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Questions and Answers
Which enzyme removes the variant unedited 5' flap in the CRISPR-Cas9 system?
Which enzyme removes the variant unedited 5' flap in the CRISPR-Cas9 system?
- PAM site
- RT polymerase
- Cas9
- FEN1 (correct)
What is the role of the 3' flap in the CRISPR-Cas9 system?
What is the role of the 3' flap in the CRISPR-Cas9 system?
- It is used as a template for new DNA synthesis (correct)
- It binds to the desired target region
- It creates a nick upstream of the PAM site
- It forms a sequence-specific interaction with the 14-16 nt 'primer binding site'
Where does the nick occur in the CRISPR-Cas9 system?
Where does the nick occur in the CRISPR-Cas9 system?
- On the opposite strand of the PAM
- 3 bp upstream of the PAM site (correct)
- At the variant unedited 5' flap
- 3 bp downstream of the PAM site
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