12 Questions
What is the purpose of adding Folin's reagent in Lowry's method?
To reduce the phosphomolybdate
What is the wavelength at which the blue complex absorbs maximum light?
740nm
What is the ratio of the intensity of the light entering a sample to the intensity of the light exiting a sample called?
Transmittance
Which two amino acids are responsible for the reduction of phosphomolybdate in Lowry's method?
Tyrosine and tryptophan
What is the purpose of the alkaline copper solution in Lowry's method?
To chelate with the peptide bonds
What is the principle of the spectrophotometer?
Measures the intensity of the light entering and exiting a sample
What is the primary reason for choosing a specific wavelength in quantitative analysis?
To ensure that the substance absorbs the wavelength appreciably
What is the optimal transmission range for minimizing error in reading absorbance?
36.8% T
What is the relationship between the concentration of the absorbing medium and the intensity of the monochromatic light according to Beer's Law?
The intensity of light decreases exponentially with concentration
What is the product of the molar extinction coefficient, concentration, and path length in the combined Beer-Lambert Law?
kcl
What is the quantity represented by the symbol 'T' in the context of quantitative analysis?
Transmittance
What is the condition represented by an absorbance value of 0?
No photons are absorbed by the analyte
This quiz covers the theory of Lowry's method, a technique used to measure total protein in a given sample. It involves the Biuret reaction, alkaline copper solution, and Folin's reagent to form a characteristic blue colour. Test your knowledge of this biochemical assay!
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