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Laboratory Techniques for Handling Liquid Volumes

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Laboratory Techniques for Handling Liquid Volumes

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Questions and Answers

What is the purpose of the direct plate exposure method?

To flood the surface of a solid medium with a liquid inoculum
To spread a liquid inoculum over the surface of a solid medium
To sample microorganisms from aerosol or the surrounding air (correct)
To distribute the inoculum into the agar medium

What is the primary difference between the spread plate and flood plate methods?

The spread plate produces a lawn plate, while the flood plate does not
The spread plate uses a measured volume of inoculum, while the flood plate uses an unmeasured amount
The spread plate inoculates the surface, while the flood plate inoculates the subsurface
The spread plate uses a sterile bent glass rod to spread the inoculum, while the flood plate floods the surface with the inoculum (correct)

What is the purpose of the pour plate method?

To inoculate the surface of a solid medium
To inoculate the deeper, microaerobic or anaerobic portions of a solid medium
To sample microorganisms from aerosol or the surrounding air
To evenly distribute the inoculum throughout the agar medium (correct)

When would you use a sterile swab to inoculate an agar plate?

To sample microorganisms from a dry surface (C) Signup and view all the answers

What is the purpose of the 'stabbing' inoculation method for solid tube media?

To inoculate the deeper, microaerobic or anaerobic portions of a butt (A) Signup and view all the answers

What is the purpose of plugging pipettes with cotton at the region near the aspirator before sterilization?

To filter out microbial contaminants in the air coming from the aspirator during use (A) Signup and view all the answers

Which of the following is the correct way to handle used pipettes?

Immediately place them in a vessel containing disinfectant until they are safe to handle, then wash, sterilize and re-use them (C) Signup and view all the answers

What is the purpose of the 'clock streaking' method when inoculating a solid plated medium?

To obtain isolated colonies (C) Signup and view all the answers

What is the purpose of the 'imprint method' when inoculating a solid plated medium?

To isolate microorganisms from a surface material (C) Signup and view all the answers

Why should mouth-pipetting never be used?

It can lead to contamination of the pipette and the liquid being handled (D) Signup and view all the answers