Podcast
Questions and Answers
What is the purpose of adding Folin's reagent in Lowry's method?
What is the purpose of adding Folin's reagent in Lowry's method?
- To catalyze the reaction of alkaline copper with protein
- To reduce the phosphomolybdate (correct)
- To inhibit the reaction of tyrosine and tryptophan
- To enhance the Biuret reaction
What is the wavelength at which the blue complex absorbs maximum light?
What is the wavelength at which the blue complex absorbs maximum light?
- 740nm (correct)
- 620nm
- 560nm
- 840nm
What is the ratio of the intensity of the light entering a sample to the intensity of the light exiting a sample called?
What is the ratio of the intensity of the light entering a sample to the intensity of the light exiting a sample called?
- Reflectance
- Refractance
- Transmittance (correct)
- Absorbance
Which two amino acids are responsible for the reduction of phosphomolybdate in Lowry's method?
Which two amino acids are responsible for the reduction of phosphomolybdate in Lowry's method?
What is the purpose of the alkaline copper solution in Lowry's method?
What is the purpose of the alkaline copper solution in Lowry's method?
What is the principle of the spectrophotometer?
What is the principle of the spectrophotometer?
What is the primary reason for choosing a specific wavelength in quantitative analysis?
What is the primary reason for choosing a specific wavelength in quantitative analysis?
What is the optimal transmission range for minimizing error in reading absorbance?
What is the optimal transmission range for minimizing error in reading absorbance?
What is the relationship between the concentration of the absorbing medium and the intensity of the monochromatic light according to Beer's Law?
What is the relationship between the concentration of the absorbing medium and the intensity of the monochromatic light according to Beer's Law?
What is the product of the molar extinction coefficient, concentration, and path length in the combined Beer-Lambert Law?
What is the product of the molar extinction coefficient, concentration, and path length in the combined Beer-Lambert Law?
What is the quantity represented by the symbol 'T' in the context of quantitative analysis?
What is the quantity represented by the symbol 'T' in the context of quantitative analysis?
What is the condition represented by an absorbance value of 0?
What is the condition represented by an absorbance value of 0?
Flashcards are hidden until you start studying
