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Mastering Cell Visualization with Fluorescent and Confocal Microscopy

10 Questions

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Mastering Cell Visualization with Fluorescent and Confocal Microscopy

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Questions and Answers

Which microscopy technique is used to visualize cells and their molecules?

Live imaging

Fluorescent microscopy (correct)

Confocal laser scanning microscopy

Fixed cell samples

When would you prefer to use confocal laser scanning microscopy over regular fluorescent microscopy?

When you need high resolution imaging

When you need to visualize multiple focal planes (correct)

When you need to visualize fixed cell samples

When you need to visualize live cells

What are the criteria to consider when deciding between live imaging and fixed cell samples for an experiment?

Microscopy techniques available

Nature of the experiment (correct)

Availability of equipment

Resolution required

How can imaging be used to test if two proteins form a complex in the cell?

By visualizing their interaction using a specific dye Signup and view all the answers

Which imaging technique would you use to test if two compartments of the cell are connected?

Total internal reflection fluorescence microscopy Signup and view all the answers

Which vesicles are involved in anterograde transport?

COPII vesicles Signup and view all the answers

How do proteins reach the Golgi from the ER in animals?

Through COPII vesicles Signup and view all the answers

How do proteins migrate through the Golgi?

By diffusion Signup and view all the answers

How are ER proteins retrieved from the Golgi?

Through COPI vesicles Signup and view all the answers

What is the fate of the Golgi during the cell cycle in animals?

It disintegrates Signup and view all the answers