Podcast
Questions and Answers
What percentage of cells underwent induced competence when shifted from 42°C to 37°C?
What percentage of cells underwent induced competence when shifted from 42°C to 37°C?
- 8%
- 12%
- 15% (correct)
- 5%
What is the frequency of successful transformation mentioned in the text?
What is the frequency of successful transformation mentioned in the text?
- 10^-2
- 10^-4
- 10^-3 (correct)
- 10^-1
Which protein is responsible for moving DNA across the cell wall in the DNA uptake machinery of N. gonorrhoeae (Gram negative)?
Which protein is responsible for moving DNA across the cell wall in the DNA uptake machinery of N. gonorrhoeae (Gram negative)?
- ComE
- PilE
- ComA
- PilQ (correct)
What is the minimum size of DNA that can bind and transform in Streptococcus and Bacillus (Gram positives)?
What is the minimum size of DNA that can bind and transform in Streptococcus and Bacillus (Gram positives)?
Which transformation machinery is required to take up free DNA through the cell wall and membrane(s)?
Which transformation machinery is required to take up free DNA through the cell wall and membrane(s)?
What happens after DNA bound to the surface of a cell is endonuclease nicks DNA according to the text?
What happens after DNA bound to the surface of a cell is endonuclease nicks DNA according to the text?
What is the main objective of the lecture on lateral gene transfer?
What is the main objective of the lecture on lateral gene transfer?
How do F primes differ from F plasmids?
How do F primes differ from F plasmids?
Why are bottlenecks important in the context of genetic diversity?
Why are bottlenecks important in the context of genetic diversity?
In bacterial conjugation, what is the role of the gene products encoded by the F plasmid?
In bacterial conjugation, what is the role of the gene products encoded by the F plasmid?
How can Hfr bacterial cells be utilized in chromosomal mapping?
How can Hfr bacterial cells be utilized in chromosomal mapping?
What does the schematic representation of bottlenecks in the genetic diversity of bacteria illustrate?
What does the schematic representation of bottlenecks in the genetic diversity of bacteria illustrate?
What percentage of bacterial species exhibit natural competence for transformation?
What percentage of bacterial species exhibit natural competence for transformation?
Which of the following statements about transformation is TRUE?
Which of the following statements about transformation is TRUE?
What is the term for the ability of a bacterial cell to bind and take up DNA?
What is the term for the ability of a bacterial cell to bind and take up DNA?
What is the primary difference between transformation and transduction?
What is the primary difference between transformation and transduction?
Which of the following genera is NOT typically competent for transformation?
Which of the following genera is NOT typically competent for transformation?
What is the term for the process by which regions of homology align between the incoming DNA and the host DNA?
What is the term for the process by which regions of homology align between the incoming DNA and the host DNA?
Which gene is responsible for the synthesis of F pili in F plasmids?
Which gene is responsible for the synthesis of F pili in F plasmids?
Which protein is a positive regulator of F plasmid functions?
Which protein is a positive regulator of F plasmid functions?
In which step of conjugation does a bacterium with the F pilus contact the recipient cell (F-)?
In which step of conjugation does a bacterium with the F pilus contact the recipient cell (F-)?
What is the purpose of the finO gene in F plasmid conjugation?
What is the purpose of the finO gene in F plasmid conjugation?
Which stage of conjugation involves the initiation of rolling circle replication of the F plasmid?
Which stage of conjugation involves the initiation of rolling circle replication of the F plasmid?
What is the role of the transferred ssDNA in the recipient cell during conjugation?
What is the role of the transferred ssDNA in the recipient cell during conjugation?
What is the primary function of the accessory proteins TraM, TraD, and TraY during conjugation?
What is the primary function of the accessory proteins TraM, TraD, and TraY during conjugation?
Which protein remains covalently attached to the 5' phosphate group during the initiation of rolling circle replication?
Which protein remains covalently attached to the 5' phosphate group during the initiation of rolling circle replication?
What factors contribute to the mating aggregate formation and stabilization during conjugation?
What factors contribute to the mating aggregate formation and stabilization during conjugation?
What causes the retraction of the F pilus during conjugation?
What causes the retraction of the F pilus during conjugation?
What triggers the initiation of rolling circle replication during conjugation?
What triggers the initiation of rolling circle replication during conjugation?
