex and diverse.
- Detailed examination of these microbial communities is now possible due to molecular techniques (FISH and microscopy).
Concepts (page 83)
- Protocooperation is a mutually beneficial relationship not mandatory like mutualism.
Concepts (page 84-87)
- Examples of protocooperation: the interaction between Desulfovibrio and Chromatium, nitrogen-fixing microorganisms and cellulolytic organisms, and the Pompei worm (Alvinella pompejana).
- Protocooperation allows several organisms to thrive in unique environments and interactions.
- The shrimp Rimicaris exoculata, devoid of eyes, thrives with chemolithotrophic bacteria in specific conditions, illustrating protocooperation.
Concepts (page 88)
- Example of protocooperation where nematodes in sulphide-rich marine sediments host sulphide-oxidizing bacteria.
Concepts (page 89)
- Protocooperation can also involve quorum sensing where microorganism populations control abundance via specific self-inducing compounds.
Concepts (page 90, 91)
- Commensalism is defined as a symbiotic association where one organism benefits while the other (host) is unaffected.
- This interaction is often unidirectional where the host and the commensal share resources from/by the host. The close proximity allows organisms to feed or reside on the host without affecting the host in either a positive or negative manner.
Concepts (page 92-95)
- Commensalism is defined as a non-harmful relationship where one organism benefits while the other is unaffected. Examples of commensal relationships include various species interactions where one benefits from the available resources of another.
- Commensalism is observed in microbial communities colonizing the human body or other animal/plant exterior.
Concepts (page 96, 97)
- Predation is a relationship where one organism (predator) captures and consumes another (prey).
- This leads to the prey's death, as demonstrated by predatory bacteria like Bdellovibrio, Vampirococcus, and Daptobacter.
Concepts (page 98-101)
- Predation in microbial systems involves the digestion of prey organisms by predators like flagella and cilia releasing and making nutrients available for other organisms in the microbial loop. The consumption of prey often increases the growth and renewal rate of the community.
- Parasitism is a symbiotic relationship where one organism (the parasite) gains benefit while the other (host) is harmed. Intracellular living of various bacteria within a specific host can provide protection.
Concepts (page 102, 103)
- Parasitism is a complex interaction where parasite benefits and the host is often negatively impacted. The relation varies among different species depending on the stability of the equilibrium between organisms. Pathogens are examples of parasites.
Concepts (page 104, 105)
- Parasitism (e.g., from virus to bacterial effects) and interactions can change in different environments from harsh conditions to optimal conditions.
- Amensalism is a nonbeneficial relationship where one organism negatively impacts another. This negative impact can stem from antibiotic production.
Concepts (page 106, 107)
- Amensalism involves one organism being negatively affected by the presence or activity of another; this includes producing compounds that harms the other organism in the presence of the harmful substance.
- Competition involves organisms from the same species or different species competing for the same available resource. This often leads to the exclusion of one species.
Concepts (page 108, 109)
- Microbial interactions aren't isolated events, but they influence each other impacting larger ecosystems.
- Illustrated by an interaction between microalgae and microbes in aquatic sediments showing various essential elements critical to the interaction.
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Description
Explore the intricate relationships and interactions within microbial communities through this quiz. It covers important concepts such as symbiosis, the differences between microbial ecology and environmental microbiology, and the impact of microbial interactions on health and the environment.