Podcast
Questions and Answers
During bidirectional replication, how many growing points start at the same site and move in opposite directions?
During bidirectional replication, how many growing points start at the same site and move in opposite directions?
- Two (correct)
- One
- Four
- Three
What did John Cairns' autoradiography studies show about E. coli DNA?
What did John Cairns' autoradiography studies show about E. coli DNA?
- It is circular and cannot form theta structures during replication
- It is linear and can form theta structures during replication
- It is linear and cannot form theta structures during replication
- It is circular and can form theta structures during replication (correct)
Which type of replication involves a single growing point moving around the circular DNA until replication is complete?
Which type of replication involves a single growing point moving around the circular DNA until replication is complete?
- Semidiscontinuous replication
- Unidirectional replication (correct)
- Discontinuous replication
- Bidirectional replication
What is the overall direction of the DNA strand that grows discontinuously during replication?
What is the overall direction of the DNA strand that grows discontinuously during replication?
Who proposed the discontinuous and semidiscontinuous models of replication for the strand growing in an overall 3′→5′ direction?
Who proposed the discontinuous and semidiscontinuous models of replication for the strand growing in an overall 3′→5′ direction?
What is the length of the adjacent short fragments formed during eukaryotic DNA replication?
What is the length of the adjacent short fragments formed during eukaryotic DNA replication?
What type of replication has been observed in all organisms and DNA viruses studied to date?
What type of replication has been observed in all organisms and DNA viruses studied to date?
What type of replication leads to a short single-stranded region at the replication fork on the template strand for discontinuous DNA replication?
What type of replication leads to a short single-stranded region at the replication fork on the template strand for discontinuous DNA replication?
What was the mistake made about E. coli DNA molecules at the time of Meselson and Stahl's experiment?
What was the mistake made about E. coli DNA molecules at the time of Meselson and Stahl's experiment?
What can radioisotope labeling experiments distinguish between during DNA replication?
What can radioisotope labeling experiments distinguish between during DNA replication?
During bidirectional replication, how many growing points start at the same site and move in opposite directions until they meet at the opposite side of the circle?
During bidirectional replication, how many growing points start at the same site and move in opposite directions until they meet at the opposite side of the circle?
What did John Cairns' autoradiography studies show about E. coli DNA?
What did John Cairns' autoradiography studies show about E. coli DNA?
What distinguishes bidirectional and unidirectional DNA replication in radioisotope labeling experiments?
What distinguishes bidirectional and unidirectional DNA replication in radioisotope labeling experiments?
Which direction does the DNA strand grow in during semidiscontinuous replication?
Which direction does the DNA strand grow in during semidiscontinuous replication?
Who proposed the discontinuous and semidiscontinuous models of replication for the strand growing in an overall 3′→5′ direction?
Who proposed the discontinuous and semidiscontinuous models of replication for the strand growing in an overall 3′→5′ direction?
What is the length of the adjacent short fragments formed during eukaryotic DNA replication?
What is the length of the adjacent short fragments formed during eukaryotic DNA replication?
What is the result of semidiscontinuous replication at the replication fork on the template strand for discontinuous DNA replication?
What is the result of semidiscontinuous replication at the replication fork on the template strand for discontinuous DNA replication?
What is the overall direction of DNA synthesis during semidiscontinuous replication?
What is the overall direction of DNA synthesis during semidiscontinuous replication?
What is the difference between bidirectional and unidirectional DNA replication?
What is the difference between bidirectional and unidirectional DNA replication?
What did Meselson and Stahl's experiment mistakenly assume about E. coli DNA molecules?
What did Meselson and Stahl's experiment mistakenly assume about E. coli DNA molecules?
Flashcards are hidden until you start studying
Study Notes
Bidirectional and Semidiscontinuous DNA Replication
- Bacterial and eukaryotic DNA replication is bidirectional.
- At the time of Meselson and Stahl's experiment, E. coli DNA molecules were mistakenly believed to be linear duplexes.
- John Cairns' autoradiography studies in 1963 showed E. coli DNA is circular and can form theta structures during replication.
- Bidirectional replication involves two growing points starting at the same site and moving in opposite directions until they meet at the opposite side of the circle.
- Unidirectional replication involves a single growing point moving around the circular DNA until replication is complete.
- Radioisotope labeling experiments can distinguish between bidirectional and unidirectional replication.
- DNA replication in bacteria and eukaryotes is bidirectional.
- The DNA strand that grows in an overall 3′→5′ direction is synthesized discontinuously.
- Reiji Okazaki proposed the discontinuous and semidiscontinuous models of replication for the strand growing in an overall 3′→5′ direction.
- Semidiscontinuous replication has been observed in all organisms and DNA viruses studied to date.
- The adjacent short fragments formed during eukaryotic DNA replication are about 100 to 200 nucleotides long.
- Semidiscontinuous replication leads to a short single-stranded region at the replication fork on the template strand for discontinuous DNA replication.
Studying That Suits You
Use AI to generate personalized quizzes and flashcards to suit your learning preferences.
