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Lowry's Method: Measurement of Total Protein

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Lowry's Method: Measurement of Total Protein

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Questions and Answers

What is the purpose of adding Folin's reagent in Lowry's method?

To catalyze the reaction of alkaline copper with protein

To reduce the phosphomolybdate (correct)

To inhibit the reaction of tyrosine and tryptophan

To enhance the Biuret reaction

What is the wavelength at which the blue complex absorbs maximum light?

740nm (correct)

620nm

560nm

840nm

What is the ratio of the intensity of the light entering a sample to the intensity of the light exiting a sample called?

Reflectance

Refractance

Transmittance (correct)

Absorbance

Which two amino acids are responsible for the reduction of phosphomolybdate in Lowry's method?

Tyrosine and tryptophan Signup and view all the answers

What is the purpose of the alkaline copper solution in Lowry's method?

To chelate with the peptide bonds Signup and view all the answers

What is the principle of the spectrophotometer?

Measures the intensity of the light entering and exiting a sample Signup and view all the answers

What is the primary reason for choosing a specific wavelength in quantitative analysis?

To ensure that the substance absorbs the wavelength appreciably Signup and view all the answers

What is the optimal transmission range for minimizing error in reading absorbance?

36.8% T Signup and view all the answers

What is the relationship between the concentration of the absorbing medium and the intensity of the monochromatic light according to Beer's Law?

The intensity of light decreases exponentially with concentration Signup and view all the answers

What is the product of the molar extinction coefficient, concentration, and path length in the combined Beer-Lambert Law?

kcl Signup and view all the answers

What is the quantity represented by the symbol 'T' in the context of quantitative analysis?

Transmittance Signup and view all the answers

What is the condition represented by an absorbance value of 0?

No photons are absorbed by the analyte Signup and view all the answers