30 Questions
How are F plasmids involved in bacterial conjugation?
They encode gene products that play a role in transferring genetic material from one bacterium to another
What is the significance of Hfr bacterial cells in chromosomal mapping?
They allow the analysis of gene transfer direction and frequency
How are F primes different from F plasmids?
F primes can complement chromosomal mutations and aid in gene transfer between bacteria
What is the primary function of the gene products encoded by the F plasmid in bacterial conjugation?
Facilitating the transfer of genetic material between bacteria
How do bottlenecks impact genetic diversity in bacterial populations?
Limit genetic diversity by reducing the number of genetic variants in a population
Why is chromosomal mapping important in understanding bacterial genetics?
To establish the order and distance between genes on a chromosome
Which bacteria requires ComQ proteins for DNA binding during transformation?
B.subtilis
What is the approximate frequency of successful transformation in cells shifted from 42°C to 37°C?
1 in 1,000 cells
What is the minimum size of DNA that can bind and transform cells in Streptococcus and Bacillus?
500 bp
Which protein forms a channel across the outer membrane in N.gonorrhoeae during DNA uptake?
PilQ
What is the function of ComEA protein in B.subtilis?
Binds dsDNA reversibly via ionic interactions
What is the role of ComG during transformation in Streptococcus and Bacillus?
Provides access to ComEA
What are the proteins involved in the surface exclusion of F plasmids during conjugation in bacteria?
traS, traT
Which type of replication process initiates after the single-stranded DNA of the F plasmid is transferred to the recipient cell through the conjugal bridge?
Rolling circle replication
During conjugation, what is the main function of the F pili synthesized by the F factor?
To transfer the single-stranded DNA to the recipient cell
Which gene is responsible for the positive regulation of the F plasmid during conjugation?
traJ
What is a characteristic of Integrative and Conjugative Elements (ICEs)?
They contain genes for integration into the chromosome and encoding conjugation machinery
Which of the following is NOT a requirement for conjugation to occur?
The ability of the plasmid to integrate into the recipient chromosome
What is the specificity of the fertility inheritance cross F- x F-, in terms of the transfer of genetic material?
Sterility and the inability to transfer genetic material
Which process is critical for the stabilization of mating pairs during conjugation in bacteria?
Stabilization by traN and traG
What is a characteristic of F- strains of E. coli?
They can act as recipients in conjugation
What is the main difference between conjugation in E. coli and conjugation in Streptococcus faecalis?
The mechanism of conjugation is understood in E. coli but not in Streptococcus faecalis
What is the function of the genes encoding conjugation machinery in ICEs?
To transfer genetic material between bacteria
Which of the following bacteria is NOT capable of conjugation?
Pseudomonas
What is the function of the surface endonuclease NucA during DNA transformation in Streptococcus and Bacillus?
To create double-stranded breaks
What is the role of ComFA protein during DNA transformation in Streptococcus and Bacillus?
It helps in moving single-stranded DNA into the cell
Which type of recombination occurs between the imported DNA and the bacterial chromosome during DNA transformation in Streptococcus and Bacillus?
Non-reciprocal recombination
What is the role of RecA during DNA transformation in Streptococcus and Bacillus?
It coats single-stranded DNA molecules
Which protein is responsible for converting single-stranded DNA to double-stranded DNA during transformation in Streptococcus and Bacillus?
No such protein is involved
What happens to DNA molecules during the eclipse phase of transformation in Streptococcus and Bacillus?
Double-stranded DNA molecules are converted to single-stranded DNA molecules
Learn about transformation mechanism, plasmid features, the discovery of the F plasmid, genetic organization of F plasmid, and the role of gene products in bacterial conjugation. Join Associate Professor Charlene Kahler at QEII Medical Center for this fascinating lecture.
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