Mastering the Western Blot Technique

Which technique is used to detect specific proteins in a sample of tissue homogenate or extract?

What are the three elements used in the western blot technique?

What is the purpose of the secondary antibody in the western blot technique?

Which method is commonly used to detect biomarkers in the diagnosis of neurological and oncological illnesses?

What is the role of multidrug resistance (MDR) in cancer therapy?

Which disease can be identified through western blotting analysis of the isoform pattern of 14-3-3 proteins in cerebral fluid?

What is the main feature that sets western blotting apart from other epitope mapping techniques?

Which method was independently invented in 1979 by Jaime Renart, Jakob Reiser, and George Stark at Stanford University, and by Harry Towbin, Theophil Staehelin, and Julian Gordon at the Friedrich Miescher Institute in Basel, Switzerland?

What is the estimated percentage of protein-related publications that apply western blots?

In medical diagnostics, which test employs a western blot to detect anti-HIV antibody in a human serum sample?

Which disease can be confirmed using a western blot as the definitive test?

Western Blot Technique

The western blot technique is used to detect specific proteins in a sample of tissue homogenate or extract.
The three elements used in the western blot technique are:
- Electrotransfer of proteins to a blotting membrane
- Immunodetection with primary and secondary antibodies
- Chemiluminescent or chromogenic detection of the antigen-antibody complex

Role of Secondary Antibody

The purpose of the secondary antibody in the western blot technique is to bind to the primary antibody, amplifying the signal and allowing for detection.

Detection of Biomarkers

Western blot is a method commonly used to detect biomarkers in the diagnosis of neurological and oncological illnesses.

Multidrug Resistance (MDR) in Cancer Therapy

Multidrug resistance (MDR) plays a significant role in cancer therapy, making it challenging to treat cancer patients.

Creutzfeldt-Jakob Disease Diagnosis

Western blotting analysis of the isoform pattern of 14-3-3 proteins in cerebral fluid can identify Creutzfeldt-Jakob disease.

Unique Feature of Western Blotting

The main feature that sets western blotting apart from other epitope mapping techniques is its ability to identify specific protein isoforms.

History of Western Blot

Western blot was independently invented in 1979 by Jaime Renart, Jakob Reiser, and George Stark at Stanford University, and by Harry Towbin, Theophil Staehelin, and Julian Gordon at the Friedrich Miescher Institute in Basel, Switzerland.

Prevalence of Western Blot in Research

Approximately 10% of protein-related publications apply western blots.

Medical Diagnostics

The western blot test is employed to detect anti-HIV antibody in a human serum sample.
Western blot is used as the definitive test to confirm Lyme disease.

Test your knowledge of the western blot technique and its applications in molecular biology and immunogenetics. Learn how to detect, visualize, distinguish, and quantify specific proteins in complex protein mixtures.