Mastering Cas9-pegRNA Complex
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Questions and Answers

Which enzyme removes the variant unedited 5' flap in the CRISPR-Cas9 system?

  • PAM site
  • RT polymerase
  • Cas9
  • FEN1 (correct)

    • What is the role of the 3' flap in the CRISPR-Cas9 system?

  • It is used as a template for new DNA synthesis (correct)
  • It binds to the desired target region
  • It creates a nick upstream of the PAM site
  • It forms a sequence-specific interaction with the 14-16 nt 'primer binding site'

    • Where does the nick occur in the CRISPR-Cas9 system?

  • On the opposite strand of the PAM
  • 3 bp upstream of the PAM site (correct)
  • At the variant unedited 5' flap
  • 3 bp downstream of the PAM site

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