Podcast
Questions and Answers
Which microscopy technique is used to visualize cells and their molecules?
Which microscopy technique is used to visualize cells and their molecules?
- Live imaging
- Fluorescent microscopy (correct)
- Confocal laser scanning microscopy
- Fixed cell samples
When would you prefer to use confocal laser scanning microscopy over regular fluorescent microscopy?
When would you prefer to use confocal laser scanning microscopy over regular fluorescent microscopy?
- When you need high resolution imaging
- When you need to visualize multiple focal planes (correct)
- When you need to visualize fixed cell samples
- When you need to visualize live cells
What are the criteria to consider when deciding between live imaging and fixed cell samples for an experiment?
What are the criteria to consider when deciding between live imaging and fixed cell samples for an experiment?
- Microscopy techniques available
- Nature of the experiment (correct)
- Availability of equipment
- Resolution required
How can imaging be used to test if two proteins form a complex in the cell?
How can imaging be used to test if two proteins form a complex in the cell?
Which imaging technique would you use to test if two compartments of the cell are connected?
Which imaging technique would you use to test if two compartments of the cell are connected?
Which vesicles are involved in anterograde transport?
Which vesicles are involved in anterograde transport?
How do proteins reach the Golgi from the ER in animals?
How do proteins reach the Golgi from the ER in animals?
How do proteins migrate through the Golgi?
How do proteins migrate through the Golgi?
How are ER proteins retrieved from the Golgi?
How are ER proteins retrieved from the Golgi?
What is the fate of the Golgi during the cell cycle in animals?
What is the fate of the Golgi during the cell cycle in animals?