Lipids and Lipoproteins in Clinical Chemistry
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Questions and Answers

What is the recommended fasting period before venipuncture for measuring Total Cholesterol (TC) and High-Density Lipoproteins (HDL-C) levels?

  • 8 hours
  • 6 hours
  • 12 hours (correct)
  • 24 hours
  • Which lipoproteins can be measured in non-fasting individuals?

  • Triglycerides (TAG)
  • Total Cholesterol (TC)
  • Low-Density Lipoproteins (LDL-C)
  • High-Density Lipoproteins (HDL-C) (correct)
  • What percentage decrease in Triglycerides (TAG) is observed from upright to supine position?

  • 10%
  • 50% (correct)
  • 75%
  • 25%
  • What causes hemoconcentration, as mentioned in the text?

    <p>Squeezing or probing a vein excessively (D)</p> Signup and view all the answers

    According to the National Cholesterol Education Program (NCEP), how long should patients be seated prior to venipuncture?

    <p>5 minutes (C)</p> Signup and view all the answers

    What is the recommended fasting period before venipuncture for measuring Triglycerides (TAG) and Low-Density Lipoproteins (LDL-C) levels?

    <p>At least 12 hours (C)</p> Signup and view all the answers

    What effect does estrogen have on lipoproteins?

    <p>Increases HDL and decreases LDL (A)</p> Signup and view all the answers

    Which lipoproteins can be measured in non-fasting individuals?

    <p>Total Cholesterol (TC) and High-Density Lipoproteins (HDL-C) (A)</p> Signup and view all the answers

    What effect do postural changes have on Triglycerides (TAG)?

    <p>Decrease by almost 50% from upright to supine position (A)</p> Signup and view all the answers

    What should not be applied for longer than a minute or two, if possible, according to the text?

    <p>Tourniquets (B)</p> Signup and view all the answers

    Flashcards

    Fasting for TC and HDL-C

    The recommended fasting period before a blood test to measure total cholesterol (TC) and high-density lipoprotein (HDL-C) levels is 12 hours.

    HDL-C measurement

    High-density lipoprotein (HDL-C) levels can be measured even without fasting before a blood test.

    Triglycerides and Posture

    Triglyceride levels can be measured without fasting. However, it's important to note that the triglyceride levels decrease significantly by almost 50% when a person goes from an upright to supine position.

    Hemoconcentration

    Squeezing or excessive probing of a vein during blood collection can lead to hemoconcentration. This is because it forces fluid out of the blood vessels, concentrating the blood components like red blood cells.

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    NCEP Seated Time

    The National Cholesterol Education Program (NCEP) recommends that patients sit for at least 5 minutes before a blood test to ensure accurate lipid measurements.

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    Fasting for TAG and LDL-C

    The recommended fasting period before a blood test to measure triglycerides (TAG) and low-density lipoprotein (LDL-C) levels is at least 12 hours.

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    Estrogen and Lipoproteins

    Estrogen, a female sex hormone, can have significant effects on lipoprotein levels. It increases the levels of high-density lipoprotein (HDL) and decreases the levels of low-density lipoprotein (LDL).

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    Non-Fasting Lipids

    Total cholesterol (TC) and high-density lipoprotein (HDL-C) levels can be measured without fasting before a blood test.

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    Postural Effects on TAG

    Postural changes, specifically going from an upright to a supine position, can decrease triglyceride (TAG) levels by almost 50%.

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    Tourniquet Duration

    Tourniquets should not be applied for longer than a minute or two, if possible, to avoid errors in blood collection and potential discomfort for the patient.

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    Study Notes

    Lipids and Lipoproteins

    • Lipids and lipoproteins are important indicators of coronary heart disease (CHD) risk.
    • The lipid profile recommended for initial screening in adults (age 20 or older) includes:
      • Total Cholesterol (TC)
      • Low-Density Lipoprotein (LDL)
      • High-Density Lipoprotein (HDL)
      • Triglycerides (TAG)
    • Testing should be repeated at least once every 5 years.

    Major Forms of Lipids

    • Triglycerides: main storage form of lipid in humans.
    • Cholesterol: part of the cell membrane; parent chain for cholesterol-based hormones (e.g. aldosterone, cortisol, sex hormones).
    • Phospholipids: most abundant lipid in humans.
    • Fatty Acids: building blocks of lipids.
    • Fat Soluble Vitamins: A, D, E, and K.

    Blood Sampling and Storage

    • Individuals should be on their usual diet for 2 weeks and be neither gaining nor losing weight prior to cholesterol measurement.
    • Effects of dietary modification take several weeks to become apparent.
    • Certain drugs, such as oral contraceptives, postmenopausal estrogens, and some antihypertensive drugs, significantly alter lipid levels.

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    Description

    Test your knowledge about lipids and lipoproteins as important indicators of coronary heart disease (CHD) risk. Understand the lipid profile recommended for initial screening in adults and the major forms of lipids and lipoproteins.

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