Mastering Molecular Biotechnology
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Questions and Answers

Which technique is also known as Sucrose density gradient?

  • Methods of Making Probes
  • Electrophoresis
  • Centrifugation (correct)
  • Blotting Techniques

What is the purpose of Equilibrium Density Gradient technique?

  • To separate DNA of different MW
  • To separate mixture of DNA, RNA & proteins (correct)
  • To separate molecules based on differences in density
  • To separate proteins based on their charge

What is the highest density component in the Equilibrium Density Gradient technique?

  • CsCl
  • DNA
  • RNA (correct)
  • Proteins

What stops molecules from moving in the Equilibrium Density Gradient technique?

<p>Density of the medium (D)</p> Signup and view all the answers

Which component is less negatively charged than DNA/RNA in the Equilibrium Density Gradient technique?

<p>Proteins (B)</p> Signup and view all the answers

Which technique is also known as Sucrose density gradient?

<p>Centrifugation (A)</p> Signup and view all the answers

Which technique separates molecules based on differences in density?

<p>Centrifugation (B)</p> Signup and view all the answers

Which molecule has the highest density in the equilibrium density gradient technique?

<p>RNA (B)</p> Signup and view all the answers

What happens when the density of molecules is equal to the density of the medium in the equilibrium density gradient technique?

<p>Molecules stop moving (B)</p> Signup and view all the answers

Which technique is used to separate DNA of different molecular weight?

<p>Centrifugation (B)</p> Signup and view all the answers

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