Microbial Diversity Overview

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Questions and Answers

What structure is always derived from cellular membranes when present in viruses?

  • Capsid
  • Envelope (correct)
  • Tail fibers
  • Base plate

Which type of virus is classified as infecting bacteria?

  • Enveloped viruses
  • Plant viruses
  • Fungal viruses
  • Bacteriophages (correct)

In the structure of a bacteriophage, which component is responsible for attaching to the bacterial surface?

  • Tail
  • Capsid
  • Head
  • Base Plate (correct)

What primarily determines the infective nature of a virus?

<p>The nucleic acid (D)</p> Signup and view all the answers

What is the primary function of tail fibers in a bacteriophage?

<p>Host recognition and attachment (D)</p> Signup and view all the answers

Which category do herpes and influenza viruses belong to?

<p>Animal viruses (C)</p> Signup and view all the answers

Which part of the bacteriophage contains the viral nucleic acid?

<p>Head (B)</p> Signup and view all the answers

What type of viruses primarily infect plants and algae?

<p>Plant viruses (B)</p> Signup and view all the answers

What is the primary route of transmission for poliovirus?

<p>Fecal-oral route (C)</p> Signup and view all the answers

Which type of genome does HIV possess?

<p>Single-stranded RNA (C)</p> Signup and view all the answers

What is the shape of the poliovirus capsid?

<p>Icosahedral symmetry (B)</p> Signup and view all the answers

Which protein is essential for HIV's ability to bind to host cells?

<p>gp120 (A)</p> Signup and view all the answers

What is the consequence of HIV targeting white blood cells?

<p>It compromises the immune system (B)</p> Signup and view all the answers

What type of genome does the ADIS virus possess?

<p>Single-stranded RNA (A)</p> Signup and view all the answers

Which of the following viruses is classified as a retrovirus?

<p>ADIS Virus (B)</p> Signup and view all the answers

What structural feature is unique to the ADIS virus?

<p>Reverse transcriptase enzyme (C)</p> Signup and view all the answers

Which of the following diseases is associated with the ADIS virus?

<p>Acquired immunodeficiency syndrome (AIDS) (C)</p> Signup and view all the answers

What is the envelope status of the Poliovirus?

<p>Non-enveloped (B)</p> Signup and view all the answers

What role do microbes play in environmental monitoring?

<p>They provide early warnings of environmental disturbances. (D)</p> Signup and view all the answers

What significant contribution did Carl Woese make to the classification of organisms?

<p>He identified a new group of organisms called Archaea. (B)</p> Signup and view all the answers

How are prokaryotes classified in the modern classification system?

<p>They comprise Archaea and Bacteria. (B)</p> Signup and view all the answers

What aspect of microbial life informs the search for extraterrestrial life?

<p>The evolutionary adaptations of microbes on Earth. (D)</p> Signup and view all the answers

What distinguishes Archaea from Bacteria at a molecular level?

<p>Differences in nucleotide base sequences in 16S rRNA. (B)</p> Signup and view all the answers

Why are viruses considered a separate entity in biological classification?

<p>They display both living and non-living characteristics. (C)</p> Signup and view all the answers

What was primarily used for classifying organisms until the 1970s?

<p>Physiological differences. (C)</p> Signup and view all the answers

What are the domains classified under the three-domain system?

<p>Archaea, Bacteria, and Eukarya. (A)</p> Signup and view all the answers

Which family of bacteriophages has a contractile tail?

<p>Myoviridae (B)</p> Signup and view all the answers

What type of nucleic acid do bacteriophages from the Caudovirales order typically utilize?

<p>Linear ds DNA (C)</p> Signup and view all the answers

Which of the following bacteriophage families is characterized by having a short tail?

<p>Podoviridae (B)</p> Signup and view all the answers

Which bacteriophage employs tail fibers for binding to bacterial surface receptors?

<p>Lambda Phage (D)</p> Signup and view all the answers

What is the general structure of T4 Bacteriophage?

<p>Nonenveloped with a complex structure (B)</p> Signup and view all the answers

Which statement about Caudovirales is incorrect?

<p>Myoviridae has a non-contractile tail. (D)</p> Signup and view all the answers

What distinguishes the viruses in the Caudovirales order?

<p>Presence of tail structures (C)</p> Signup and view all the answers

Which family of bacteriophages can be classified as temperate phages?

<p>Siphoviridae (C)</p> Signup and view all the answers

What type of genetic material does the Tobacco Mosaic Virus (TMV) possess?

<p>Single-stranded positive-sense RNA (D)</p> Signup and view all the answers

Which structural feature is NOT characteristic of poxviruses?

<p>Icosahedral symmetry (A)</p> Signup and view all the answers

Which type of virus is M13 phage classified as?

<p>Filamentous with single-stranded DNA (D)</p> Signup and view all the answers

What is the primary function of the coat proteins in Tobacco Mosaic Virus?

<p>To form the helical core around the RNA (C)</p> Signup and view all the answers

What infection characteristic is associated with poxviruses?

<p>Development of vesicular skin lesions (D)</p> Signup and view all the answers

How does M13 phage bind to bacterial cells?

<p>By binding to surface pili (A)</p> Signup and view all the answers

What is the approximate size of Tobacco Mosaic Virus?

<p>300 nm in length and 18 nm in diameter (C)</p> Signup and view all the answers

Which of the following is true regarding the structural symmetry of poxvirus capsids?

<p>Poxviruses show a lack of typical capsid symmetry (D)</p> Signup and view all the answers

Flashcards

Microbial Communities

Populations of microbes (bacteria, archaea, and other microorganisms) interacting in an environment.

Environmental Monitoring

Observing changes in the environment, often using microbes as indicators.

Bioremediation

Using microbes to clean up pollution or contamination.

Evolutionary Insights

Using microbes to understand the history of life on Earth.

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Three-Domain System

Modern classification of life into Bacteria, Archaea, and Eukarya.

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Prokaryotes

Organisms (Bacteria and Archaea) lacking a nucleus and other membrane-bound organelles.

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Eukaryotes

Organisms (e.g., plants, animals) with a nucleus and other membrane-bound organelles.

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16S rRNA

A type of ribosomal RNA used to classify organisms based on their genetic similarity.

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Viruses

Biological entities showing both living and non-living characteristics

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Nucleocapsid

Structure formed by the capsid in close contact with the nucleic acid of a virus.

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Enveloped Virus

A virus with an outer layer (envelope) derived from the host cell's membrane.

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Naked Virus

A virus without an outer envelope.

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Viral Infectivity

The ability of a virus to cause infection, determined by the nucleic acid.

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Viral Host Specificity

The ability of a virus to target specific types of hosts, determined by the protein coat.

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Bacteriophage

A virus that infects bacteria.

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Bacteriophage Head

Part of a bacteriophage containing the viral DNA/RNA and enclosed protein shell.

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Bacteriophage Tail

The structure that helps a bacteriophage inject its genetic material into the bacterial cell.

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Bacteriophage Base Plate

Structure at the end of the tail that attaches to the bacterial surface.

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Bacteriophage Tail Fibers

Structures that help a bacteriophage recognize and attach to its host bacterial cell.

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Viral Classification

System for organizing and identifying viruses based on their characteristics (type of host).

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Poxvirus Core

The dumbbell-shaped structure within a poxvirus that contains the viral genome and proteins for replication/transcription.

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Poliovirus Genome

Single-stranded positive-sense RNA that directs poliovirus replication.

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Poliovirus Capsid

Outer protein shell with icosahedral symmetry that protects the viral RNA.

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HIV Genome

Two identical strands of positive-sense RNA that make up the HIV virus's genetic material.

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HIV Capsid (Structure)

Conical/bullet shaped structure enclosing the HIV RNA and replication enzymes. Includes p17 and p24 protein layers.

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HIV Envelope

Lipid layer from host cells with glycoproteins (gp120 & gp41) essential for HIV entry.

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HIV Enzymes

Reverse transcriptase, integrase, and protease for HIV replication and integration into host cells.

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M13 Phage

A filamentous bacteriophage, nonenveloped, with a single-stranded DNA genome (ssDNA).

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Bacteriophage

A virus that infects bacteria.

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Tobacco Mosaic Virus (TMV)

A rod-shaped plant virus that infects tobacco plants, causing mosaic patterns on leaves. Its genetic material is single-stranded positive-sense RNA (+ssRNA).

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Viral Capsid

Protein coat of a virus, surrounding the genetic material (DNA or RNA).

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Poxvirus

A group of large, brick-shaped animal viruses with a complex internal structure, double-stranded DNA genome (dsDNA), and an envelope.

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Single-stranded DNA (ssDNA)

A type of nucleic acid where the DNA molecule exists as one strand.

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Double-stranded DNA (dsDNA)

A type of nucleic acid where the DNA molecule consists of two complementary strands.

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Viral Envelope

A lipid membrane that surrounds some viruses and is derived from the host cell.

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Bacteriophage structure

Bacteriophages have a head containing nucleic acid (DNA or RNA) and a tail with tail fibers for attachment to bacterial cells.

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Caudovirales order

A large order of bacteriophages, containing 96% of all known bacteriophages; includes Myoviridae, Podoviridae and Siphoviridae.

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Myoviridae family

Bacteriophages in this family have contractile tails.

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Podoviridae family

Bacteriophages in this family have short, non-contractile tails.

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Siphoviridae family

Bacteriophages in this family have long, non-contractile tails.

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Tail Fibers

Structures on the tail of bacteriophages used for attachment to bacterial cells.

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Baseplate

Part of the tail involved in attachment to bacterial cell wall.

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Linear ds DNA

Double-stranded, linear DNA, found as the genetic material in some bacteriophages.

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T4 Phage

An example of a bacteriophage in the Myoviridae family, possessing a complex structure and contractile tail.

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T7, T3 Phage

Bacteriophages belonging to Myoviridae family with complex structures and contractile tails.

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Lambda Phage

A temperate bacteriophage in the Siphoviridae family that utilizes tail fibers to bind to bacterial surface receptors.

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ADIS Virus Family

Retroviridae, a family of viruses with a single-stranded RNA genome.

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ADIS Virus Envelope

Yes, with lipid bilayer and glycoproteins (gp120, gp41).

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ADIS Virus Shape

Spherical

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ADIS Virus Unique Feature

Reverse transcriptase enzyme.

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ADIS Associated Disease

Acquired immunodeficiency syndrome (AIDS).

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Poxvirus Genome

Double-stranded DNA (dsDNA).

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Poxvirus Envelope

Yes, with lipid bilayer and surface proteins.

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Poxvirus Shape

Brick-shaped or ovoid.

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Poxvirus Unique Features

Complex with outer envelope, surface tubules, and lateral bodies.

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Poliovirus Genome

Single-stranded positive-sense RNA (SSRNA).

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Poliovirus Envelope

No, non-enveloped.

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Poliovirus Shape

Spherical or icosahedral.

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Poliovirus Unique Features

Non-enveloped, icosahedral capsid; no reverse transcriptase.

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Study Notes

Microbial Diversity

  • Microorganisms existed before macroorganisms, more than 3 billion years ago.
  • Antonie van Leeuwenhoek discovered microorganisms in the 17th century.
  • Microbial diversity is complex and includes variety of species, metabolic and ecological roles.
  • Microorganisms consist of Bacteria, Archaea, Fungi, Protozoa, Algae, and Viruses.
  • Microbial diversity is critical for maintaining ecosystem health, nutrient cycling, and life on Earth.

Importance of Microbial Diversity

  • Ecological Stability: Microbes are crucial in biogeochemical cycles (carbon, nitrogen, sulfur).
  • Human Health: Human microbiome is essential for health (digestion, vitamins, immune system). Loss of diversity can lead to diseases (obesity, allergies).
  • Biotechnology and Industry: Microbes are used in biotechnology for antibiotics, enzymes, biofuels, and fermented foods. They also play a role in environmental monitoring and bioremediation.
  • Evolutionary insights: Understanding microbial evolution helps understand the history and future of life on Earth.

Classification of Organisms

  • Until the 1970s, classification was based on physiological differences.
  • 1970s: Techniques became available to analyze nucleic acids, especially ribosomal RNA (rRNA).
  • 1980s: Woese used rRNA comparisons to propose three domains: Archaea, Eukarya, Bacteria.
  • These domains represent prokaryotes (Archaea and Bacteria) and eukaryotes (Eukarya).

Viruses

  • Viruses are considered a separate group due to living and non-living characteristics.

  • Viruses are ultramicroscopic, obligate intracellular parasites of bacteria, plants, and animals.

  • Viruses lack cellular structure, relying on host cells for reproduction.

  • Viruses can have either RNA or DNA as their genome.

  • Viruses are categorized by shape (polyhedral, helical, or complex).

  • Viruses need a host cell to survive; they don't reproduce independently.

  • Viral structure: capsid (protein coat), genome (DNA or RNA), and sometimes an envelope (derived from host cells).

  • Viral classification: Plant viruses, Animal viruses, Fungal viruses, Bacterial viruses.

  • Bacteriophages (phages) are viruses that infect bacteria.

  • Bacteriophages are diverse and ubiquitous, affecting host bacteria in many ways.

  • Diversity is based on genome structure, tail structures, and modes of host infection.

  • Tobacco Mosaic Virus (TMV): rod-shaped, infects tobacco and similar plants, single-stranded RNA genome.

  • Poxviruses: large, complex, double-stranded DNA genome, brick-shaped, infect humans and animals, Examples: smallpox.

  • Poliovirus: Icosahedral shape infecting humans, single-stranded RNA genome, causes poliomyelitis.

  • AIDS Virus: has a positive-sense single-stranded RNA genome, surrounded by a lipid envelope, affects humans.

Table of Differences

  • A table differentiates animal viruses (Poxviruses, Poliomyelitis viruses, and AIDS viruses) based on genome type, envelope, shape, and associated diseases.

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