Podcast
Questions and Answers
The ______ is a small cylinder attached to the upper part of the body tube that holds the ocular lens.
The ______ is a small cylinder attached to the upper part of the body tube that holds the ocular lens.
Mechanical Draw tube
The ______ connects the ocular lens to the revolving nosepiece.
The ______ connects the ocular lens to the revolving nosepiece.
Body tube
The ______ is an adjustable knob that moves either the body tube or stage upward or downward in greater increments.
The ______ is an adjustable knob that moves either the body tube or stage upward or downward in greater increments.
Coarse adjustment knob
The ______ provides firm and steady support to the entire microscope.
The ______ provides firm and steady support to the entire microscope.
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The ______ regulates how much light passes through the specimen.
The ______ regulates how much light passes through the specimen.
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The ______ is used to secure the specimen slide on the stage.
The ______ is used to secure the specimen slide on the stage.
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The ______ reflects light and directs it to the specimen slide.
The ______ reflects light and directs it to the specimen slide.
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The ______ is used to magnify the specimen under study and can come in three types.
The ______ is used to magnify the specimen under study and can come in three types.
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The ______ supports the body tube and is used to carry the microscope.
The ______ supports the body tube and is used to carry the microscope.
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The ______ is a platform where the specimen slide is placed.
The ______ is a platform where the specimen slide is placed.
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The ______ concentrates light onto the specimen and is located above the iris diaphragm.
The ______ concentrates light onto the specimen and is located above the iris diaphragm.
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The ______ is an adjustable knob that brings the specimen into sharp focus using lesser increments.
The ______ is an adjustable knob that brings the specimen into sharp focus using lesser increments.
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The ______ must be used only with the scanner or low power objective.
The ______ must be used only with the scanner or low power objective.
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The ______ is a circular part that holds the objectives and allows for their rotation.
The ______ is a circular part that holds the objectives and allows for their rotation.
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The ______ provides support above the base and is crucial for the microscope's structure.
The ______ provides support above the base and is crucial for the microscope's structure.
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The ______ has knobs to adjust its position and is vital for concentrating light.
The ______ has knobs to adjust its position and is vital for concentrating light.
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The ______ is a detachable cylinder used to view the specimen and can magnify objects up to 10x.
The ______ is a detachable cylinder used to view the specimen and can magnify objects up to 10x.
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The ______ is used to regulate light passing through the specimen and is controlled by a lever.
The ______ is used to regulate light passing through the specimen and is controlled by a lever.
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Study Notes
Mechanical Parts of a Compound Light Microscope
- Draw tube: Located at the top of the body tube, the draw tube securely holds the ocular lens.
- Body tube: The body tube connects the ocular lens to the revolving nosepiece.
- Coarse adjustment knob: This knob moves the body tube or stage significantly for initial specimen focusing. It's primarily used with the scanner or low-power objective.
- Fine adjustment knob: This knob provides precise movement of the body tube or stage for sharper focusing, mainly used with high-power and oil immersion objectives.
- Arm: Supporting the body tube, the arm is also used for carrying the microscope.
- Revolving nosepiece: This circular and revolving part holds the objective lenses and is connected to the body tube.
- Stage: The stage is a platform where the specimen slide is placed. It features a central hole for light to pass through the specimen.
- Stage clips: Stage clips secure the specimen slide in place on the stage.
- Inclination joint: Attached to the pillar, the inclination joint allows tilting the microscope.
- Pillar: The pillar provides support above the base and connects to the arm.
- Base: The base forms the foundation of the microscope, providing stable support for the whole instrument.
Optical Parts of a Compound Light Microscope
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Ocular lens (Eyepiece): The detachable ocular lens, positioned at the top of the draw tube, magnifies the specimen. Typically, it provides a 10x magnification. Some ocular lenses feature a black line as a pointer.
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Objective lenses: Multiple objective lenses with varying magnifications are used to magnify the specimen. A standard compound microscope usually has three objective lenses: low-power objective (LPO), high-power objective (HPO), and oil immersion objective (OIO). Some microscopes may include an additional scanner objective, which sometimes replaces the OIO. The order in which objective lenses are used: scanner (if present), LPO, HPO, and OIO.
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Illuminating Condenser: Located above the iris diaphragm, the illuminating condenser concentrates light onto the specimen. It might have knobs for adjusting its position.
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Iris diaphragm: The iris diaphragm regulates the intensity of light that passes through the specimen. A lever controls the diaphragm, allowing the amount of light to be adjusted.
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Mirror: Positioned beneath the stage, near the base, the mirror reflects light toward the specimen slide. The reflected image is then directed back to the observer's eyes.
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Key Points*
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The table illustrates the parts of a compound light microscope and their functions.
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The microscope consists of mechanical parts that provide framework and support, and optical parts that manage light and image formation.
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The table also highlights the order of using objective lenses and the purpose of each lens.
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The function of each part contributes to the overall process of viewing and studying specimens under the microscope.
Compound Light Microscope Parts and Functions
- Mechanical Draw Tube: A small cylinder attached to the body tube, holding the ocular lens.
- Body Tube: Connects the ocular lens to the revolving nosepiece.
- Coarse Adjustment Knob: Moves the body tube or stage in large increments for initial focusing, primarily used with the scanner or low-power objective.
- Fine Adjustment Knob: Moves the body tube or stage in smaller increments for sharp focusing, best used with high-power or oil immersion objectives.
- Arm: Supports the body tube and serves as the carrying handle for the microscope.
- Revolving Nosepiece: Circular and revolving part connected to the body tube, holding the objective lenses.
- Stage: Platform where the specimen slide is placed, featuring a central hole allowing light to pass through the specimen.
- Stage Clips: Secure the specimen slide on the stage.
- Inclination Joint: Attaches the arm to the pillar, enabling tilting of the microscope.
- Pillar: Provides support above the base, connecting to the arm.
- Base: Provides a stable foundation for the entire microscope.
- Magnifying Ocular Lens or Eyepiece: Detachable cylinder at the top of the draw tube, used for viewing the specimen, typically magnifying up to 10x. Some have a pointer for reference.
- Objective Lens: Magnifies the specimen, usually consisting of low-power (LPO), high-power (HPO), and oil immersion (OIO) lenses. Some microscopes may include a scanner objective.
- Illuminating Condenser: Concentrates light onto the specimen, located above the iris diaphragm, with adjustable knobs for positioning.
- Iris Diaphragm: Controls light passing through the specimen, using a lever to adjust the amount of light.
- Mirror: Found below the stage, near the base, it reflects light towards the specimen slide and back to the eye.
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Description
Test your knowledge on the mechanical components of a compound light microscope. This quiz covers various parts such as the draw tube, body tube, adjustment knobs, and more. Understand how each part contributes to the microscope's functionality.
