18 Questions
What role does the cellular location of peptidoglycan synthesis play in determining cell shape?
Peptidoglycan forms only at the central septum
What is the role of FtsZ localization placement in cell shape determination?
It plays a role in guiding cell division at the central septum
In a microbial growth curve, what phase follows the lag phase?
Exponential (log) phase
What defines population growth in microbial cultures?
Increase in cell size and number simultaneously
What type of culture involves incubation in a closed vessel with a single batch of medium?
Batch culture
How is the microbial growth curve typically plotted for microorganisms cultivated in liquid culture?
Logarithm of cell number versus time
Which method involves spreading a diluted sample of bacteria over a solid agar surface or pouring it into a Petri plate?
Standard Plate Counts
What is the primary purpose of the membrane filter method in microbiology?
Trapping bacteria from aquatic samples
Which technique is most suitable for determining the number of bacteria in a sample based on colonies grown on a membrane?
Membrane Filter Method
What is one key parameter to consider when aiming to control or encourage microbial growth?
pH level
In microbiology, what does CFU stand for?
Colony Forming Units
What is the main advantage of the membrane filter method over the standard plate counts technique?
Higher sensitivity
What is the purpose of spread plate method in microbiology?
To evenly spread a small volume of diluted mixture over an agar plate
Why does the pour plate method use liquid agar?
To facilitate mixing of the sample with agar before pouring into dishes
In microbial population size measurement, why are plate count results expressed in terms of colony forming units (CFUs)?
To avoid direct counting of individual microbes
What is the purpose of direct counts and membrane filter technique in measuring microbial growth?
To estimate total microbial population size directly
Which technique is most suitable for measuring changes in cell mass during microbial growth?
Cell mass measurements
What is the main advantage of using viable counting methods for measuring microbial growth?
Focus on counting viable and culturable cells
Test your knowledge on how microbes change shape under certain circumstances and determine cell shape based on their cellular location of peptidoglycan synthesis. Explore concepts such as Coccus and Rod cell shapes, as well as the role of FtsZ and MreB proteins in shaping microbial cells.
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