3 Questions
Which enzyme removes the variant unedited 5' flap in the CRISPR-Cas9 system?
FEN1
What is the role of the 3' flap in the CRISPR-Cas9 system?
It is used as a template for new DNA synthesis
Where does the nick occur in the CRISPR-Cas9 system?
3 bp upstream of the PAM site
Test your knowledge of the Cas9-pegRNA complex and its role in gene editing. Learn about the binding process, nick formation, and the interaction between the 3' flap and the primer binding site.
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