Microarrays and Their Applications
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Questions and Answers

What are microRNAs (miRNAs)?

  • Transcripts that are unknown to scientists
  • Noise in high-density microarrays
  • Protein-coding genes in the genome
  • Small noncoding RNAs that regulate gene expression (correct)

How can microarrays be used to measure the expression of microRNAs?

  • By using probes specific to microRNAs (correct)
  • By studying protein-coding genes only
  • By increasing the density of microarray chips
  • By focusing on unknown transcripts

What are tiling arrays designed for?

  • Identifying unknown miRNAs
  • Measuring the abundance of all transcribed parts of the genome (correct)
  • Detecting noise in high-density microarrays
  • Studying protein-coding genes

What have scientists found using tiling arrays?

<p>Most parts of the human genome are transcribed (B)</p> Signup and view all the answers

What is proteomics the study of?

<p>All or many proteins (B)</p> Signup and view all the answers

What is the major difference between cDNA microarrays and oligonucleotide microarrays?

<p>The way the probes are prepared (D)</p> Signup and view all the answers

How has the term 'transcriptome' changed over time?

<p>From focusing on mRNA expression to studying all or many transcripts (C)</p> Signup and view all the answers

Which type of microarray is also known as cDNA microarray?

<p>cDNA microarray (B)</p> Signup and view all the answers

What is the main function of DNA microarrays in the field of functional genomics?

<p>Study gene expression patterns (D)</p> Signup and view all the answers

In what type of cell can DNA microarrays be used to identify altered gene expression, such as in cancer cells?

<p>Both prokaryotic and eukaryotic cells (A)</p> Signup and view all the answers

What aspect of gene expression can DNA microarrays help researchers analyze?

<p>Transcriptional regulation (B)</p> Signup and view all the answers

Which technique can DNA microarrays complement in the study of functional genomics?

<p>Mass Spectrometry (B)</p> Signup and view all the answers

What is the typical application of mass spectrometry mentioned in the text?

<p>Measuring mRNA abundances (B)</p> Signup and view all the answers

In mass spectrometry, what does aligning the peaks between different samples allow researchers to do?

<p>Detect differentially expressed proteins (C)</p> Signup and view all the answers

What does ChIP-Chip technique combine ChIP with, according to the text?

<p>DNA microarrays (A)</p> Signup and view all the answers

What is a primary focus of ChIP-Chip technique?

<p>Detecting transcription factor binding sites (A)</p> Signup and view all the answers

How does ChIP-Sequencing help in studying DNA-protein interactions?

<p>By sequencing DNAs bound by specific proteins (C)</p> Signup and view all the answers

What does ChIP-Chip technique help in studying according to the text?

<p>Transcription factor binding sites (A)</p> Signup and view all the answers

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