Mastering Cell Visualization with Fluorescent and Confocal Microscopy
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Questions and Answers

Which microscopy technique is used to visualize cells and their molecules?

  • Live imaging
  • Fluorescent microscopy (correct)
  • Confocal laser scanning microscopy
  • Fixed cell samples

When would you prefer to use confocal laser scanning microscopy over regular fluorescent microscopy?

  • When you need high resolution imaging
  • When you need to visualize multiple focal planes (correct)
  • When you need to visualize fixed cell samples
  • When you need to visualize live cells

What are the criteria to consider when deciding between live imaging and fixed cell samples for an experiment?

  • Microscopy techniques available
  • Nature of the experiment (correct)
  • Availability of equipment
  • Resolution required

How can imaging be used to test if two proteins form a complex in the cell?

<p>By visualizing their interaction using a specific dye (D)</p> Signup and view all the answers

Which imaging technique would you use to test if two compartments of the cell are connected?

<p>Total internal reflection fluorescence microscopy (C)</p> Signup and view all the answers

Which vesicles are involved in anterograde transport?

<p>COPII vesicles (D)</p> Signup and view all the answers

How do proteins reach the Golgi from the ER in animals?

<p>Through COPII vesicles (A)</p> Signup and view all the answers

How do proteins migrate through the Golgi?

<p>By diffusion (C)</p> Signup and view all the answers

How are ER proteins retrieved from the Golgi?

<p>Through COPI vesicles (C)</p> Signup and view all the answers

What is the fate of the Golgi during the cell cycle in animals?

<p>It disintegrates (B)</p> Signup and view all the answers

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