Microbial Activity in Food Production
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Questions and Answers

What is a primary benefit of reducing pH in food preservation?

  • Enhances food color
  • Improves food texture
  • Inhibits microbial growth (correct)
  • Increases nutrient content

What is the main purpose of fermentation in food processing?

  • To convert sugars into organic acids (correct)
  • To freeze food for preservation
  • To enhance water activity
  • To increase the pH of the food

Which lactic acid bacteria is noted for its ability to grow at low pH levels?

  • Bacillus subtilis
  • Lactobacillus (correct)
  • Listeria monocytogenes
  • Escherichia coli

What method involves chopping food into small pieces to ensure acid penetration?

<p>Pickling (C)</p> Signup and view all the answers

In the fermentation process, what happens to the sugar concentration?

<p>It decreases to low levels (D)</p> Signup and view all the answers

Which food is commonly fermented using lactic acid bacteria?

<p>Cabbage (B)</p> Signup and view all the answers

What is the typical pH achieved during successful fermentation of food?

<p>Around 3.4 (B)</p> Signup and view all the answers

Which of the following describes a limitation of acid diffusion in oily foods?

<p>Acids cannot penetrate deeply (B)</p> Signup and view all the answers

What is the importance of shredding cabbage into small pieces during sauerkraut production?

<p>It increases the surface area for better diffusion. (A)</p> Signup and view all the answers

What percentage of salt is generally used in sauerkraut production?

<p>2-3% w/w (A)</p> Signup and view all the answers

In the vinegar fermentation process, what does the Acetobacter bacteria require for its function?

<p>Oxygen (A)</p> Signup and view all the answers

Which method describes the semi-continuous fermentation process used in traditional French vinegar-making?

<p>Orleans process (C)</p> Signup and view all the answers

What is the primary reason for draining off most of the vinegar during the Orleans process?

<p>To allow Acetobacter to have sufficient oxygen. (B)</p> Signup and view all the answers

In a trickle fermenter, what role do the wood shavings or substrate play?

<p>They provide a surface for Acetobacter growth. (D)</p> Signup and view all the answers

What toxic compound is generated during sauerkraut fermentation that must be managed?

<p>Acetic acid (A)</p> Signup and view all the answers

How does the proton gradient benefit the cell during sauerkraut fermentation?

<p>By storing energy for later use. (D)</p> Signup and view all the answers

Flashcards

Fermentation

Microorganisms like bacteria and yeasts can transform food through controlled fermentation, leading to changes in flavor, texture and preservation.

Lactic Acid Fermentation

A process where microorganisms convert sugars or other carbohydrates into acids, like lactic acid, which lowers the pH of the food, making it less hospitable to harmful bacteria and extending its shelf life.

Water Activity (Aw)

A measure of the amount of free water available in food. It is lower in fermented foods due to the reduction in water activity by fermentation.

Organoleptic Changes

The changes in the appearance, taste, smell, and texture of food due to fermentation.

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Acidification

A chemical process where organic acids are produced by microorganisms, leading to a decrease in the pH of the food.

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Pickling

A method of food preservation that relies on diffusing acids into food from the outside. This works best for chopped foods with small pieces to allow for the penetration of acids.

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Fermentation (Internal)

A process involving microorganisms that act directly inside the food, breaking down sugars into acids, without relying on diffusion from the outside.

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Lactobacillus

A type of bacteria that thrives in low pH environments, often used for fermentation. It produces lactic acid, contributing to the distinct sour taste in fermented foods.

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Vinegar fermentation

A type of fermentation where acetic acid bacteria convert ethanol (alcohol) into acetic acid, the main component of vinegar.

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Orleans process

A traditional French method of vinegar production that involves a semi-continuous process using wooden barrels with a viewing port. Wine is added, acetobacter bacteria are introduced, and the vinegar is drained off when sufficiently acidic.

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Acetobacter bacteria

Acetic acid bacteria found in the Orleans process, responsible for converting ethanol to acetic acid, are introduced either from a starter culture or left over from previous fermentation.

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Trickle fermenter

A modern, continuous method for vinegar production. Alcohol is fed into a fermenter filled with wood shavings or other substrate supporting acetobacter bacteria. Vinegar is continuously collected from the bottom.

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Solid state fermentation

A type of fermentation process where microorganisms grow on solid material, like the wood shavings in a trickle fermenter.

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Salting cabbage

The process of adding salt to fresh cabbage in sauerkraut production. This breaks down the cells and creates a favorable environment for fermentation.

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Ethanal

A chemical compound produced during sauerkraut fermentation that can lead to softening of the product. The chemical reaction involves the conversion of ethanal by aldehyde dehydrogenase into acetic acid.

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Study Notes

Beneficial Microbial Activity in Food Production

  • Microbial activity isn't always detrimental; it plays a crucial role in food production.
  • Microorganisms are used in fermenting various foods like meat, vegetables, and dairy products.
  • Single-cell protein (SCP) and vinegar production also involve microbial processes.

Preservative and Flavor Effects

  • Microorganisms create foods through preserving effects and flavor changes.
  • Preservative effects include lower pH and water activity (Aw).
  • Flavor effects involve organoleptic changes, such as alterations in texture.

Lowering pH for Microbial Control

  • Most microbes stop growing below pH 4.
  • Lactobacillus bacteria can thrive at lower pH levels and are utilized in fermentation.

Methods of Acidification

  • Pickling: Adding acid (acetic or lactic) to food. Requires chopping food into small pieces to promote acid diffusion from outside in.
  • Fermentation: Microbes work inside food, like vegetables or meat, to lower the pH. Does not rely on outward diffusion.

Fermented Vegetable Production

  • Sauerkraut:
    • Fresh cabbage is shredded thinly and mixed with salt.
    • Salt creates an environment for lactic acid bacteria, changing flavor, and lowering pH.
    • Salt concentration is crucial, with > 2% potentially softening the product.

Vinegar Fermentation Methods

  • Orleans Process (Traditional):

    • Semi-continuous, where wine is added, and vinegar is drained, utilizing leftover bacteria from the previous batch as starter culture.
    • Acetobacter is housed at the top due to oxygen requirement.
  • Trickle Fermenter (Modern):

    • Continuous fermentation system.
    • Alcohol is added continuously, and vinegar is collected continuously.
    • Solid substrate, like wood shavings, supports the growth of Acetobacter.

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Description

Explore the essential role of beneficial microbial activity in food production techniques, including fermentation and preservation. Learn how microorganisms contribute to flavors and textures while effectively controlling microbial growth through acidification methods. This quiz covers various applications from pickling to single-cell protein production.

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