Laboratory Techniques for Handling Liquid Volumes
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Questions and Answers

What is the purpose of the direct plate exposure method?

  • To flood the surface of a solid medium with a liquid inoculum
  • To spread a liquid inoculum over the surface of a solid medium
  • To sample microorganisms from aerosol or the surrounding air (correct)
  • To distribute the inoculum into the agar medium
  • What is the primary difference between the spread plate and flood plate methods?

  • The spread plate produces a lawn plate, while the flood plate does not
  • The spread plate uses a measured volume of inoculum, while the flood plate uses an unmeasured amount
  • The spread plate inoculates the surface, while the flood plate inoculates the subsurface
  • The spread plate uses a sterile bent glass rod to spread the inoculum, while the flood plate floods the surface with the inoculum (correct)
  • What is the purpose of the pour plate method?

  • To inoculate the surface of a solid medium
  • To inoculate the deeper, microaerobic or anaerobic portions of a solid medium
  • To sample microorganisms from aerosol or the surrounding air
  • To evenly distribute the inoculum throughout the agar medium (correct)
  • When would you use a sterile swab to inoculate an agar plate?

    <p>To sample microorganisms from a dry surface</p> Signup and view all the answers

    What is the purpose of the 'stabbing' inoculation method for solid tube media?

    <p>To inoculate the deeper, microaerobic or anaerobic portions of a butt</p> Signup and view all the answers

    What is the purpose of plugging pipettes with cotton at the region near the aspirator before sterilization?

    <p>To filter out microbial contaminants in the air coming from the aspirator during use</p> Signup and view all the answers

    Which of the following is the correct way to handle used pipettes?

    <p>Immediately place them in a vessel containing disinfectant until they are safe to handle, then wash, sterilize and re-use them</p> Signup and view all the answers

    What is the purpose of the 'clock streaking' method when inoculating a solid plated medium?

    <p>To obtain isolated colonies</p> Signup and view all the answers

    What is the purpose of the 'imprint method' when inoculating a solid plated medium?

    <p>To isolate microorganisms from a surface material</p> Signup and view all the answers

    Why should mouth-pipetting never be used?

    <p>It can lead to contamination of the pipette and the liquid being handled</p> Signup and view all the answers

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