Medical Microbiology Introduction
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Questions and Answers

Which of the following individuals is credited with publishing the first manuscript illustrating microbes?

  • Louis Pasteur
  • Antonie van Leeuwenhoek
  • Robert Koch
  • Robert Hooke (correct)
  • Which of the following individuals is credited with developing the first method of DNA sequencing that was fast enough to sequence large genomes?

  • Louis Pasteur
  • Fred Sanger (correct)
  • Antonie van Leeuwenhoek
  • Robert Koch
  • The theory that microbes could arise spontaneously, without a parental organism, is known as:

  • Koch's Postulates
  • Miasma Theory
  • Spontaneous Generation (correct)
  • Germ Theory
  • Which of the following individuals is considered the founder of the scientific method of microbiology?

    <p>Robert Koch (C)</p> Signup and view all the answers

    What is the primary purpose of a Gram stain?

    <p>To differentiate between gram-positive and gram-negative bacteria (D)</p> Signup and view all the answers

    Which statement accurately describes fluorescence microscopy?

    <p>It relies on fluorophores that emit light when excited by specific wavelengths. (D)</p> Signup and view all the answers

    In which type of microscopy do electrons pass through the specimen to reveal internal structures?

    <p>Transmission electron microscopy (TEM) (B)</p> Signup and view all the answers

    What distinguishes scanning electron microscopy (SEM) from transmission electron microscopy (TEM)?

    <p>TEM reveals internal structures while SEM reveals external structures. (D)</p> Signup and view all the answers

    Which microscopy technique utilizes fluorescent DNA probes?

    <p>Fluorescence In Situ Hybridization (FISH) (D)</p> Signup and view all the answers

    What is a characteristic of a pure culture?

    <p>Used to prove the cause of a specific disease (D)</p> Signup and view all the answers

    Which scientist is known for the discovery of the antiseptic technique involving handwashing?

    <p>Ignaz Semmelweis (B)</p> Signup and view all the answers

    What significant contribution did Edward Jenner make to medicine?

    <p>Discovered that cowpox protects against smallpox (C)</p> Signup and view all the answers

    What defines an extremophile?

    <p>Microbes that thrive in extreme environments (B)</p> Signup and view all the answers

    What was Martanus Beijerinck's contribution to microbiology?

    <p>He discovered the contagion was not a bacterium but a living fluid (D)</p> Signup and view all the answers

    What are lithotrophs known for?

    <p>Using inorganic materials for biosynthesis (D)</p> Signup and view all the answers

    Which term describes organisms that do not share a common ancestor?

    <p>Polyphyletic (B)</p> Signup and view all the answers

    Which scientist modified the five kingdom classification system?

    <p>Lynn Margulis (A)</p> Signup and view all the answers

    What defines the resolution in microscopy?

    <p>The smallest distance by which two objects can be distinguished (B)</p> Signup and view all the answers

    Which type of microscopy generates a light image over a dark background?

    <p>Dark-field microscopy (D)</p> Signup and view all the answers

    What is minimum resolution distance calculated by?

    <p>The formula λ/2 and Numerical Aperture (NA) (D)</p> Signup and view all the answers

    What is the purpose of fixation in microscopy?

    <p>To adhere cells to a slide in a fixed position (A)</p> Signup and view all the answers

    What does numerical aperture (NA) determine in light microscopy?

    <p>The magnification power of the microscope (A)</p> Signup and view all the answers

    What is an example of a differential stain?

    <p>Gram stain (A)</p> Signup and view all the answers

    Which microscopy technique uses light to enhance contrast in transparent specimens?

    <p>Phase-contrast microscopy (D)</p> Signup and view all the answers

    What is aberration in microscopy?

    <p>A defect in image due to improper light focusing (C)</p> Signup and view all the answers

    What role does the cell wall play in a bacterial cell?

    <p>It covers the cell membrane. (D)</p> Signup and view all the answers

    What type of fatty acids increase membrane fluidity at low temperatures?

    <p>Unsaturated fatty acids (C)</p> Signup and view all the answers

    Which statement accurately describes the structure of a phospholipid?

    <p>It contains a polar hydrophilic head and two hydrophobic tails. (D)</p> Signup and view all the answers

    What is the primary function of the flagellum in bacterial cells?

    <p>To propel the cell. (D)</p> Signup and view all the answers

    What distinguishes archaea from bacteria at the molecular level?

    <p>Archaea have ether linkages in their phospholipids. (B)</p> Signup and view all the answers

    What mechanism allows solutes to move against their concentration gradient?

    <p>Active transport (B)</p> Signup and view all the answers

    Which characteristic of the cell membrane contributes to its semipermeability?

    <p>It is made of a phospholipid bilayer. (C)</p> Signup and view all the answers

    What is the function of hopanoids in bacterial membranes?

    <p>They modify membrane fluidity under stress. (C)</p> Signup and view all the answers

    What is the primary function of the bacterial cell wall?

    <p>Provide rigidity and shape (B)</p> Signup and view all the answers

    How does penicillin affect bacterial cell walls?

    <p>It inhibits the transpeptidase cross-linking peptides (C)</p> Signup and view all the answers

    What is the role of Braun's lipoprotein in gram-negative bacteria?

    <p>Tethers the outer membrane to the peptidoglycan (B)</p> Signup and view all the answers

    What is the process called where older cellular components accumulate at one pole during cell division?

    <p>Polar aging (B)</p> Signup and view all the answers

    Which organism exhibits asymmetrical cell division resulting in two distinct daughter cell types?

    <p>Caulobacter crescentus (A)</p> Signup and view all the answers

    What did the Hershey-Chase experiment demonstrate?

    <p>DNA encodes hereditary information (A)</p> Signup and view all the answers

    What is the function of topoisomerases in DNA structure?

    <p>To introduce or remove supercoils in DNA (A)</p> Signup and view all the answers

    What distinguishes negative supercoiling from positive supercoiling in DNA?

    <p>The direction of the DNA twist (D)</p> Signup and view all the answers

    Which of the following enzymes creates a double-strand break for negative supercoiling?

    <p>Topoisomerase II (D)</p> Signup and view all the answers

    What did Avery's experiments establish about DNA?

    <p>DNA is the transforming substance in bacterial genetics (B)</p> Signup and view all the answers

    What is the composition of the bacterial cell wall primarily based on?

    <p>Peptidoglycan (murein) (B)</p> Signup and view all the answers

    What structural feature of the DNA double helix allows for base pairing?

    <p>Antiparallel strands (B)</p> Signup and view all the answers

    What is the consequence of osmotic shock in eukaryotic microbes?

    <p>Increased internal pressure (C)</p> Signup and view all the answers

    What is the relationship between nucleotides in DNA?

    <p>Linked by phosphodiester bonds (C)</p> Signup and view all the answers

    Flashcards

    Microbe

    A microscopic organism that may exist as a single cell or in a colony of cells.

    Impact of Microbes

    Microbes have significantly influenced human history, culture, and health.

    Florence Nightingale

    Nurse who highlighted the impact of diseases in warfare, especially microbial infections.

    Robert Koch

    Scientist who founded modern microbiology and established Koch's Postulates for identifying pathogens.

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    Koch's Postulates

    A series of criteria to establish a causal relationship between a microbe and an infectious disease.

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    Gram stain

    A method to classify bacteria as gram-positive or gram-negative.

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    Acid-fast stain

    Uses carbolfuchsin to stain Mycobacterium species.

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    Fluorescent microscopy

    Uses fluorophores that emit light to visualize specimens.

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    Transmission electron microscopy (TEM)

    Uses electrons to pass through specimens for internal structure imaging.

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    Cryo-Electron tomography

    Combines images of rapidly frozen samples to create 3D visualizations.

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    Resolution

    The smallest distance two objects can be separated and still distinguished.

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    Magnification

    The act of enlarging the appearance of an object using lenses.

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    Numerical Aperture (NA)

    Determines the resolving power of a lens system in microscopy.

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    Minimum Resolution Distance (R)

    The smallest distance which can be resolved, calculated as λ/2 and NA.

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    Aberration

    A defect in an image caused by light rays not focusing properly.

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    Bright-field Microscopy

    Generates a dark image of an object against a light background.

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    Wet Mount

    A method of viewing live organisms in a drop of water on a slide.

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    Simple Stain

    A staining method that colors cells without affecting surrounding tissue.

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    Pure culture

    A culture containing only one bacterial species, used to establish causation of diseases.

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    Edward Jenner

    Scientist who discovered cowpox vaccination protects against smallpox.

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    Ignaz Semmelweis

    Introduced handwashing with antiseptic to prevent disease transmission in hospitals.

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    Alexander Fleming

    Discovered penicillin, a substance produced by mold that kills bacteria.

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    Extremophiles

    Organisms that thrive in extreme environmental conditions.

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    Endosymbionts

    Microbes that live inside larger organisms, benefiting both parties.

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    Monophyletic

    Groups of organisms that descend from a common ancestor.

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    Transformation in bacteria

    A genetic process where bacteria take up foreign DNA from their environment.

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    X-ray diffraction analysis

    A technique to determine atomic positions in crystallized samples.

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    Cytoplasm

    A viscous gel-like substance within the cell that supports organelles.

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    Phospholipid

    Molecule with hydrophobic tails and a hydrophilic head, forming cell membranes.

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    Passive transport

    Movement of molecules along the concentration gradient without energy.

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    Active transport

    Movement of molecules against the concentration gradient, requiring energy.

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    Saturated fatty acids

    Fatty acids that decrease membrane fluidity, improving function at high temperatures.

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    Unsaturated fatty acids

    Fatty acids that increase membrane fluidity, improving function at low temperatures.

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    ATP-binding cassette (ABC)

    The largest family of active transport systems in cells.

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    Phosphotransferase system (PTS)

    A group translocation mechanism used by bacteria to transport sugars while phosphorylating them.

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    Cell Wall Function

    Provides rigidity and shape to the cell, helping it withstand turgor pressure.

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    Peptidoglycan Structure

    A polymer consisting of sugars and amino acids that forms a mesh-like layer in the bacterial cell wall.

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    Penicillin's Role

    Inhibits transpeptidase, preventing peptide cross-linking in peptidoglycan synthesis.

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    Vancomycin's Role

    Binds to the terminal D-Ala-D-Ala dipeptide, blocking cross-bridge formation in peptidoglycan.

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    Pseudomurein

    A component found in Archaea similar to bacterial peptidoglycan, providing structural support.

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    Binary Fission

    The method of prokaryotic cell division where a single cell divides into two daughter cells.

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    Cell Asymmetry

    Polar aging process where older components localize to one pole during division.

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    Caulobacter crescentus

    A bacterium that generates two types of daughter cells (motile and stalked) due to cell asymmetry.

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    Flagella Function

    A specialized structure used by bacteria for swimming and locomotion.

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    Chemotaxis

    Movement of an organism in response to chemical stimuli, often towards nutrients or away from toxins.

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    Nucleoid Structure

    Compact bacterial chromosome structure formed by supercoiled DNA and loop domains.

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    Negative Supercoiling

    DNA is twisted in the opposite direction of the helix, making it less compact and easier to unwind.

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    Topoisomerase Type I

    An enzyme that relaxes DNA by cutting one strand and reforming it, helping manage supercoiling.

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    Topoisomerase Type II

    Creates double-strand breaks in DNA to introduce negative supercoiling, crucial for replication.

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    Study Notes

    Introduction and Origin

    • Microbes are living organisms needing a microscope to be seen, ranging from 0.2 µm to 5 mm in size.
    • There are an estimated 10^12 microbial species, with only a small fraction identified.
    • Microbes include bacteria, archaea, and eukarya.
    • Fred Sanger developed the first method for DNA sequencing fast enough to sequence large genomes.

    Impact of Microbes on Human History

    • Major epidemics (e.g., Plague of Justinian, Black Death, Spanish flu) have killed millions, shaped history.
    • HIV/AIDS and COVID-19 significantly impacted global health and well-being.
    • Florence Nightingale recognized the importance of disease in warfare.
    • She developed the scientific method of microbiology, proving the link between microbes and disease.
    • She founded the principles of medical statistics and professional nursing.

    Origins of Medical Microbiology

    • Robert Koch established the scientific method for microbiology, linking microbes to specific diseases.
    • He proved the germ theory of disease, showing how microbes cause infections.
    • He used anthrax to demonstrate the chain of infection.
    • Koch's postulates are used to establish the disease causation link between microbes and infections.

    Microbial Ecology and Environment

    • Lithotrophs used inorganic materials for their energy.
    • Sergei Winogradsky developed enrichment cultures.
    • Extremophiles are organisms living in extreme environments.
    • Endosymbionts are microbes living symbiotically inside a larger organism.
    • Biofilms are slimy layers formed by microbes sticking together in communities.

    Cell Biology and the Amazing DNA Revolution

    • Frederick Griffith discovered bacterial transformation.
    • Oswald Avery discovered DNA was the transforming substance, proving its role in heredity.

    Discovery and Observation

    • Resolution limits the smallest distance objects can be distinguished
    • The human eye can only distinguish about 150 microns
    • Microbes vary significantly in size, covering several orders of magnitude.

    Optics and Properties of Light

    • Electromagnetic radiation is composed of perpendicular electric and magnetic waves.
    • Visible light has a wavelength range of 400-750 nm.
    • Refraction changes light's direction entering a substance with a higher refractive index.
    • Magnification uses the bending of light (refraction) increasing the apparent size of objects.
    • Parabolic lenses concentrate or spread light based on the specific shapes.

    Cell Biology and Microscopy

    • Eukaryotic and prokaryotic microbes have various sizes in the range of micrometers (µm) to nanometers (nm).
    • Light microscopy, scanning and transmission electron microscopes, and other advanced techniques are used for observing and studying microorganisms.
    • Staining techniques can enhance cell visualization using various dyes and stains.
    • Wet mounts are simple preparation methods, while fixation and staining techniques enhance contrast.
    • Fluorescence microscopy uses fluorescent materials for detailed studies, allowing specific visualization of cells and structures within them.

    Electron Microscopy

    • Electron microscopy uses electrons instead of light for high magnification.
    • Transmission electron microscopy (TEM) involves the passing of electrons through a specimen.
    • Scanning electron microscopy (SEM) involves scanning the surface of a specimen with electrons.

    Structure and Function

    • The bacterial cell comprises cytoplasm, a cell membrane, and commonly a cell wall
    • Cell fractionation separates subcellular components.
    • Ultracentrifuges separate particles based on size and density.
    • Cell membrane is a semi-permeable phospholipid bi-layer, which controls entry and exit of molecules.
    • Unsaturated fatty acids increase membrane fluidity, while saturated ones decrease it.
    • Hopanoids stabilize or modify membrane fluidity in various environments.
    • Membrane molecules participate in active and passive transport across the membrane.

    Genomes and Replication

    • The genome represents the complete set of genetic material within a cell.
    • DNA consists of a double helix where bases are paired in specific combinations: G with C and A with T.
    • Organisms possess a variety of supercoiling for DNA packaging.
    • Circular DNA differs from linear DNA in its structure and organization.
    • DNA polymerase III is a key enzyme used during replication.
    • DNA replication uses one parental strand to synthesize a new complementary strand.
    • Replication proceeds in two opposite directions around the chromosome.
    • Plasmids are extrachromosomal DNA molecules that replicate independently.

    Additional Topics

    • Bacterial cell walls differ structurally between Gram-positive and Gram-negative bacteria.
    • Bacterial cell walls are made of peptidoglycans, and unique structures contribute to their functions (e.g, virulence and resistance).
    • Different types of microscopy are used for observing a variety of microorganisms like bacteria, fungi, algae, and viruses.
    • Various microscopy techniques exist, each offering unique capabilities to improve resolutions and visual analyses.
    • Eukaryotic microbes have unique internal structures like vacuoles to deal with osmotic stress.
    • Prokaryotic microbes like bacteria reproduce via binary fission, resulting in two identical daughter cells.
    • DNA replication is semi-conservative (synthesizes a new strand using a template)-
    • Processes like transformation, transduction, and conjugation. Discuss how genetic material can be transferred between different bacterial cells in various scenarios.

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    Description

    Explore the foundational concepts of medical microbiology, including the origins of microbes, their impact on human history, and key figures like Robert Koch and Florence Nightingale. This quiz delves into the crucial relationship between microbes and diseases that have shaped our world.

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