Microbial Regulatory System (Lec #9)

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24 Questions

Which enzyme is responsible for DNA replication?

DNA polymerase

Which molecule is the template for RNA synthesis during transcription?

DNA

What is the major mode of regulation for gene expression in bacteria?

Transcription

What is the function of mRNA during protein synthesis?

Template for protein synthesis

What is the role of tRNA during protein synthesis?

Brings amino acids to the ribosome

What is the function of RNA polymerase?

Catalyzes transcription of DNA into RNA

What is an operon in bacteria?

A group of genes transcribed into a single mRNA

Which type of control mechanism prevents the synthesis of an enzyme in response to a signal?

Repression

What is the effector molecule called that binds to the repressor and alters its conformation in the mechanism of repression?

Co-repressor

Which type of control mechanism involves the production of an enzyme in response to a signal?

Induction

What is the effector molecule that binds to the repressor and alters its conformation in the mechanism of induction?

Inducer

What is the regulatory protein that binds to an activator binding site near the promoter and helps RNA polymerase bind to the DNA in positive control?

Activator

What is the term for multiple operons controlled by the same regulatory protein?

Regulon

What is the mechanism of an activator that involves bending DNA to allow for RNA polymerase binding?

Looping

Which sigma factor triggers sporulation in Bacillus subtilis?

Spo0A

What controls endospore formation?

Sigma Factors

In Quorum Sensing what was the first autoinducer identified?

Acyl Homoserine lactones (AHLs)

Which of the following is true about catabolite repression in prokaryotes?

Catabolite repression ensures that the 'best' carbon and energy source is used first

Which protein acts as an activator in catabolite repression?

Cyclic AMP receptor protein (CRP)

What happens to cyclic AMP levels when glucose levels are high?

Cyclic AMP levels decrease

What is the role of the lac repressor in the lac operon?

It binds to the operator and prevents transcription of the lac genes

What is the function of the sensor kinase in a two-component regulatory system?

It detects a signal from the environment and phosphorylates itself

What is the purpose of quorum sensing in prokaryotes?

To monitor population size and control gene expression based on cell density

What is the role of sigma factors in gene expression control?

They interact with consensus sequences at the promoter

Test your knowledge on global control and the lac operon with this quiz! Explore topics such as catabolite repression, activator proteins, and the role of RNA polymerase in regulating catabolic operons. Challenge yourself and see how well you understand the intricacies of allosteric regulation and the utilization of different carbon and energy sources.

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