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Questions and Answers
What is the cell size of yeast?
What is the cell size of yeast?
What temperature range do most yeasts grow best at?
What temperature range do most yeasts grow best at?
What is the main method of reproduction for molds?
What is the main method of reproduction for molds?
Which type of microbes can grow in both aerobic and anaerobic environments?
Which type of microbes can grow in both aerobic and anaerobic environments?
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What is destroyed by heat but resistant to dry conditions?
What is destroyed by heat but resistant to dry conditions?
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Which type of microorganism is classified as prokaryotic?
Which type of microorganism is classified as prokaryotic?
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What is the biology of small living organisms (microorganisms) relating to foods known as?
What is the biology of small living organisms (microorganisms) relating to foods known as?
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Which microorganism is responsible for producing phycocyanin, a type of antioxidant?
Which microorganism is responsible for producing phycocyanin, a type of antioxidant?
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Which microorganism is known for producing omega-6 & omega-3 polyunsaturated fatty acids (PUFAs)?
Which microorganism is known for producing omega-6 & omega-3 polyunsaturated fatty acids (PUFAs)?
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Which factor does not affect microbial growth?
Which factor does not affect microbial growth?
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What is the mechanism of bacteria gram staining?
What is the mechanism of bacteria gram staining?
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What is the size comparison between viruses and bacteria?
What is the size comparison between viruses and bacteria?
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How do some viruses replicate?
How do some viruses replicate?
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Which microalgae is known for producing Astaxanthin?
Which microalgae is known for producing Astaxanthin?
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What type of organisms are Protozoa?
What type of organisms are Protozoa?
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Which kingdom includes single-celled organisms living in extreme conditions, such as thermophiles?
Which kingdom includes single-celled organisms living in extreme conditions, such as thermophiles?
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What characteristic is used to classify microorganisms?
What characteristic is used to classify microorganisms?
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Which type of microorganism can be both single-celled or multi-celled and exhibit different modes of growth like photoautotrophic, heterotrophic, and mixotrophic?
Which type of microorganism can be both single-celled or multi-celled and exhibit different modes of growth like photoautotrophic, heterotrophic, and mixotrophic?
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What type of microorganisms can cause foodborne illnesses and food spoilage?
What type of microorganisms can cause foodborne illnesses and food spoilage?
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How are microorganisms classified into kingdoms?
How are microorganisms classified into kingdoms?
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Which kingdom includes single-celled organisms that often live in extreme conditions, such as thermophiles?
Which kingdom includes single-celled organisms that often live in extreme conditions, such as thermophiles?
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What are examples of foodborne diseases caused by microorganisms?
What are examples of foodborne diseases caused by microorganisms?
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How are microorganisms classified based on characteristics?
How are microorganisms classified based on characteristics?
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What do fungi include?
What do fungi include?
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How do bacteria impact daily life?
How do bacteria impact daily life?
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What are the six kingdoms into which microorganisms are classified?
What are the six kingdoms into which microorganisms are classified?
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What are the characteristics used to classify microorganisms?
What are the characteristics used to classify microorganisms?
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How are microorganisms detected in food?
How are microorganisms detected in food?
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What are the examples of foodborne diseases caused by microorganisms?
What are the examples of foodborne diseases caused by microorganisms?
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What are the different types of growth exhibited by Protista?
What are the different types of growth exhibited by Protista?
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Explain the classification of microorganisms mentioned in the text.
Explain the classification of microorganisms mentioned in the text.
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What are the factors that affect microbial growth according to the text?
What are the factors that affect microbial growth according to the text?
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Provide examples of beneficial applications of microorganisms in daily life.
Provide examples of beneficial applications of microorganisms in daily life.
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What are the nutritional supplements and antioxidants mentioned to be produced by microorganisms?
What are the nutritional supplements and antioxidants mentioned to be produced by microorganisms?
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Explain the production of fermented food by microorganisms as discussed in the text.
Explain the production of fermented food by microorganisms as discussed in the text.
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Explain the characteristics of molds and their method of reproduction.
Explain the characteristics of molds and their method of reproduction.
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Describe the growth requirements of yeasts and their importance in the food industry.
Describe the growth requirements of yeasts and their importance in the food industry.
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Explain the oxygen requirements of microbes and provide examples of aerobic and anaerobic growth.
Explain the oxygen requirements of microbes and provide examples of aerobic and anaerobic growth.
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Discuss the role of agar plates/broth in microbial maintenance.
Discuss the role of agar plates/broth in microbial maintenance.
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Explain the importance of yeasts in the food industry, with a specific example.
Explain the importance of yeasts in the food industry, with a specific example.
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- What is the mechanism of bacteria gram staining?
- What is the mechanism of bacteria gram staining?
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- What is the size of viruses compared to bacteria cells?
- What is the size of viruses compared to bacteria cells?
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- What is the main method of reproduction for green microalgae like Chlamydomonas and Chlorella?
- What is the main method of reproduction for green microalgae like Chlamydomonas and Chlorella?
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- What are some products known to be produced by specific microalgae?
- What are some products known to be produced by specific microalgae?
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- What is an example of food poisoning caused by Protozoa?
- What is an example of food poisoning caused by Protozoa?
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- What are the characteristics of viruses compared to bacteria cells?
- What are the characteristics of viruses compared to bacteria cells?
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- How do humans usually get infected with viruses that cause foodborne illnesses?
- How do humans usually get infected with viruses that cause foodborne illnesses?
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- What is primary contamination of food and give an example?
- What is primary contamination of food and give an example?
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- What is secondary contamination of food and who or what causes it?
- What is secondary contamination of food and who or what causes it?
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- Name three common viruses causing foodborne illness.
- Name three common viruses causing foodborne illness.
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What is the purpose of the Thermal Death Time (TDT) curve?
What is the purpose of the Thermal Death Time (TDT) curve?
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What does the D-value represent in food preservation?
What does the D-value represent in food preservation?
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How does refrigeration affect microbial growth?
How does refrigeration affect microbial growth?
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What is the purpose of Modified Atmosphere Packaging (MAP) in food preservation?
What is the purpose of Modified Atmosphere Packaging (MAP) in food preservation?
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How does dehydration inhibit microbial growth?
How does dehydration inhibit microbial growth?
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In food preservation, what are the aims and principles of food preservation?
In food preservation, what are the aims and principles of food preservation?
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What are the symptoms and transmission methods of Norovirus?
What are the symptoms and transmission methods of Norovirus?
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What are the symptoms and transmission methods of Rotavirus?
What are the symptoms and transmission methods of Rotavirus?
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What are the symptoms and onset time of Hepatitis A virus infection?
What are the symptoms and onset time of Hepatitis A virus infection?
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What are the symptoms and causes of foodborne illnesses related to molds?
What are the symptoms and causes of foodborne illnesses related to molds?
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- What is pasteurization and how does it work in food processing?
- What is pasteurization and how does it work in food processing?
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- What are the two main methods of pasteurization and their respective temperature and duration?
- What are the two main methods of pasteurization and their respective temperature and duration?
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- What is ultra-high temperature (UHT) pasteurization and how does it differ from regular pasteurization?
- What is ultra-high temperature (UHT) pasteurization and how does it differ from regular pasteurization?
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- What is sterilization in food processing and how is it achieved?
- What is sterilization in food processing and how is it achieved?
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- What is the significance of D value in measuring thermal-killing efficiency?
- What is the significance of D value in measuring thermal-killing efficiency?
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Study Notes
Microorganisms and Their Impact on Daily Life
- Microorganisms like bacteria, viruses, fungi, and protozoa can cause foodborne illnesses and food spoilage.
- Examples of foodborne diseases caused by microorganisms include cholera, avian influenza, and ciguatera food poisoning.
- Microorganisms can be detected in food using specific methods.
- Microorganisms are classified based on characteristics such as cell shape, size, and structure arrangement.
- The classification of microorganisms involves scientific and common names.
- The scientific name follows specific rules, such as italicizing or underlining the genus and species.
- Microorganisms are classified into six kingdoms, including Protista, Fungi, Archaebacteria, and Eubacteria.
- Protista can be single-celled or multi-celled and exhibit different modes of growth like photoautotrophic, heterotrophic, and mixotrophic.
- Fungi include both single-celled (yeasts) and multi-celled (molds) organisms that grow without light.
- Archaebacteria are single-celled organisms that often live in extreme conditions, such as thermophiles.
- Eubacteria, also single-celled, can live in various environments and can exhibit photoautotrophic, heterotrophic, or mixotrophic growth.
- Bacteria, which are prokaryotes and lack a membrane-bound nucleus, can impact daily life by causing food spoilage and diseases, serving as a source of drugs, and being a source of vitamins and nutrients.
Microbes in Biology
- Bacteria are identified based on cell structure and can be Gram positive (blue/purple) or Gram negative (red).
- Mechanism of bacteria gram staining involves fixed cells on a slide and staining with crystal violet-iodine complex.
- Viruses are obligate intracellular parasites and can be host-specific, with a size of 10nm compared to bacteria cell size of 1µm.
- Viruses replicate by inserting genetic materials into host cells, leading to lysis of the host cell wall.
- Some viruses, like Avian Influenza (Bird Flu) caused by H5N1 virus, can cause food poisoning.
- Microalgae are a large group of microbes found in various environments, including soil, fresh water, and marine environments.
- Microalgal cell structure includes a cell wall, chloroplast for photosynthesis, and mitochondrion for respiration.
- Green microalgae, such as Chlamydomonas and Chlorella, can be unicellular, motile or non-motile, and reproduce asexually or sexually.
- Chlorella zofingiensis is known for producing Astaxanthin, a red carotenoid used as a natural food colorant.
- Chlorella protothecoides produces Lutein, a yellow carotenoid used as a food colorant in egg yolk.
- Protozoa are single-celled, eukaryotic organisms that can be photoautotrophic, heterotrophic, or mixotrophic.
- Food poisoning caused by Protozoa includes Ciguatera, caused by Gambierdiscus toxicus.
Pasteurization and Sterilization in Food Processing
- Pasteurization is a thermal process used to inactivate enzymes and eliminate viable pathogenic microorganisms from heat-sensitive foods such as milk, fruit juice, wine, and egg.
- It involves heating the food to below 100˚C for a specific duration and then rapidly cooling it to slow down microbial growth, requiring refrigeration storage and providing a shelf life of 2-3 weeks.
- Pasteurization involves two main methods: low-temperature pasteurization at 63˚C for 30 minutes and high-temperature short time (HTST) pasteurization at 72˚C for 15 seconds, with the latter being more effective in retaining nutritional and sensory quality.
- HTST pasteurization of raw milk involves heating the milk briefly to 72 °C for about 15 seconds to kill disease-causing microbes, followed by rapid cooling and refrigeration storage.
- Ultra-high temperature (UHT) pasteurization of raw milk involves heating the milk to around 138 °C for 2-3 seconds, then rapidly cooling and packaging it in sterile, hermetically-sealed containers, providing a shelf-stable product for 6 months without requiring refrigeration until opened.
- Sterilization is a process that completely kills or removes all life forms, including bacteria and other organisms, and is commonly achieved through heating methods such as wet heating at 121°C for 15-20 minutes.
- Commercial sterilization combines heat and vacuum seal to destroy all spoilage and pathogenic microorganisms, including endospores of Clostridium botulinum, used in producing canned food through retort processing.
- Canning involves commercial sterilization of low acid foods at 121°C for a duration sufficient to inactivate Clostridium botulinum spores by 12 log and high acid foods at around 100°C to destroy both vegetative cells and spores of spoilage microorganisms.
- One log reduction indicates a 90% reduction in the microbial population, and 12 log reduction signifies a complete eradication of the microorganisms.
- Bacterial populations killed by heat or chemicals tend to die at constant rates, with the microbial death rate curve showing this constant death as a straight line.
- Three important values used to measure thermal-killing efficiency are D value (decimal reduction time), Z value (increase in temperature required to reduce D value by ten-fold), and F value.
- D value represents the time needed to reduce the number of a given microorganism by 90% at a specific temperature, and it decreases as the temperature increases, differing for different microbial species.
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Description
Test your knowledge of microbes in biology with this quiz. Explore topics such as bacteria cell structure, gram staining, virus replication, microalgae characteristics, and the classification of microorganisms. Learn about the impact of microorganisms on daily life, including foodborne illnesses and food spoilage.