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Questions and Answers
Which of the following individuals is credited with publishing the first manuscript illustrating microbes?
Which of the following individuals is credited with publishing the first manuscript illustrating microbes?
Which of the following individuals is credited with developing the first method of DNA sequencing that was fast enough to sequence large genomes?
Which of the following individuals is credited with developing the first method of DNA sequencing that was fast enough to sequence large genomes?
The theory that microbes could arise spontaneously, without a parental organism, is known as:
The theory that microbes could arise spontaneously, without a parental organism, is known as:
Which of the following individuals is considered the founder of the scientific method of microbiology?
Which of the following individuals is considered the founder of the scientific method of microbiology?
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What is the primary purpose of a Gram stain?
What is the primary purpose of a Gram stain?
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Which statement accurately describes fluorescence microscopy?
Which statement accurately describes fluorescence microscopy?
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In which type of microscopy do electrons pass through the specimen to reveal internal structures?
In which type of microscopy do electrons pass through the specimen to reveal internal structures?
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What distinguishes scanning electron microscopy (SEM) from transmission electron microscopy (TEM)?
What distinguishes scanning electron microscopy (SEM) from transmission electron microscopy (TEM)?
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Which microscopy technique utilizes fluorescent DNA probes?
Which microscopy technique utilizes fluorescent DNA probes?
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What is a characteristic of a pure culture?
What is a characteristic of a pure culture?
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Which scientist is known for the discovery of the antiseptic technique involving handwashing?
Which scientist is known for the discovery of the antiseptic technique involving handwashing?
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What significant contribution did Edward Jenner make to medicine?
What significant contribution did Edward Jenner make to medicine?
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What defines an extremophile?
What defines an extremophile?
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What was Martanus Beijerinck's contribution to microbiology?
What was Martanus Beijerinck's contribution to microbiology?
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What are lithotrophs known for?
What are lithotrophs known for?
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Which term describes organisms that do not share a common ancestor?
Which term describes organisms that do not share a common ancestor?
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Which scientist modified the five kingdom classification system?
Which scientist modified the five kingdom classification system?
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What defines the resolution in microscopy?
What defines the resolution in microscopy?
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Which type of microscopy generates a light image over a dark background?
Which type of microscopy generates a light image over a dark background?
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What is minimum resolution distance calculated by?
What is minimum resolution distance calculated by?
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What is the purpose of fixation in microscopy?
What is the purpose of fixation in microscopy?
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What does numerical aperture (NA) determine in light microscopy?
What does numerical aperture (NA) determine in light microscopy?
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What is an example of a differential stain?
What is an example of a differential stain?
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Which microscopy technique uses light to enhance contrast in transparent specimens?
Which microscopy technique uses light to enhance contrast in transparent specimens?
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What is aberration in microscopy?
What is aberration in microscopy?
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What role does the cell wall play in a bacterial cell?
What role does the cell wall play in a bacterial cell?
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What type of fatty acids increase membrane fluidity at low temperatures?
What type of fatty acids increase membrane fluidity at low temperatures?
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Which statement accurately describes the structure of a phospholipid?
Which statement accurately describes the structure of a phospholipid?
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What is the primary function of the flagellum in bacterial cells?
What is the primary function of the flagellum in bacterial cells?
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What distinguishes archaea from bacteria at the molecular level?
What distinguishes archaea from bacteria at the molecular level?
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What mechanism allows solutes to move against their concentration gradient?
What mechanism allows solutes to move against their concentration gradient?
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Which characteristic of the cell membrane contributes to its semipermeability?
Which characteristic of the cell membrane contributes to its semipermeability?
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What is the function of hopanoids in bacterial membranes?
What is the function of hopanoids in bacterial membranes?
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What is the primary function of the bacterial cell wall?
What is the primary function of the bacterial cell wall?
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How does penicillin affect bacterial cell walls?
How does penicillin affect bacterial cell walls?
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What is the role of Braun's lipoprotein in gram-negative bacteria?
What is the role of Braun's lipoprotein in gram-negative bacteria?
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What is the process called where older cellular components accumulate at one pole during cell division?
What is the process called where older cellular components accumulate at one pole during cell division?
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Which organism exhibits asymmetrical cell division resulting in two distinct daughter cell types?
Which organism exhibits asymmetrical cell division resulting in two distinct daughter cell types?
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What did the Hershey-Chase experiment demonstrate?
What did the Hershey-Chase experiment demonstrate?
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What is the function of topoisomerases in DNA structure?
What is the function of topoisomerases in DNA structure?
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What distinguishes negative supercoiling from positive supercoiling in DNA?
What distinguishes negative supercoiling from positive supercoiling in DNA?
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Which of the following enzymes creates a double-strand break for negative supercoiling?
Which of the following enzymes creates a double-strand break for negative supercoiling?
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What did Avery's experiments establish about DNA?
What did Avery's experiments establish about DNA?
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What is the composition of the bacterial cell wall primarily based on?
What is the composition of the bacterial cell wall primarily based on?
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What structural feature of the DNA double helix allows for base pairing?
What structural feature of the DNA double helix allows for base pairing?
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What is the consequence of osmotic shock in eukaryotic microbes?
What is the consequence of osmotic shock in eukaryotic microbes?
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What is the relationship between nucleotides in DNA?
What is the relationship between nucleotides in DNA?
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Flashcards
Microbe
Microbe
A microscopic organism that may exist as a single cell or in a colony of cells.
Impact of Microbes
Impact of Microbes
Microbes have significantly influenced human history, culture, and health.
Florence Nightingale
Florence Nightingale
Nurse who highlighted the impact of diseases in warfare, especially microbial infections.
Robert Koch
Robert Koch
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Koch's Postulates
Koch's Postulates
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Gram stain
Gram stain
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Acid-fast stain
Acid-fast stain
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Fluorescent microscopy
Fluorescent microscopy
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Transmission electron microscopy (TEM)
Transmission electron microscopy (TEM)
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Cryo-Electron tomography
Cryo-Electron tomography
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Resolution
Resolution
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Magnification
Magnification
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Numerical Aperture (NA)
Numerical Aperture (NA)
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Minimum Resolution Distance (R)
Minimum Resolution Distance (R)
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Aberration
Aberration
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Bright-field Microscopy
Bright-field Microscopy
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Wet Mount
Wet Mount
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Simple Stain
Simple Stain
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Pure culture
Pure culture
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Edward Jenner
Edward Jenner
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Ignaz Semmelweis
Ignaz Semmelweis
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Alexander Fleming
Alexander Fleming
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Extremophiles
Extremophiles
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Endosymbionts
Endosymbionts
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Monophyletic
Monophyletic
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Transformation in bacteria
Transformation in bacteria
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X-ray diffraction analysis
X-ray diffraction analysis
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Cytoplasm
Cytoplasm
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Phospholipid
Phospholipid
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Passive transport
Passive transport
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Active transport
Active transport
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Saturated fatty acids
Saturated fatty acids
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Unsaturated fatty acids
Unsaturated fatty acids
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ATP-binding cassette (ABC)
ATP-binding cassette (ABC)
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Phosphotransferase system (PTS)
Phosphotransferase system (PTS)
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Cell Wall Function
Cell Wall Function
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Peptidoglycan Structure
Peptidoglycan Structure
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Penicillin's Role
Penicillin's Role
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Vancomycin's Role
Vancomycin's Role
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Pseudomurein
Pseudomurein
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Binary Fission
Binary Fission
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Cell Asymmetry
Cell Asymmetry
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Caulobacter crescentus
Caulobacter crescentus
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Flagella Function
Flagella Function
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Chemotaxis
Chemotaxis
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Nucleoid Structure
Nucleoid Structure
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Negative Supercoiling
Negative Supercoiling
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Topoisomerase Type I
Topoisomerase Type I
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Topoisomerase Type II
Topoisomerase Type II
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Study Notes
Introduction and Origin
- Microbes are living organisms needing a microscope to be seen, ranging from 0.2 µm to 5 mm in size.
- There are an estimated 10^12 microbial species, with only a small fraction identified.
- Microbes include bacteria, archaea, and eukarya.
- Fred Sanger developed the first method for DNA sequencing fast enough to sequence large genomes.
Impact of Microbes on Human History
- Major epidemics (e.g., Plague of Justinian, Black Death, Spanish flu) have killed millions, shaped history.
- HIV/AIDS and COVID-19 significantly impacted global health and well-being.
- Florence Nightingale recognized the importance of disease in warfare.
- She developed the scientific method of microbiology, proving the link between microbes and disease.
- She founded the principles of medical statistics and professional nursing.
Origins of Medical Microbiology
- Robert Koch established the scientific method for microbiology, linking microbes to specific diseases.
- He proved the germ theory of disease, showing how microbes cause infections.
- He used anthrax to demonstrate the chain of infection.
- Koch's postulates are used to establish the disease causation link between microbes and infections.
Microbial Ecology and Environment
- Lithotrophs used inorganic materials for their energy.
- Sergei Winogradsky developed enrichment cultures.
- Extremophiles are organisms living in extreme environments.
- Endosymbionts are microbes living symbiotically inside a larger organism.
- Biofilms are slimy layers formed by microbes sticking together in communities.
Cell Biology and the Amazing DNA Revolution
- Frederick Griffith discovered bacterial transformation.
- Oswald Avery discovered DNA was the transforming substance, proving its role in heredity.
Discovery and Observation
- Resolution limits the smallest distance objects can be distinguished
- The human eye can only distinguish about 150 microns
- Microbes vary significantly in size, covering several orders of magnitude.
Optics and Properties of Light
- Electromagnetic radiation is composed of perpendicular electric and magnetic waves.
- Visible light has a wavelength range of 400-750 nm.
- Refraction changes light's direction entering a substance with a higher refractive index.
- Magnification uses the bending of light (refraction) increasing the apparent size of objects.
- Parabolic lenses concentrate or spread light based on the specific shapes.
Cell Biology and Microscopy
- Eukaryotic and prokaryotic microbes have various sizes in the range of micrometers (µm) to nanometers (nm).
- Light microscopy, scanning and transmission electron microscopes, and other advanced techniques are used for observing and studying microorganisms.
- Staining techniques can enhance cell visualization using various dyes and stains.
- Wet mounts are simple preparation methods, while fixation and staining techniques enhance contrast.
- Fluorescence microscopy uses fluorescent materials for detailed studies, allowing specific visualization of cells and structures within them.
Electron Microscopy
- Electron microscopy uses electrons instead of light for high magnification.
- Transmission electron microscopy (TEM) involves the passing of electrons through a specimen.
- Scanning electron microscopy (SEM) involves scanning the surface of a specimen with electrons.
Structure and Function
- The bacterial cell comprises cytoplasm, a cell membrane, and commonly a cell wall
- Cell fractionation separates subcellular components.
- Ultracentrifuges separate particles based on size and density.
- Cell membrane is a semi-permeable phospholipid bi-layer, which controls entry and exit of molecules.
- Unsaturated fatty acids increase membrane fluidity, while saturated ones decrease it.
- Hopanoids stabilize or modify membrane fluidity in various environments.
- Membrane molecules participate in active and passive transport across the membrane.
Genomes and Replication
- The genome represents the complete set of genetic material within a cell.
- DNA consists of a double helix where bases are paired in specific combinations: G with C and A with T.
- Organisms possess a variety of supercoiling for DNA packaging.
- Circular DNA differs from linear DNA in its structure and organization.
- DNA polymerase III is a key enzyme used during replication.
- DNA replication uses one parental strand to synthesize a new complementary strand.
- Replication proceeds in two opposite directions around the chromosome.
- Plasmids are extrachromosomal DNA molecules that replicate independently.
Additional Topics
- Bacterial cell walls differ structurally between Gram-positive and Gram-negative bacteria.
- Bacterial cell walls are made of peptidoglycans, and unique structures contribute to their functions (e.g, virulence and resistance).
- Different types of microscopy are used for observing a variety of microorganisms like bacteria, fungi, algae, and viruses.
- Various microscopy techniques exist, each offering unique capabilities to improve resolutions and visual analyses.
- Eukaryotic microbes have unique internal structures like vacuoles to deal with osmotic stress.
- Prokaryotic microbes like bacteria reproduce via binary fission, resulting in two identical daughter cells.
- DNA replication is semi-conservative (synthesizes a new strand using a template)-
- Processes like transformation, transduction, and conjugation. Discuss how genetic material can be transferred between different bacterial cells in various scenarios.
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Description
Explore the foundational concepts of medical microbiology, including the origins of microbes, their impact on human history, and key figures like Robert Koch and Florence Nightingale. This quiz delves into the crucial relationship between microbes and diseases that have shaped our world.