Podcast
Questions and Answers
Which solute has the highest partition coefficient, indicating the greatest nonpolarity?
Which solute has the highest partition coefficient, indicating the greatest nonpolarity?
- Solute D
- Solute B
- Solute A (correct)
- Solute C
In a typical experimental design, which of the following correctly identifies the independent variable?
In a typical experimental design, which of the following correctly identifies the independent variable?
- Temperature variations during the experiment (correct)
- The type of apples being tested
- The amount of microorganisms present
- The growth rate of microbes
What is the correct order of solutes from fastest to slowest lysis time according to their partition coefficients?
What is the correct order of solutes from fastest to slowest lysis time according to their partition coefficients?
- A, C, D, B (correct)
- C, B, D, A
- A, B, C, D
- B, C, A, D
Which of the following statements best describes the difference between a research question and a hypothesis?
Which of the following statements best describes the difference between a research question and a hypothesis?
Which of the following solutes would likely have the slowest lysis time based on partition coefficients?
Which of the following solutes would likely have the slowest lysis time based on partition coefficients?
What is the primary use of a serological pipette compared to a Pasteur pipette?
What is the primary use of a serological pipette compared to a Pasteur pipette?
What is the total magnification achieved when using a 40X objective lens with a 10X eyepiece on a compound microscope?
What is the total magnification achieved when using a 40X objective lens with a 10X eyepiece on a compound microscope?
When using a serological pipette, which of the following statements is true?
When using a serological pipette, which of the following statements is true?
What potential issue could cause a positive result in the negative control during an iodine test?
What potential issue could cause a positive result in the negative control during an iodine test?
Which pipetting equipment can determine an exact volume dispensed?
Which pipetting equipment can determine an exact volume dispensed?
While using the fine focus knob on a microscope, what is the main reason for avoiding the coarse focus at high magnification?
While using the fine focus knob on a microscope, what is the main reason for avoiding the coarse focus at high magnification?
How does a serological pipette differ fundamentally in operation from a micropipette?
How does a serological pipette differ fundamentally in operation from a micropipette?
Why is it important to measure the volume indicated on a serological pipette correctly?
Why is it important to measure the volume indicated on a serological pipette correctly?
Which micropipette setting would you use to dispense 150 μL of solution?
Which micropipette setting would you use to dispense 150 μL of solution?
What is the first step in using a micropipette to dispense liquid?
What is the first step in using a micropipette to dispense liquid?
Which of the following steps ensures that the liquid is fully dispensed from the micropipette?
Which of the following steps ensures that the liquid is fully dispensed from the micropipette?
What is the correct conversion of 150 μL to mL?
What is the correct conversion of 150 μL to mL?
What is the purpose of the tip ejector when using a micropipette?
What is the purpose of the tip ejector when using a micropipette?
What causes the lysis of red blood cells (RBCs) in certain test solutions?
What causes the lysis of red blood cells (RBCs) in certain test solutions?
Why is a negative control important in the lysis time experiment using temperatures?
Why is a negative control important in the lysis time experiment using temperatures?
If a solution has a concentration of 0.7 M NaCl, what is the total concentration of solute particles in that solution?
If a solution has a concentration of 0.7 M NaCl, what is the total concentration of solute particles in that solution?
What conclusion can be drawn about the permeability of the decalcified egg membrane when it swelled in Test Solution A?
What conclusion can be drawn about the permeability of the decalcified egg membrane when it swelled in Test Solution A?
What result would indicate that high temperature caused cell denaturation rather than increased membrane permeability?
What result would indicate that high temperature caused cell denaturation rather than increased membrane permeability?
What is a standardized variable in an experiment?
What is a standardized variable in an experiment?
Which of the following best describes a confounding variable?
Which of the following best describes a confounding variable?
In the context of a given research question, what does the hypothesis represent?
In the context of a given research question, what does the hypothesis represent?
What would be the independent variable in an experiment measuring flowers' growth on the hiking trail versus the running track?
What would be the independent variable in an experiment measuring flowers' growth on the hiking trail versus the running track?
In using a micropipette to dispense a specific volume, what does the P200 setting indicate?
In using a micropipette to dispense a specific volume, what does the P200 setting indicate?
What is the primary reason for using proper controls in an experimental design?
What is the primary reason for using proper controls in an experimental design?
What is the correct volume in milliliters when converting 150 L?
What is the correct volume in milliliters when converting 150 L?
Which statement accurately describes the purpose of a flowchart in documenting experimental design?
Which statement accurately describes the purpose of a flowchart in documenting experimental design?
Flashcards
Partition Coefficient
Partition Coefficient
A measure of a solute's relative nonpolarity, indicating faster lysis time with higher values.
Independent Variable
Independent Variable
The variable manipulated by the researcher in an experiment.
Dependent Variable
Dependent Variable
The variable measured to see how it changes in response to the independent variable.
Research Question
Research Question
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Hypothesis vs Research Question
Hypothesis vs Research Question
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Serological Pipette
Serological Pipette
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Pasteur Pipette
Pasteur Pipette
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Micropipette
Micropipette
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Compound Microscope
Compound Microscope
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Total Magnification
Total Magnification
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Iodine Test
Iodine Test
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Negative Control
Negative Control
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How does the iodine test work?
How does the iodine test work?
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P200 Micropipette
P200 Micropipette
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P1000 Micropipette
P1000 Micropipette
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How to set the micropipette
How to set the micropipette
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Microliter (μL) to Milliliter (mL) conversion
Microliter (μL) to Milliliter (mL) conversion
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Standardized Variable
Standardized Variable
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Confounding Variable
Confounding Variable
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Micropipette Setting
Micropipette Setting
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Hypothesis
Hypothesis
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Experimental Design
Experimental Design
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Lysis
Lysis
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Osmosis
Osmosis
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Isotonic Solution
Isotonic Solution
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Hypotonic Solution
Hypotonic Solution
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Study Notes
200 Question
- Name one piece of equipment used in lab and explain how it is used
- Examples: Serological pipette, Pasteur pipette, spectrophotometer, compound microscope, Eppendorf microcentrifuge
- Serological pipette: used for large volumes
- Pasteur pipette: used for smaller volumes
400 Question
- Explain why a serological pipette is used over a Pasteur pipette
- Serological pipette: used for large volumes needing precise measurements
- Pasteur pipette: used for smaller volumes where precise measurements are less important
600 Question
- Determine the total volume in a given pipette, explain how
- Total volume is determined using the markings on the pipette
- The markings indicate the increments of liquid dispensed (ex: 1/10).
- Each minor tick represents a specific volume increment (ex: 0.1 mL)
800 Question
- Explain why you would use the 40x objective on a compound microscope
- Use the 40x objective when needing higher magnification.
- Use the fine focus when using the 40x objective to adjust the image for clarity
- Coarse focus should not be used with higher magnification objectives as this can damage the slide and lense.
1000 Question
- Determine the order to place RBCs in different solutions to cross a BC membrane.
- Order solutions by partition coefficient, from lowest to highest
- In the table, a higher partition coefficient indicates that the solute will be more quickly to the membrane.
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