Podcast
Questions and Answers
What is one way to assess ligand specificity?
What is one way to assess ligand specificity?
Competitive-binding assay
What is the property of biological receptors binding tightly to a single natural ligand called?
What is the property of biological receptors binding tightly to a single natural ligand called?
Specificity
How can receptor specificity be confirmed in a competitive-binding assay?
How can receptor specificity be confirmed in a competitive-binding assay?
The amount of ligand bound is not affected by the presence of other ligands
What is the reason behind proteins tending to stick to glass?
What is the reason behind proteins tending to stick to glass?
What is the reason behind proteins having some affinity for cell surfaces?
What is the reason behind proteins having some affinity for cell surfaces?
How do molecules interact with each other?
How do molecules interact with each other?
What special statistical effects need to be considered when interpreting the nonlinear Scatchard plots that arise from lattice binding?
What special statistical effects need to be considered when interpreting the nonlinear Scatchard plots that arise from lattice binding?
How can Scatchard plots alone not distinguish between heterogeneous binding sites and cooperativity?
How can Scatchard plots alone not distinguish between heterogeneous binding sites and cooperativity?
How can cooperativity and receptor heterogeneity be distinguished?
How can cooperativity and receptor heterogeneity be distinguished?
How is the value of koff measured?
How is the value of koff measured?
In practice, how is the decrease in the concentration of bound ligand usually measured?
In practice, how is the decrease in the concentration of bound ligand usually measured?
What might limit the access of additional molecules of ligand to free sites on the lattice?
What might limit the access of additional molecules of ligand to free sites on the lattice?
What are the types of electrostatic forces that contribute to the innate affinity between molecules?
What are the types of electrostatic forces that contribute to the innate affinity between molecules?
Why are noncovalent interactions important in biological processes?
Why are noncovalent interactions important in biological processes?
What do receptors and ligands exchange interactions with before interacting with each other?
What do receptors and ligands exchange interactions with before interacting with each other?
What are biological chemists trying to understand regarding receptor-ligand interactions?
What are biological chemists trying to understand regarding receptor-ligand interactions?
What is the goal of medicinal chemists in relation to receptor-ligand interactions?
What is the goal of medicinal chemists in relation to receptor-ligand interactions?
Why are organic chemists trying to develop synthetic systems that mimic biological interactions?
Why are organic chemists trying to develop synthetic systems that mimic biological interactions?
