3 Questions
What is the purpose of adding Molisch’s reagent to the carbohydrate solution?
To test for the presence of carbohydrates
What is the role of concentrated H2SO4 in the Molisch’s test procedure?
To form a layer at the bottom of the test tube
What is observed in the Molisch’s test for carbohydrates?
Formation of a color between the two layers
Study Notes
Molisch's Test for Carbohydrates
- Molisch's reagent is added to the carbohydrate solution to detect the presence of carbohydrates, specifically, to identify the presence of glycols or polyhydroxy compounds.
- Concentrated H2SO4 is used to break down the carbohydrate molecules into simpler glycols, which then react with the α-naphthol in Molisch's reagent to produce a purple-colored complex.
- A purple ring or precipitate is observed at the junction of the two liquids in the Molisch's test, indicating the presence of carbohydrates.
Test your knowledge of carbohydrate analysis with this quiz on Molisch’s reagent. Learn about the procedure for using Molisch’s reagent to detect the presence of carbohydrates and the color formation in the test tube.
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