MICR3330 Lateral Gene Transfer: Transformation, Plasmids, and Conjugation
30 Questions
0 Views

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

How are F plasmids involved in bacterial conjugation?

  • They are only able to exchange genetic material via transformation
  • They encode gene products that play a role in transferring genetic material from one bacterium to another (correct)
  • They are responsible for the genetic organization of the chromosome
  • They are only found in Hfr bacterial cells
  • What is the significance of Hfr bacterial cells in chromosomal mapping?

  • They are only involved in transformation
  • They cannot transfer genetic material between cells
  • They are used to create F plasmids
  • They allow the analysis of gene transfer direction and frequency (correct)
  • How are F primes different from F plasmids?

  • F primes play no role in bacterial conjugation
  • F primes cannot complement chromosomal mutations
  • F primes are only able to transfer DNA through transformation
  • F primes can complement chromosomal mutations and aid in gene transfer between bacteria (correct)
  • What is the primary function of the gene products encoded by the F plasmid in bacterial conjugation?

    <p>Facilitating the transfer of genetic material between bacteria</p> Signup and view all the answers

    How do bottlenecks impact genetic diversity in bacterial populations?

    <p>Limit genetic diversity by reducing the number of genetic variants in a population</p> Signup and view all the answers

    Why is chromosomal mapping important in understanding bacterial genetics?

    <p>To establish the order and distance between genes on a chromosome</p> Signup and view all the answers

    Which bacteria requires ComQ proteins for DNA binding during transformation?

    <p>B.subtilis</p> Signup and view all the answers

    What is the approximate frequency of successful transformation in cells shifted from 42°C to 37°C?

    <p>1 in 1,000 cells</p> Signup and view all the answers

    What is the minimum size of DNA that can bind and transform cells in Streptococcus and Bacillus?

    <p>500 bp</p> Signup and view all the answers

    Which protein forms a channel across the outer membrane in N.gonorrhoeae during DNA uptake?

    <p>PilQ</p> Signup and view all the answers

    What is the function of ComEA protein in B.subtilis?

    <p>Binds dsDNA reversibly via ionic interactions</p> Signup and view all the answers

    What is the role of ComG during transformation in Streptococcus and Bacillus?

    <p>Provides access to ComEA</p> Signup and view all the answers

    What are the proteins involved in the surface exclusion of F plasmids during conjugation in bacteria?

    <p>traS, traT</p> Signup and view all the answers

    Which type of replication process initiates after the single-stranded DNA of the F plasmid is transferred to the recipient cell through the conjugal bridge?

    <p>Rolling circle replication</p> Signup and view all the answers

    During conjugation, what is the main function of the F pili synthesized by the F factor?

    <p>To transfer the single-stranded DNA to the recipient cell</p> Signup and view all the answers

    Which gene is responsible for the positive regulation of the F plasmid during conjugation?

    <p>traJ</p> Signup and view all the answers

    What is a characteristic of Integrative and Conjugative Elements (ICEs)?

    <p>They contain genes for integration into the chromosome and encoding conjugation machinery</p> Signup and view all the answers

    Which of the following is NOT a requirement for conjugation to occur?

    <p>The ability of the plasmid to integrate into the recipient chromosome</p> Signup and view all the answers

    What is the specificity of the fertility inheritance cross F- x F-, in terms of the transfer of genetic material?

    <p>Sterility and the inability to transfer genetic material</p> Signup and view all the answers

    Which process is critical for the stabilization of mating pairs during conjugation in bacteria?

    <p>Stabilization by traN and traG</p> Signup and view all the answers

    What is a characteristic of F- strains of E. coli?

    <p>They can act as recipients in conjugation</p> Signup and view all the answers

    What is the main difference between conjugation in E. coli and conjugation in Streptococcus faecalis?

    <p>The mechanism of conjugation is understood in E. coli but not in Streptococcus faecalis</p> Signup and view all the answers

    What is the function of the genes encoding conjugation machinery in ICEs?

    <p>To transfer genetic material between bacteria</p> Signup and view all the answers

    Which of the following bacteria is NOT capable of conjugation?

    <p>Pseudomonas</p> Signup and view all the answers

    What is the function of the surface endonuclease NucA during DNA transformation in Streptococcus and Bacillus?

    <p>To create double-stranded breaks</p> Signup and view all the answers

    What is the role of ComFA protein during DNA transformation in Streptococcus and Bacillus?

    <p>It helps in moving single-stranded DNA into the cell</p> Signup and view all the answers

    Which type of recombination occurs between the imported DNA and the bacterial chromosome during DNA transformation in Streptococcus and Bacillus?

    <p>Non-reciprocal recombination</p> Signup and view all the answers

    What is the role of RecA during DNA transformation in Streptococcus and Bacillus?

    <p>It coats single-stranded DNA molecules</p> Signup and view all the answers

    Which protein is responsible for converting single-stranded DNA to double-stranded DNA during transformation in Streptococcus and Bacillus?

    <p>No such protein is involved</p> Signup and view all the answers

    What happens to DNA molecules during the eclipse phase of transformation in Streptococcus and Bacillus?

    <p>Double-stranded DNA molecules are converted to single-stranded DNA molecules</p> Signup and view all the answers

    More Like This

    MICR3330: Lateral Gene Transfer Lecture
    29 questions
    Use Quizgecko on...
    Browser
    Browser