Medical Microbiology - Microbial Growth

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Questions and Answers

What is the primary factor that dictates the classification of bacteria as psychrophiles, mesophiles, or thermophiles?

  • The pH level most conducive to their survival
  • The range of temperatures in which they exhibit growth (correct)
  • Their ability to produce endospores under harsh conditions
  • Their need for oxygen during cellular respiration

Which temperature is most likely to inhibit the growth of a mesophile?

  • 45°C
  • 37°C
  • 5°C (correct)
  • 25°C

A bacterium found thriving in a hot spring at 70°C would most likely be classified as a:

  • Mesophile
  • Thermophile (correct)
  • Psychrophile
  • Psychrotroph

The term 'psychrotroph' refers to bacteria that:

<p>Can grow at both low and moderate temperatures (C)</p> Signup and view all the answers

A bacterial species with a minimum growth temperature close to 0°C and an optimum near 12°C is MOST accurately classified as a:

<p>Psychrophile (B)</p> Signup and view all the answers

Which of the following is correct regarding the growth range of bacteria?

<p>Growth can only occur between the minimum and maximum range. (C)</p> Signup and view all the answers

Most human pathogens are classified as:

<p>Mesophiles (C)</p> Signup and view all the answers

A bacterium with an optimum growth temperature of 90°C would be categorized as a:

<p>Hyperthermophile (D)</p> Signup and view all the answers

If a bacteria has a maximum growth temperature of 48°C, which of the following is true?

<p>It will grow slowly at 48°C but not above. (C)</p> Signup and view all the answers

What would most accurately describe the temperature range for the majority of bacteria that cause disease in humans?

<p>Narrow range between 10 to 48°C (B)</p> Signup and view all the answers

Which enzyme is responsible for converting superoxide ($O_2^-$) into hydrogen peroxide ($H_2O_2$) and oxygen ($O_2$)?

<p>Superoxide dismutase (SOD) (C)</p> Signup and view all the answers

An organism that thrives in an environment with 21% oxygen and has both SOD and catalase is classified as:

<p>Obligate aerobe (A)</p> Signup and view all the answers

A bacterium that can grow in both the presence and absence of oxygen, and possesses both SOD and catalase, is best described as a:

<p>Facultative anaerobe (A)</p> Signup and view all the answers

An aerotolerant anaerobe possesses which of the following characteristics?

<p>Does not use oxygen but has SOD which removes toxic forms of oxygen (A)</p> Signup and view all the answers

Which of the following best describes a microaerophile?

<p>Requires low levels of oxygen (5%) for growth. (A)</p> Signup and view all the answers

An obligate anaerobe is characterized by its inability to grow in the presence of oxygen, and it lacks:

<p>Both catalase and SOD (D)</p> Signup and view all the answers

Which of these organisms is expected to grow throughout a thioglycolate broth?

<p>Facultative anaerobe (B)</p> Signup and view all the answers

What is the function of thioglycolate in thioglycolate broth?

<p>To create an anaerobic environment by reducing oxygen. (A)</p> Signup and view all the answers

Which term describes bacteria that require elevated carbon dioxide levels for growth?

<p>Capnophiles (A)</p> Signup and view all the answers

Which of the following is NOT a location where Capnophiles are commonly found?

<p>The external skin surface (B)</p> Signup and view all the answers

Which of the following best describes the primary role of ion pumps in microbial cells regarding pH?

<p>To maintain a stable internal pH irrespective of the external pH. (B)</p> Signup and view all the answers

A bacterium is found to thrive in a highly acidic environment with a pH around 3. Which of the following classifications best describes this bacterium?

<p>Acidophile (C)</p> Signup and view all the answers

Which of the following accurately depicts the classification of Vibrio cholerae based on its preferred pH?

<p>Alkalophile, capable of growth at pH 8 to 9. (B)</p> Signup and view all the answers

What does the term 'water activity (aw)' specifically measure concerning microbial growth environments?

<p>The ratio of the vapor pressure of a solution to that of pure water. (B)</p> Signup and view all the answers

If a bacterium is classified as osmotolerant, which of the following best describes its interaction with osmotic pressure?

<p>It can tolerate but doesn't require high osmotic pressure. (B)</p> Signup and view all the answers

What is the primary distinction between halophiles and osmotolerant bacteria with respect to salt concentration?

<p>Halophiles require high salt for growth, while osmotolerant bacteria can tolerate it but do not require it. (A)</p> Signup and view all the answers

Which of the following categories of bacteria would likely thrive in an environment with a salt concentration of 25%?

<p>Obligate halophiles (A)</p> Signup and view all the answers

What is the primary reason oxygen is both essential and potentially harmful in microbial growth?

<p>It's required for energy production but produces toxic byproducts. (C)</p> Signup and view all the answers

Which of the following options correctly lists examples of Reactive Oxygen Species (ROS)?

<p>O2-, 1O2, H2O2, OH* (A)</p> Signup and view all the answers

How do microbes typically counteract the damaging effects of Reactive Oxygen Species (ROS)?

<p>By converting ROS into harmless substances with detoxifying enzymes. (A)</p> Signup and view all the answers

Flashcards

Minimum growth temperature

The lowest temperature at which a specific bacteria can grow and survive; below this temperature, growth ceases.

Optimum growth temperature

The temperature at which a particular bacteria exhibits the fastest growth rate and thrives best.

Maximum growth temperature

The highest temperature at which a specific bacteria can survive and grow; beyond this temperature, growth stops and the bacteria may die.

Temperature range for bacterial growth

The range of temperatures within which a bacteria can grow, typically between the minimum and maximum growth temperatures.

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Psychrophiles

Bacteria that thrive in cold temperatures, typically between -10°C and 20°C.

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Psychrotrophs

Bacteria that can tolerate cold temperatures, but grow best at moderate temperatures, typically between 0°C and 30°C.

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Mesophiles

Bacteria that thrive at moderate temperatures, typically between 10°C and 48°C.

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Thermophiles

Bacteria that thrive in hot temperatures, typically between 40°C and 72°C.

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Hyperthermophiles

Bacteria that thrive in extremely high temperatures, typically between 65°C and 110°C.

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Mesophilic bacteria

The group of bacteria that includes most normal human flora and many medically important pathogens. They grow best at 37°C, which is our body temperature.

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Toxic forms of oxygen

Harmful forms of oxygen that cells need to detoxify, including superoxide (O2-) and hydrogen peroxide (H2O2).

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Catalase

An enzyme that breaks down hydrogen peroxide (H2O2) into water (H2O) and oxygen (O2).

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Superoxide Dismutase (SOD)

An enzyme that converts superoxide (O2-) into hydrogen peroxide (H2O2) and oxygen (O2).

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Obligate aerobes

Organisms that require oxygen (O2) for energy production and have both SOD and catalase to detoxify harmful forms of oxygen.

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Facultative anaerobes

Organisms that can grow with or without oxygen (O2) and have both SOD and catalase for detoxifying oxygen.

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Aerotolerant anaerobes

Organisms that can tolerate but do not utilize oxygen (O2). They only have SOD to detoxify oxygen's toxic forms.

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Microaerophiles

Organisms that require low levels of oxygen (O2) for growth. They may have SOD and/or catalase.

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Obligate anaerobes

Organisms that cannot grow in the presence of oxygen (O2). They lack both catalase and SOD.

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Thioglycolate broth

A culture medium that provides both aerobic and anaerobic environments, allowing the growth of different types of organisms.

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Capnophiles

Bacteria that require higher carbon dioxide (CO2) concentrations than normally present in the atmosphere (5-10%).

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pH

The measure of hydrogen ion concentration in a solution, impacting microbial growth. A neutral pH is around 7, while acidic solutions have lower pH values and alkaline solutions have higher pH values.

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Neutrophiles

Microorganisms that thrive in environments with a neutral pH, typically between 6.8 and 7.4. Most bacteria belong to this category.

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Acidophiles

Microorganisms that prefer acidic environments with a pH range of 4.5 to 5.5. Many fungi fall into this category.

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Alkalophiles

Microorganisms that prefer alkaline environments with a pH range of 8 to 9. These are less common than acidophiles or neutrophiles.

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Osmotic Pressure

The pressure exerted by a solution due to the concentration of dissolved solutes, influencing the movement of water across cell membranes.

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Non-halophile

Microorganisms that grow best in environments with low osmotic pressure, similar to their internal environment.

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Osmotolerant/Halotolerant/Barotolerant

Microorganisms that can tolerate high osmotic pressure, but do not require it for growth. They can survive in environments with higher salt concentrations, up to 10%.

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Obligate or Extreme Halophiles/Osmophiles

Microorganisms that require a relatively high salt concentration for growth, usually above 20%. Some Archaea fall into this category.

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Oxygen Requirement for Microbial Growth

The requirement of oxygen for growth, categorized as obligate aerobes, facultative anaerobes, microaerophiles, aerotolerant anaerobes, and obligate anaerobes.

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Reactive Oxygen Species (ROS)

The utilization of oxygen during metabolism can generate harmful reactive oxygen species (ROS or free radicals). These are toxic molecules that can damage cells. However, cells have detoxifying enzymes to convert ROS to harmless substances.

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Study Notes

Medical Microbiology - Microbial Growth and Nutrition

  • Medical microbiology is the study of microorganisms that cause disease in humans.
  • Microorganisms require specific physical, gaseous, and chemical conditions for optimal growth.

Microbial Growth Curve

  • Bacterial growth progresses through distinct phases:
    • Lag phase: initial period of adjustment to new environment, no increase in cell number
    • Log (exponential) phase: rapid cell division; cells multiply at constant rate; measured on log scale, exponential growth
    • Stationary phase: rate of cell division equals rate of cell death, no net change in cell number
    • Death phase: cells die off at a constant rate; logarithmic decline

Bacterial Growth Requirements - Physical

  • Temperature:

    • Minimum growth temperature: the lowest temperature at which bacteria can grow.
    • Optimum growth temperature: the temperature at which bacteria grow most rapidly.
    • Maximum growth temperature: the highest temperature at which bacteria can grow.
    • The temp range is the range between the minimum and maximum temperatures. Growth does not occur outside of this range
  • Classification of Bacteria based on Temperature:

    • Psychrophiles: cold-loving bacteria, optimal growth below 20 °C.
    • Psychrotrophs: cold-tolerant bacteria, grow best between 0 and 30 °C and tolerate cold
    • Mesophiles: moderate-temperature loving bacteria, optimal growth between 10-48 °C; includes most human pathogens (body temperature)
    • Thermophiles: heat-loving bacteria, optimal growth between 40-72 °C
    • Hyperthermophiles: extreme heat-loving bacteria, optimal growth between 65-110 °C
  • pH:

    • Majority of bacteria are neutrophiles and grow best between pH 6.8 - 7.4
    • Acidophiles: grow best at low pH (less than 6.8)
    • Alkalophiles: grow best at high pH (above 7.4)
  • Osmotic Pressure:

    • Osmotolerant/halotolerant/barotolerant bacteria can grow in isotonic or hypotonic environments and can tolerate high osmotic pressure (but do not require it).
    • Obligate or extreme halophiles/osmophiles require relatively high salt concentrations for growth.
  • Water Activity (aw): aw is a measure of water availability; the ratio of the vapor pressure of a solution to the vapor pressure of pure water (always 1.00 for pure water ).

Bacterial Growth requirements - Gaseous

  • Oxygen:
    • Obligate aerobes require oxygen to grow
    • Facultative anaerobes can grow in the presence or absence of oxygen.
    • Aerotolerant anaerobes tolerate oxygen but do not require it for growth
    • Microaerophiles need very low levels of oxygen to grow.
    • Obligate anaerobes cannot grow in the presence of oxygen.
  • Carbon dioxide (COâ‚‚):
    • Most bacteria can grow with low levels of CO2.
    • Capnophiles require high CO2 levels to grow.

Bacterial Growth requirements - Chemical

  • Microbes have different nutritional needs which affect bacterial growth

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