Podcast
Questions and Answers
What is the maximum time a specimen should be delayed before centrifugation to maintain analyte accuracy?
What is the maximum time a specimen should be delayed before centrifugation to maintain analyte accuracy?
- Two hours (correct)
- Four hours
- One hour
- Three hours
Which analyte level could be falsely increased due to prolonged contact with red blood cells?
Which analyte level could be falsely increased due to prolonged contact with red blood cells?
- Glucose
- Bicarbonate
- Potassium (correct)
- Folate
What should be done to maintain specimen integrity if a gel barrier tube is not used?
What should be done to maintain specimen integrity if a gel barrier tube is not used?
- Transfer the serum to another tube after centrifugation (correct)
- Refrigerate the sample immediately
- Keep the sample at room temperature
- Leave the sample undisturbed for four hours
Which tests are negatively affected by pneumatic tube system transport?
Which tests are negatively affected by pneumatic tube system transport?
According to CLSI guidelines, which of the following tests is NOT affected by pneumatic tube system transport?
According to CLSI guidelines, which of the following tests is NOT affected by pneumatic tube system transport?
What type of container must a urine specimen be in when transported via pneumatic tube system?
What type of container must a urine specimen be in when transported via pneumatic tube system?
What action should be taken immediately after centrifugation to ensure test accuracy?
What action should be taken immediately after centrifugation to ensure test accuracy?
What is a potential issue with the pneumatic tube system in specimen transport?
What is a potential issue with the pneumatic tube system in specimen transport?
How does prolonged contact with red blood cells affect potassium levels?
How does prolonged contact with red blood cells affect potassium levels?
What is the main purpose of using a gel barrier in specimen collection?
What is the main purpose of using a gel barrier in specimen collection?
What is the consequence of prolonged plasma or serum contact with blood cells?
What is the consequence of prolonged plasma or serum contact with blood cells?
How quickly should serum or plasma specimens be centrifuged after collection?
How quickly should serum or plasma specimens be centrifuged after collection?
What is the recommended action if testing cannot be completed within eight hours after blood collection?
What is the recommended action if testing cannot be completed within eight hours after blood collection?
Which blood parameters are not affected by light exposure?
Which blood parameters are not affected by light exposure?
What is the effect of glycolysis on glucose levels in blood left at room temperature?
What is the effect of glycolysis on glucose levels in blood left at room temperature?
Which of the following actions should be avoided to prevent specimen contamination during serum collection?
Which of the following actions should be avoided to prevent specimen contamination during serum collection?
Which type of blood specimen can be stored at room temperature for up to 24 hours without adversely affecting results?
Which type of blood specimen can be stored at room temperature for up to 24 hours without adversely affecting results?
What is the maximum time serum or plasma specimens should be frozen for accurate testing?
What is the maximum time serum or plasma specimens should be frozen for accurate testing?
Which of the following statements is true about freezing blood specimens?
Which of the following statements is true about freezing blood specimens?
What should a laboratory do if light-sensitive specimens are collected?
What should a laboratory do if light-sensitive specimens are collected?
What should be checked to ensure the integrity of the carrier for specimen transport?
What should be checked to ensure the integrity of the carrier for specimen transport?
Which of the following test results is generally not affected by pneumatic tube transport?
Which of the following test results is generally not affected by pneumatic tube transport?
What type of label is required for transporting infectious substances via commercial carrier?
What type of label is required for transporting infectious substances via commercial carrier?
What is a recommended action to take before centrifugation of a specimen without anticoagulant?
What is a recommended action to take before centrifugation of a specimen without anticoagulant?
Which test should NOT be transported via a pneumatic tube system according to CLSI recommendations?
Which test should NOT be transported via a pneumatic tube system according to CLSI recommendations?
How long should a specimen be allowed to sit before centrifugation to avoid analyte level interference?
How long should a specimen be allowed to sit before centrifugation to avoid analyte level interference?
What is a consequence of incomplete clotting in a serum specimen?
What is a consequence of incomplete clotting in a serum specimen?
Which of the following recommendations relates to the speed and duration of centrifugation?
Which of the following recommendations relates to the speed and duration of centrifugation?
What happens if a centrifuge is not balanced properly?
What happens if a centrifuge is not balanced properly?
Which of the following analytes can falsely increase due to hemolysis during transport?
Which of the following analytes can falsely increase due to hemolysis during transport?
Study Notes
Pneumatic Tube System for Specimen Transport
- Verify integrity of the carrier before use; check for cracks and ensure closure locks properly.
- Foam pads are essential in the carrier for cushioning and preventing damage during transport.
- Appropriate labels for the transport container include biohazard, "Biological Substance Category A," or "Biological Substance Category B," depending on the infectious material.
Impact of Transportation on Test Results
- Albumin test results remain unaffected by pneumatic tube system transport.
- Certain tests are negatively affected due to red blood cell disruption, including potassium, plasma hemoglobin, acid phosphatase, and lactate dehydrogenase, as recommended by CLSI.
- Tests not influenced by pneumatic tube transport include albumin, alkaline phosphatase, AST, chloride, creatinine, glucose, sodium, total bilirubin, total protein, BUN, uric acid, thrombin time, and WBC concentration.
Specimen Handling Prior to Centrifugation
- Allow specimens without additives to clot for at least 30 minutes before centrifugation to avoid fibrin residues.
- Specimens should not be held for over two hours prior to centrifugation to prevent analyte level inaccuracies, including falsely decreased glucose and increased potassium due to hemolysis.
- Centrifugation should be properly balanced to prevent tube breakage and biohazard exposure.
Time Constraints for Serum/Plasma
- Specimens should be centrifuged within two hours to prevent false alterations in analyte levels caused by red blood cell contact.
- Continuous contact between serum and red blood cells can lead to significant potassium level increases.
Urine Specimen Transport
- To prevent leakage, transport urine specimens in leak-proof primary receptacles with tightly secured lids, placed inside secondary containers like biohazard bags.
Blood Ammonia Handling
- Blood ammonia specimens must be kept in an ice slurry and processed quickly to avoid increases from in vitro amino acid breakdown.
Blood Specimen Storage Guidelines
- Centrifugation of serum/plasma should occur within two hours to avoid cellular changes and altered analyte levels.
- Avoid rimming clots in serum tubes to prevent aerosol contamination and hemolysis.
- Blood glucose can decrease by 5%-7% per hour due to glycolysis if stored at room temperature.
Storage Conditions for Specimens
- EDTA whole blood tubes for CBCs can be stored at room temperature for up to 24 hours and refrigerated for 48 hours.
- Serum/plasma should not be at room temperature for extended periods; if testing is delayed over 8 hours, refrigerate at 2-8°C; if over 48 hours, freeze at -20°C or below.
- Frozen specimens should be thawed only once before testing to maintain analyte integrity.
Light Sensitivity of Specimens
- Vitamin B12, bilirubin, beta carotene, and folate are light-sensitive and should be stored in amber tubes or wrapped in aluminum foil.
- Other analytes like 03C, PT, creatinine, magnesium, and potassium are not affected by light exposure.
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Description
This quiz focuses on the best practices for transporting laboratory specimens via pneumatic tube systems. Participants will learn about the necessary checks for carrier integrity, cushioning requirements, and labeling protocols to ensure safe delivery. Understanding these guidelines is crucial for maintaining the quality and safety of clinical specimens.
