Podcast
Questions and Answers
What is the purpose of using fixatives like formalin and glutaraldehyde in tissue preparation?
What is the purpose of using fixatives like formalin and glutaraldehyde in tissue preparation?
- To enhance the staining properties of the tissues
- To preserve and stabilize cellular structures (correct)
- To reduce the size of the tissue samples
- To increase the visibility of extracellular materials
Which of the following is a commonly used embedding material for light microscopy?
Which of the following is a commonly used embedding material for light microscopy?
- Paraffin (correct)
- Plastic resins
- Glutaraldehyde
- Osmium tetroxide
What role does osmium tetroxide play in the preparation of tissue samples?
What role does osmium tetroxide play in the preparation of tissue samples?
- It is used to enhance the color of extracellular materials
- It acts as a fixing agent for proteins only
- It stains cellular lipids and proteins for examination (correct)
- It provides structural consistency to the tissues
What is the main reason for staining tissue sections before microscopic examination?
What is the main reason for staining tissue sections before microscopic examination?
What characteristic of the embedding materials used for fixed tissues is highlighted in the content?
What characteristic of the embedding materials used for fixed tissues is highlighted in the content?
What is the primary purpose of using heavy metal compounds in tissue preparation for microscopy?
What is the primary purpose of using heavy metal compounds in tissue preparation for microscopy?
In scanning electron microscopy (SEM), how are areas that reflect electrons represented in the final image?
In scanning electron microscopy (SEM), how are areas that reflect electrons represented in the final image?
Why are SEM images considered easy to interpret?
Why are SEM images considered easy to interpret?
What type of resources does AccessMedicine include?
What type of resources does AccessMedicine include?
What role do the reflected electrons play in electron microscopy?
What role do the reflected electrons play in electron microscopy?
Which of the following compounds is NOT commonly used to improve contrast in Transmission Electron Microscopy (TEM)?
Which of the following compounds is NOT commonly used to improve contrast in Transmission Electron Microscopy (TEM)?
What has replaced the electron and light micrographs in the book?
What has replaced the electron and light micrographs in the book?
What characteristic do areas of tissue that were absorbed or deflected electrons exhibit in SEM imaging?
What characteristic do areas of tissue that were absorbed or deflected electrons exhibit in SEM imaging?
Which feature of Junqueira’s Basic Histology is emphasized as valuable?
Which feature of Junqueira’s Basic Histology is emphasized as valuable?
What is the first step in preparing tissue for sectioning?
What is the first step in preparing tissue for sectioning?
What is the effect of heavy metal ions on cellular macromolecules during tissue preparation?
What is the effect of heavy metal ions on cellular macromolecules during tissue preparation?
What is the main purpose of the art and figures presented in each chapter?
What is the main purpose of the art and figures presented in each chapter?
What is one method through which users can provide feedback to the author?
What is one method through which users can provide feedback to the author?
Which of the following processes does not occur during the preparation of samples for electron microscopy?
Which of the following processes does not occur during the preparation of samples for electron microscopy?
What is the purpose of infiltrating tissue with melted paraffin?
What is the purpose of infiltrating tissue with melted paraffin?
What are the available formats for the learning aid mentioned?
What are the available formats for the learning aid mentioned?
What feature distinguishes the ultra-microtome used for TEM from a standard microtome?
What feature distinguishes the ultra-microtome used for TEM from a standard microtome?
How are tissues prepared immediately after removal from the body?
How are tissues prepared immediately after removal from the body?
What feature distinguishes Junqueira’s Basic Histology from other medical textbooks?
What feature distinguishes Junqueira’s Basic Histology from other medical textbooks?
What is the target audience for the described educational resources?
What is the target audience for the described educational resources?
What is the purpose of using fixatives during tissue preparation?
What is the purpose of using fixatives during tissue preparation?
What happens to the paraffin sections after they are cut?
What happens to the paraffin sections after they are cut?
Why is vascular perfusion used during the fixation process?
Why is vascular perfusion used during the fixation process?
What thickness of sections is typically prepared for TEM?
What thickness of sections is typically prepared for TEM?
What role do silver bromide crystals play in autoradiography?
What role do silver bromide crystals play in autoradiography?
How does scanning electron microscopy (SEM) differ from transmission electron microscopy (TEM)?
How does scanning electron microscopy (SEM) differ from transmission electron microscopy (TEM)?
What must be done to specimens before they are observed using scanning electron microscopy?
What must be done to specimens before they are observed using scanning electron microscopy?
What type of information can autoradiography provide regarding DNA in tissues?
What type of information can autoradiography provide regarding DNA in tissues?
Which of the following is a key characteristic of scanning electron microscopy?
Which of the following is a key characteristic of scanning electron microscopy?
What happens to silver bromide crystals when exposed to radiation during the autoradiographic process?
What happens to silver bromide crystals when exposed to radiation during the autoradiographic process?
Which observation technique can indicate the presence of radiolabeled macromolecules in tissue samples?
Which observation technique can indicate the presence of radiolabeled macromolecules in tissue samples?
What is a common limitation associated with the use of scanning electron microscopy?
What is a common limitation associated with the use of scanning electron microscopy?
Study Notes
Educational Resources
- Learning aids are available as cards from Amazon and as an app for smartphones or tablets.
- McGraw-Hill medical illustrations enhance the content, making the material visually appealing and easy to understand.
- Junqueira’s Basic Histology is a widely recognized educational resource in histology, incorporating feedback from users to improve features.
Tissue Preparation Techniques
- Fixation: Essential for tissue preservation; uses stabilizing compounds to prevent degradation.
- Common fixatives include formalin (37% formaldehyde) for light microscopy and glutaraldehyde for electron microscopy.
- Tissue should be cut into small pieces or perfused with fixatives for thorough penetration.
Embedding and Sectioning
- Tissues are embedded in paraffin or plastic resins to provide firmness for sectioning.
- Paraffin is used for light microscopy, while plastic resins adapt for both light and electron microscopy.
- Thin sections are cut from the embedded tissues and placed on glass slides.
Staining Techniques
- Staining is vital for visualizing tissues under a microscope as most components are colorless.
- Different staining methods highlight various tissue features and structures, aiding in their differentiation.
Electron Microscopy
- Scanning Electron Microscopy (SEM) offers high-resolution views of cell and tissue surfaces without passing electrons through the specimen.
- The surface is dried and coated with a thin layer of metal to enhance the imaging process.
- Transmission Electron Microscopy (TEM) provides detailed images by utilizing heavy metal ions to improve contrast and resolution in tissue examination.
Autoradiography
- A method for identifying and locating radiolabeled macromolecules in tissues using photographic emulsion and radiation-sensitive silver bromide crystals.
- Allows for analysis of DNA replication in tissues by using radioactive precursors like tritium-labeled thymidine.
Observations in Histology
- Electron microscopy can reveal minute cellular structures with greater magnification and resolution than light microscopy.
- Staining aids in identifying cellular components and their locations within tissues, enhancing the study of histological samples.
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Description
Explore this valuable learning aid featuring McGraw-Hill medical illustrations. Each chapter includes art and figures designed to simplify learning and integration with related material. Available as actual cards or a smartphone app, this resource enhances the educational experience.
