Ligand Binding Heterogeneity and Cooperativity in Receptor Systems

Competitive-binding assay

Specificity

The amount of ligand bound is not affected by the presence of other ligands

<p>Polar surfaces interacting with each other</p> Signup and view all the answers

<p>Cell surfaces being quite polar</p> Signup and view all the answers

<p>Noncovalently</p> Signup and view all the answers

<p>Special statistical effects need to be considered when interpreting the nonlinear Scatchard plots that arise from lattice binding.</p> Signup and view all the answers

<p>Scatchard plots alone cannot distinguish between heterogeneous binding sites and cooperativity because they both result in nonlinear plots.</p> Signup and view all the answers

<p>Cooperativity and receptor heterogeneity can be distinguished by measuring the effect of bound ligand on the value of the dissociation rate constant, koff.</p> Signup and view all the answers

<p>The value of koff can be measured by allowing ligand and receptor to reach equilibrium, removing free ligand, and then measuring the dissociation of ligand over time.</p> Signup and view all the answers

<p>In practice, the decrease in the concentration of bound ligand is usually measured.</p> Signup and view all the answers

<p>Certain arrangements of large ligands on lattice-like receptors may limit the access of additional molecules of ligand to free sites on the lattice.</p> Signup and view all the answers

<p>Coulombic attractions, hydrogen bonds, and dispersion forces</p> Signup and view all the answers

<p>They are important for catalysis of chemical reactions, neutralization of foreign toxins, and stimulation of cellular activities.</p> Signup and view all the answers

<p>Solvent and solute molecules</p> Signup and view all the answers

<p>The molecular details</p> Signup and view all the answers

<p>To develop useful pharmaceutics</p> Signup and view all the answers

<p>To mimic biological interactions</p> Signup and view all the answers