Questions and Answers
Which of the following is a characteristic of Corynebacterium?
Gram-positive
Which species of Corynebacterium causes diphtheria?
C.diphtheriae
What is the major infection caused by C.diphtheriae?
Respiratory diphtheria
How is diphtheria diagnosed in the laboratory?
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What type of stain is used to visualize Corynebacterium in the laboratory?
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Which selective media is used to differentiate commensal species from C. diphtheriae?
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What is the purpose of using tellurite salt in Hoyle's Tellurite blood agar?
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Which biochemical test is used for the identification of C. ulcerans?
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What is the mainstay of treatment for diphtheria?
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Which antibiotics are sensitive to C. diphtheriae?
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Study Notes
Characteristics of Corynebacterium
- Corynebacterium species possess characteristic club-shaped or filamentous morphology
Diphtheria and C. diphtheriae
- C. diphtheriae causes diphtheria
- Diphtheria is the major infection caused by C. diphtheriae
Laboratory Diagnosis of Diphtheria
- Diphtheria is diagnosed in the laboratory through laboratory tests
- Palkovac or Loeffler's medium is used to visualize Corynebacterium in the laboratory
- Tellurite agar or Hoyle's Tellurite blood agar is used to differentiate commensal species from C. diphtheriae
- Tellurite salt in Hoyle's Tellurite blood agar inhibits growth of other bacteria, allowing selective growth of C. diphtheriae
Identification of C. ulcerans
- C. ulcerans is identified through a biochemical test, such as the Elek test
Treatment of Diphtheria
- The mainstay of treatment for diphtheria is antitoxin administration
- Antibiotics such as erythromycin and penicillin are sensitive to C. diphtheriae
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