Podcast
Questions and Answers
What does a blue color change indicate in a fecal occult blood test?
What does a blue color change indicate in a fecal occult blood test?
What is a key advantage of the fecal occult blood test?
What is a key advantage of the fecal occult blood test?
In a pregnancy test, when do hCG levels typically become detectable?
In a pregnancy test, when do hCG levels typically become detectable?
Which of the following conditions can a positive fecal occult blood test indicate?
Which of the following conditions can a positive fecal occult blood test indicate?
Signup and view all the answers
What is required for urine specimens used in pregnancy tests?
What is required for urine specimens used in pregnancy tests?
Signup and view all the answers
Which condition is NOT associated with low chloride levels in the body?
Which condition is NOT associated with low chloride levels in the body?
Signup and view all the answers
What does a positive result in a urine chloride test indicate?
What does a positive result in a urine chloride test indicate?
Signup and view all the answers
Which of the following materials is NOT required for performing the urine chloride test?
Which of the following materials is NOT required for performing the urine chloride test?
Signup and view all the answers
Which factor is NOT critical when performing a microscopic examination of urine?
Which factor is NOT critical when performing a microscopic examination of urine?
Signup and view all the answers
What is the primary purpose of performing a microscopic examination of urine?
What is the primary purpose of performing a microscopic examination of urine?
Signup and view all the answers
In metabolic alkalosis, what happens to the pH levels in the blood?
In metabolic alkalosis, what happens to the pH levels in the blood?
Signup and view all the answers
What visual result indicates a positive urine chloride test?
What visual result indicates a positive urine chloride test?
Signup and view all the answers
Which renal disorder is least likely to be detected by urine chloride testing?
Which renal disorder is least likely to be detected by urine chloride testing?
Signup and view all the answers
What hormone does the pregnancy test primarily detect?
What hormone does the pregnancy test primarily detect?
Signup and view all the answers
Which body fluids can HCG be detected in aside from urine?
Which body fluids can HCG be detected in aside from urine?
Signup and view all the answers
Which classification does the Latex Agglutination Inhibition Test belong to?
Which classification does the Latex Agglutination Inhibition Test belong to?
Signup and view all the answers
What is the result when HCG is absent in a non-pregnant individual during the Latex Agglutination Inhibition Test?
What is the result when HCG is absent in a non-pregnant individual during the Latex Agglutination Inhibition Test?
Signup and view all the answers
What is a primary purpose of pregnancy testing?
What is a primary purpose of pregnancy testing?
Signup and view all the answers
What is a key component of the reagents used in the Latex Agglutination Inhibition Test?
What is a key component of the reagents used in the Latex Agglutination Inhibition Test?
Signup and view all the answers
Which test uses immature female white mice?
Which test uses immature female white mice?
Signup and view all the answers
What happens when HCG is present in the sample during the Latex Agglutination Inhibition Test?
What happens when HCG is present in the sample during the Latex Agglutination Inhibition Test?
Signup and view all the answers
What is the minimum volume of urine that must remain in the test tube after decantation?
What is the minimum volume of urine that must remain in the test tube after decantation?
Signup and view all the answers
What is the first procedure step in microscopic urinalysis after placing the urine sample in a test tube?
What is the first procedure step in microscopic urinalysis after placing the urine sample in a test tube?
Signup and view all the answers
Which type of cell is NOT typically expected to be observed in a normal urine microscopic examination?
Which type of cell is NOT typically expected to be observed in a normal urine microscopic examination?
Signup and view all the answers
What is the purpose of resuspending the sediment by agitation after centrifugation?
What is the purpose of resuspending the sediment by agitation after centrifugation?
Signup and view all the answers
Which urinary component would indicate a potential renal disease when found in abnormal amounts?
Which urinary component would indicate a potential renal disease when found in abnormal amounts?
Signup and view all the answers
During microscopic examination, what does a high count of White Blood Cells typically suggest?
During microscopic examination, what does a high count of White Blood Cells typically suggest?
Signup and view all the answers
Which of the following parameters is NOT analyzed using a urinary reagent strip?
Which of the following parameters is NOT analyzed using a urinary reagent strip?
Signup and view all the answers
What kind of urine sample should be used for microscopic examination?
What kind of urine sample should be used for microscopic examination?
Signup and view all the answers
What is the reference range for calcium in plasma concentration?
What is the reference range for calcium in plasma concentration?
Signup and view all the answers
Which type of calcium comprises the largest percentage of circulating calcium in the body?
Which type of calcium comprises the largest percentage of circulating calcium in the body?
Signup and view all the answers
What condition is characterized by an increase in total plasma calcium concentration?
What condition is characterized by an increase in total plasma calcium concentration?
Signup and view all the answers
Which factor does NOT affect calcium absorption in the body?
Which factor does NOT affect calcium absorption in the body?
Signup and view all the answers
What symptoms are associated with hypocalcemia?
What symptoms are associated with hypocalcemia?
Signup and view all the answers
What does a heavy or milky turbidity indicate in a Sulkowitch test?
What does a heavy or milky turbidity indicate in a Sulkowitch test?
Signup and view all the answers
In the context of metabolic alkalosis, an increased urine chloride level indicates which type?
In the context of metabolic alkalosis, an increased urine chloride level indicates which type?
Signup and view all the answers
During a urine chloride test, what reagent is used to precipitate chloride as silver chloride?
During a urine chloride test, what reagent is used to precipitate chloride as silver chloride?
Signup and view all the answers
What is the primary purpose of the Sulkowitch test?
What is the primary purpose of the Sulkowitch test?
Signup and view all the answers
Which reagent is NOT included in the composition of the Sulkowitch reagent?
Which reagent is NOT included in the composition of the Sulkowitch reagent?
Signup and view all the answers
What does a positive result in the Sulkowitch test indicate?
What does a positive result in the Sulkowitch test indicate?
Signup and view all the answers
Which of the following is an indicator of heavy turbidity in the Sulkowitch test?
Which of the following is an indicator of heavy turbidity in the Sulkowitch test?
Signup and view all the answers
What can lead to elevated chloride levels in the urine?
What can lead to elevated chloride levels in the urine?
Signup and view all the answers
Which electrolyte is most commonly associated with chloride in the body?
Which electrolyte is most commonly associated with chloride in the body?
Signup and view all the answers
For which condition could a chloride test potentially indicate a problem?
For which condition could a chloride test potentially indicate a problem?
Signup and view all the answers
Which method of collection can be used for a chloride test?
Which method of collection can be used for a chloride test?
Signup and view all the answers
Flashcards
Fecal Occult Blood Test (FOBT)
Fecal Occult Blood Test (FOBT)
A test that detects hidden blood in stool. It can be used to screen for colorectal issues like colon cancer.
FOBT Advantages
FOBT Advantages
Non-invasive (no surgery) and relatively cheap.
FOBT Disadvantages
FOBT Disadvantages
Only detects blood, not the cause. Can have false positive or negative results.
Pregnancy Test
Pregnancy Test
Signup and view all the flashcards
Why 1st Morning Urine for Pregnancy Tests?
Why 1st Morning Urine for Pregnancy Tests?
Signup and view all the flashcards
Calcium in the Body
Calcium in the Body
Signup and view all the flashcards
Reference Range for Calcium
Reference Range for Calcium
Signup and view all the flashcards
Factors Affecting Calcium Absorption
Factors Affecting Calcium Absorption
Signup and view all the flashcards
Ionized Calcium
Ionized Calcium
Signup and view all the flashcards
Complexed Calcium
Complexed Calcium
Signup and view all the flashcards
Protein-Bound Calcium
Protein-Bound Calcium
Signup and view all the flashcards
Hypocalcemia
Hypocalcemia
Signup and view all the flashcards
Hypercalcemia
Hypercalcemia
Signup and view all the flashcards
Sulkowitch Test
Sulkowitch Test
Signup and view all the flashcards
Positive Sulkowitch Test
Positive Sulkowitch Test
Signup and view all the flashcards
Negative Sulkowitch Test
Negative Sulkowitch Test
Signup and view all the flashcards
Chloride Test
Chloride Test
Signup and view all the flashcards
High Chloride Levels
High Chloride Levels
Signup and view all the flashcards
Electrolyte
Electrolyte
Signup and view all the flashcards
Fluid Balance
Fluid Balance
Signup and view all the flashcards
Blood Pressure
Blood Pressure
Signup and view all the flashcards
Low Urine Chloride
Low Urine Chloride
Signup and view all the flashcards
Urine Chloride Test
Urine Chloride Test
Signup and view all the flashcards
Microscopic Urine Examination
Microscopic Urine Examination
Signup and view all the flashcards
Purpose of Microscopic Urine Examination
Purpose of Microscopic Urine Examination
Signup and view all the flashcards
Factors Influencing Microscopic Examination
Factors Influencing Microscopic Examination
Signup and view all the flashcards
Correlation in Urinalysis
Correlation in Urinalysis
Signup and view all the flashcards
Why is microscopic examination important?
Why is microscopic examination important?
Signup and view all the flashcards
What parameters are important for a valid microscopic examination?
What parameters are important for a valid microscopic examination?
Signup and view all the flashcards
Microscopic Urinalysis
Microscopic Urinalysis
Signup and view all the flashcards
Urine Sediment
Urine Sediment
Signup and view all the flashcards
Red Blood Cells in Urine
Red Blood Cells in Urine
Signup and view all the flashcards
White Blood Cells in Urine
White Blood Cells in Urine
Signup and view all the flashcards
Epithelial Cells in Urine
Epithelial Cells in Urine
Signup and view all the flashcards
Casts in Urine
Casts in Urine
Signup and view all the flashcards
Crystals in Urine
Crystals in Urine
Signup and view all the flashcards
Bacteria in Urine
Bacteria in Urine
Signup and view all the flashcards
HCG
HCG
Signup and view all the flashcards
Pregnancy Test Types
Pregnancy Test Types
Signup and view all the flashcards
Latex Agglutination Inhibition Test
Latex Agglutination Inhibition Test
Signup and view all the flashcards
Positive Pregnancy Test
Positive Pregnancy Test
Signup and view all the flashcards
Negative Pregnancy Test
Negative Pregnancy Test
Signup and view all the flashcards
Why is HCG important?
Why is HCG important?
Signup and view all the flashcards
What are the reagents used in Latex Agglutination Inhibition Test?
What are the reagents used in Latex Agglutination Inhibition Test?
Signup and view all the flashcards
How is the Latex Agglutination Inhibition Test conducted?
How is the Latex Agglutination Inhibition Test conducted?
Signup and view all the flashcards
Study Notes
Urine Calcium Determination
- Sample of Choice: A 24-hour urine collection provides a comprehensive measure of ion levels over a full day, aiding in diagnosing and monitoring health conditions.
- 24-Hour Collection Advantages: Accounts for daily fluctuations in urine output and concentration, giving a complete picture of excretion rates.
- Colorimetric Method (Calcium Arsenazo III): This method uses Arsenazo III reagent to form a blue-colored complex with calcium ions, the color intensity proportional to calcium concentration.
- Atomic Absorption Spectroscopy (AAS): Measures light absorption by calcium atoms in a sample. Highly accurate, detects low calcium levels.
- Ion-Selective Electrode (ISE) Method: Uses an electrode selective to calcium ions. The measured potential difference is proportional to calcium concentration.
- Complexometric Titration (EDTA Titration): EDTA (ethylenediaminetetraacetic acid) forms a complex with calcium ions. The amount of EDTA is measured to determine the concentration.
- Sulkowitch Reagent Method: This method adds Sulkowitch reagent to a urine sample. The reaction creates a precipitate, and the amount of precipitate is measured to determine calcium levels. The reagent is composed of oxalic acid, ammonium oxalate, glacial acetic acid, and distilled water. A milky precipitate of calcium oxalate indicates the presence of calcium.
Urine Chloride Determination
- Methods: Volhard-Harvey Method, Mohr's Method, Ion-Selective Electrode Method.
- Volhard-Harvey Method: Involves a titration process using silver nitrate and ammonium thiocyanate. Add a measured excess of silver nitrate to the urine sample. Filter to remove silver chloride precipitate and titrate the excess silver nitrate with ammonium thiocyanate using ferric ammonium sulfate as an indicator.
- Mohr's Method: Uses mercurimetric titration with silver nitrate and potassium chromate as an indicator. Dissolve the urine sample in distilled water and add potassium chromate. Slowly add silver nitrate until a red-brown precipitate of silver chromate forms.
- Ion-Selective Electrode Method: Uses an ion-selective electrode to directly measure chloride concentration.
Calcium and Chloride Determination: AUBF Laboratory
- Calcium in the Body: Calcium (Ca) is the most abundant mineral in the body, primarily found in bones (99%).
- Calcium Types: Ionized, protein-bound, and complexed.
- Calcium Reference Range: 8.8 - 10.4 mg/dL (2.2 - 2.6 mmol/L).
- Factors Affecting Calcium Absorption: Acidic pH, vitamin D, parathyroid hormone (PTH), high protein diet.
- Calcium Distribution: 50% ionized/free (biologically active), 40% protein bound (biologically inactive), and 10% complexed.
Fecal Occult Blood Test (FOBT)
- Purpose: Non-invasive test to detect hidden blood in stool, often used to screen for colorectal disease (e.g., colon cancer).
- Types: Guaiac smear test and flushable reagent pads.
- Positive Result Interpretation: May indicate colon polyps, colon cancer, esophagitis, gastritis, GI trauma, hemorrhoids, fissures, peptic ulcers, or complications from recent GI surgery.
- Procedure: Collect consecutive stool samples. Smear the stool on a paper card. Add testing solution to the opposite side of the card. A blue color change indicates a positive reaction (hidden blood).
Pregnancy Test
- Principle: Based on the detection of human chorionic gonadotropin (hCG) produced by the placenta after implantation. hCG levels rise during pregnancy.
- Urine Test: Urine specimen collected without preservatives; first morning urine usually has highest hCG concentration.
- Blood Test: Quantitative or qualitative hCG blood tests are available.
- Methods: Inhibition Agglutination Method, Immunochromatographic Assay , Digital Midstream Device, Electrochemiluminescence (ECL) technology, Enzyme-Linked Immunosorbent Assay (ELISA).
- Test By Strips or Cassettes: Rapid immunoassay. Immerse test strip in urine, observe if colored lines appear within 5 minutes.
Studying That Suits You
Use AI to generate personalized quizzes and flashcards to suit your learning preferences.
Related Documents
Description
Test your knowledge on various medical tests, including fecal occult blood tests and pregnancy tests. This quiz covers indicators, advantages, and requirements for accurate results. Learn important facts about how these tests function and what their results can imply.