Laboratory Safety Guidelines Quiz

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

What is the protocol for students who display COVID-19 symptoms before arriving at the lab?

They must report immediately and seek medical help. (correct)

They should attend class after notifying their friends.

They should participate synchronously online without permission.

They are allowed in the lab if they wear a mask.

Which of the following pieces of personal protective equipment must be worn by students in the lab?

Lab coat, goggles, and gloves. (correct)

Safety boots and a hat.

Only a mask and gloves.

Short sleeves for comfort.

What is the maximum number of justified absences a student can have?

Unlimited justified absences with prior notice.

One absence for health issues.

Two justified absences. (correct)

Three excused absences with valid reasons.

Which statement is true regarding lab experiment repetitions?

Lab experiments are not to be repeated. Signup and view all the answers

How should students maintain hygiene during lab work?

By washing hands with soap and disinfecting surfaces before and after. Signup and view all the answers

What is an important guideline regarding communication in the lab?

Raise your hand to ask questions. Signup and view all the answers

What calculations need to be made before conducting an experiment?

Concentration calculations using $C1.V1=C2.V2$. Signup and view all the answers

What should students do to prepare for lab exercises?

Review and print the experimental methodology in advance. Signup and view all the answers

What is the primary function of the Pipetman in a laboratory setting?

To measure liquid volumes accurately Signup and view all the answers

Which Pipetman model would be best suited for transferring 50 μl of a liquid?

P200 Signup and view all the answers

How can you verify the accuracy of a Pipetman?

By weighing water to confirm volume corresponds to weight Signup and view all the answers

In biochemistry, what term describes the substance dissolved in a solution?

Solute Signup and view all the answers

What is the density of water at room temperature relevant for in the context of pipetting?

To verify the mass of liquid pipetted corresponds to volume Signup and view all the answers

What is the concentration expressed as % by mass when dissolving 5 g of NaOH into 100 g of solution?

5% Signup and view all the answers

Which statement correctly defines an aqueous solution?

A solution where water serves as the solvent Signup and view all the answers

Which of the following equipment is most appropriate for preparing dilutions in a laboratory?

Pipetman Signup and view all the answers

Study Notes

Course Information

Course: Medical Biochemistry I
Lab Exercise 1: Introduction to Techniques - Use of Pipetman, Sample Preparation and Dilutions
Semester: Fall 2024
Instructors: Andreou M & Kyriakou TC
Institution: European University Cyprus, School of Medicine

Objectives

Introduction: Lab characteristics/guidelines
Part A: Use of Pipetman (theoretical background and exercises)
Part B: Sample Preparation (main units of concentration and exercises)
Part C: Dilutions (dilution formula and exercises)

COVID-19 Safety Protocol

Be on time to avoid crowds at the lab entrance.
Always bring the required protective gear (lab coat with cuffed sleeves, mask, and goggles).
Students without the necessary gear will not be permitted in the lab.
Strict hand hygiene is required (wash hands with soap and put on gloves).
Disinfect working surfaces before and after experiments.
Report any symptoms (fever, cough, or breathing difficulties) immediately.

Absences

Mandatory participation (physical presence or synchronous participation; permission required).
Only two justified absences are allowed (severe health conditions or emergencies).
Pre-announce any justified absences before class.
Lab experiments cannot be repeated.

Preparation for Lab

Study uploaded materials.
Print the protocol/experimental methodology.
Identify required materials and equipment from the protocol.
Be mentally prepared for technically demanding tasks.
Wear closed-toe shoes, lab coat, goggles, and gloves.
Keep hair tied back (ponytail).
No food or drinks allowed.

Laboratory Notebook

Record everything explained during the lab.
Document protocol applications with dates.
Calculate reagent concentrations (e.g., C1V1=C2V2).
Evaluate results and identify areas for improvement.

Lab Conduct

Move cautiously (as if in a bubble).
Be aware of your surroundings.
Work as a team.
Show respect to others.
Ask questions by raising your hand.
Avoid fast movements.
Work quietly.

Contact Information

Email: [email protected] & [email protected]
Office: N30 (2nd floor - Medical Building)
EUC email account policy: Faculty communication through personal email should be notified as it must be sent through EUC email account.

Materials and Equipment

Pipetman (P1000, P200, P10)
Pipet tips
Distilled Water
1.0 M CuSO4 solution
Cuvettes
Balance machine
Spectrophotometer
Weighing boats
Waste containers

Pipetman Use

Pipetman is used for volumes under 1 ml. This is a common tool in labs.
Available in three types
- P1000 (200-1000 µl).
- P100 (10-100 µl).
- P10 (0.5-10 µl).
Proper use of micropipettes involves recognizing the upper/first/second stops for accurate volumes.

Pipette Accuracy

The most common method for determining pipette accuracy is weighing water.
Water density at room temperature is approximately 1 g/ml. Therefore, 1 µl of water should weigh 0.001 grams

Part A: Use of 1000µl and 200µl Pipettman

Instructions to measure accurately various volumes of distilled water for each Pipetman using the correct procedures.

Part B: Sample Preparation

A solvent is the liquid that makes up a solution. Water is a common solvent, and solutions formed with water are referred to as aqueous solutions.
A solute is the material dissolved in the solvent.
Solution concentration is the amount of solute per unit of solution. Concentration can be expressed as Percentage by Mass (w/w), Percentage by volume (v/v), Percentage weight by volume (w/v), and Molarity (M).

Part B: Calculations and Concentration

Methods for calculating various concentration units of common solutions (e.g., % by mass, % by volume, % weight / volume and Molarity of common solutions such as NaOH solutions)

Part C: Dilutions

Most biochemical solutions are prepared by diluting stock solutions.
The formula C1V1 = C2V2 is used to calculate dilutions, where:
- C1 = Initial concentration
- V1 = Initial volume
- C2 = Final concentration
- V2 = Final volume
Dilutions may use concentration factors (10X, 20X, etc). Dilution is carried out before use.

Studying That Suits You

Use AI to generate personalized quizzes and flashcards to suit your learning preferences.

Description

Test your knowledge on the essential safety protocols and guidelines for students in the laboratory. This quiz covers topics like personal protective equipment, hygiene practices, justifiable absences, and communication during lab exercises. Ensure you're well-prepared to maintain a safe and effective lab environment!