Podcast
Questions and Answers
Which technique is used to detect specific proteins in a sample of tissue homogenate or extract?
Which technique is used to detect specific proteins in a sample of tissue homogenate or extract?
- Dot blot
- Northern blot
- Western blot (correct)
- Southern blot
What are the three elements used in the western blot technique?
What are the three elements used in the western blot technique?
- Separation by charge, transfer of protein to a solid support, and staining
- Separation by size, transfer of protein to a solid support, and marking target protein using a primary and secondary antibody (correct)
- Separation by charge, transfer of protein to a liquid support, and marking target protein using a primary and secondary antibody
- Separation by size, transfer of protein to a solid support, and staining
What is the purpose of the secondary antibody in the western blot technique?
What is the purpose of the secondary antibody in the western blot technique?
- To visualize the specific target protein (correct)
- To create a primary antibody
- To separate the proteins by size
- To wash off the excess primary antibody
Which method is commonly used to detect biomarkers in the diagnosis of neurological and oncological illnesses?
Which method is commonly used to detect biomarkers in the diagnosis of neurological and oncological illnesses?
What is the role of multidrug resistance (MDR) in cancer therapy?
What is the role of multidrug resistance (MDR) in cancer therapy?
Which disease can be identified through western blotting analysis of the isoform pattern of 14-3-3 proteins in cerebral fluid?
Which disease can be identified through western blotting analysis of the isoform pattern of 14-3-3 proteins in cerebral fluid?
What is the main feature that sets western blotting apart from other epitope mapping techniques?
What is the main feature that sets western blotting apart from other epitope mapping techniques?
Which method was independently invented in 1979 by Jaime Renart, Jakob Reiser, and George Stark at Stanford University, and by Harry Towbin, Theophil Staehelin, and Julian Gordon at the Friedrich Miescher Institute in Basel, Switzerland?
Which method was independently invented in 1979 by Jaime Renart, Jakob Reiser, and George Stark at Stanford University, and by Harry Towbin, Theophil Staehelin, and Julian Gordon at the Friedrich Miescher Institute in Basel, Switzerland?
What is the estimated percentage of protein-related publications that apply western blots?
What is the estimated percentage of protein-related publications that apply western blots?
In medical diagnostics, which test employs a western blot to detect anti-HIV antibody in a human serum sample?
In medical diagnostics, which test employs a western blot to detect anti-HIV antibody in a human serum sample?
Which disease can be confirmed using a western blot as the definitive test?
Which disease can be confirmed using a western blot as the definitive test?
Study Notes
Western Blot Technique
- The western blot technique is used to detect specific proteins in a sample of tissue homogenate or extract.
- The three elements used in the western blot technique are:
- Electrotransfer of proteins to a blotting membrane
- Immunodetection with primary and secondary antibodies
- Chemiluminescent or chromogenic detection of the antigen-antibody complex
Role of Secondary Antibody
- The purpose of the secondary antibody in the western blot technique is to bind to the primary antibody, amplifying the signal and allowing for detection.
Detection of Biomarkers
- Western blot is a method commonly used to detect biomarkers in the diagnosis of neurological and oncological illnesses.
Multidrug Resistance (MDR) in Cancer Therapy
- Multidrug resistance (MDR) plays a significant role in cancer therapy, making it challenging to treat cancer patients.
Creutzfeldt-Jakob Disease Diagnosis
- Western blotting analysis of the isoform pattern of 14-3-3 proteins in cerebral fluid can identify Creutzfeldt-Jakob disease.
Unique Feature of Western Blotting
- The main feature that sets western blotting apart from other epitope mapping techniques is its ability to identify specific protein isoforms.
History of Western Blot
- Western blot was independently invented in 1979 by Jaime Renart, Jakob Reiser, and George Stark at Stanford University, and by Harry Towbin, Theophil Staehelin, and Julian Gordon at the Friedrich Miescher Institute in Basel, Switzerland.
Prevalence of Western Blot in Research
- Approximately 10% of protein-related publications apply western blots.
Medical Diagnostics
- The western blot test is employed to detect anti-HIV antibody in a human serum sample.
- Western blot is used as the definitive test to confirm Lyme disease.
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Description
Test your knowledge of the western blot technique and its applications in molecular biology and immunogenetics. Learn how to detect, visualize, distinguish, and quantify specific proteins in complex protein mixtures.
