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Questions and Answers
What is the purpose of Molisch's Test?
Molisch's Test is a general test for carbohydrates, detecting the presence of sugars through the formation of furfural and its derivatives when acted upon by strong mineral acids.
What causes the purple color observed in Molisch's Test?
The purple color observed in Molisch's Test is due to the condensation of furfural and its derivatives with two molecules of phenol, typically α-naphthol.
What can concentrated sugar solutions give with H2SO4, and what is the cause of this observation?
Concentrated sugar solutions may give dark brown to red solutions with H2SO4 due to the charring action of the acid and the formation of resinous products.
What is the purpose of performing colour tests for carbohydrates?
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What reagent is used in Molisch's Test and what is its function?
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What is the principle behind Benedict's Test for reducing sugars?
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How can non-reducing sugars be distinguished from reducing sugars in the carbohydrate tests?
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What is the significance of Lugol's Iodine in the carbohydrate tests?
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