Molisch’s Test Quiz
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Questions and Answers

What is the purpose of Molisch's Test?

Molisch's Test is a general test for carbohydrates, detecting the presence of sugars through the formation of furfural and its derivatives when acted upon by strong mineral acids.

What causes the purple color observed in Molisch's Test?

The purple color observed in Molisch's Test is due to the condensation of furfural and its derivatives with two molecules of phenol, typically α-naphthol.

What can concentrated sugar solutions give with H2SO4, and what is the cause of this observation?

Concentrated sugar solutions may give dark brown to red solutions with H2SO4 due to the charring action of the acid and the formation of resinous products.

What is the purpose of performing colour tests for carbohydrates?

<p>The purpose is to identify the presence of different types of carbohydrates based on their reactions with specific reagents.</p> Signup and view all the answers

What reagent is used in Molisch's Test and what is its function?

<p>Molisch reagent is used, and it helps in detecting the presence of carbohydrates by forming a purple ring at the junction of the reagent and the test solution.</p> Signup and view all the answers

What is the principle behind Benedict's Test for reducing sugars?

<p>Benedict's Test is based on the reduction of cupric ions by reducing sugars, leading to the formation of a red precipitate.</p> Signup and view all the answers

How can non-reducing sugars be distinguished from reducing sugars in the carbohydrate tests?

<p>Non-reducing sugars, such as sucrose, can be distinguished by performing additional tests like Barfoed's Test, which specifically detects reducing sugars.</p> Signup and view all the answers

What is the significance of Lugol's Iodine in the carbohydrate tests?

<p>Lugol's Iodine is used to test for the presence of starch, as it forms a blue-black color complex with starch molecules.</p> Signup and view all the answers

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