Laboratory Equipment Quiz

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Questions and Answers

Which solute has the highest partition coefficient, indicating the greatest nonpolarity?

Solute D

Solute B

Solute A (correct)

Solute C

In a typical experimental design, which of the following correctly identifies the independent variable?

Temperature variations during the experiment (correct)

The type of apples being tested

The amount of microorganisms present

The growth rate of microbes

What is the correct order of solutes from fastest to slowest lysis time according to their partition coefficients?

A, C, D, B (correct)

C, B, D, A

A, B, C, D

B, C, A, D

Which of the following statements best describes the difference between a research question and a hypothesis?

A research question seeks to understand relationships, while a hypothesis includes a prediction and rationale. Signup and view all the answers

Which of the following solutes would likely have the slowest lysis time based on partition coefficients?

Solute B Signup and view all the answers

What is the primary use of a serological pipette compared to a Pasteur pipette?

For measuring and transferring large volumes accurately Signup and view all the answers

What is the total magnification achieved when using a 40X objective lens with a 10X eyepiece on a compound microscope?

400x Signup and view all the answers

When using a serological pipette, which of the following statements is true?

It can be used to measure volumes greater than 10 mL. Signup and view all the answers

What potential issue could cause a positive result in the negative control during an iodine test?

The iodine solution is contaminated with starch. Signup and view all the answers

Which pipetting equipment can determine an exact volume dispensed?

Serological pipette Signup and view all the answers

While using the fine focus knob on a microscope, what is the main reason for avoiding the coarse focus at high magnification?

It could potentially damage the specimen or lens. Signup and view all the answers

How does a serological pipette differ fundamentally in operation from a micropipette?

It requires manual suction for liquid intake. Signup and view all the answers

Why is it important to measure the volume indicated on a serological pipette correctly?

To maintain consistency in experimental conditions. Signup and view all the answers

Which micropipette setting would you use to dispense 150 μL of solution?

P200 set to 150 Signup and view all the answers

What is the first step in using a micropipette to dispense liquid?

Attach the pipette tip to the pipette. Signup and view all the answers

Which of the following steps ensures that the liquid is fully dispensed from the micropipette?

Push the plunger to the second stop. Signup and view all the answers

What is the correct conversion of 150 μL to mL?

0.150 mL Signup and view all the answers

What is the purpose of the tip ejector when using a micropipette?

To dispose of the used pipette tip safely. Signup and view all the answers

What causes the lysis of red blood cells (RBCs) in certain test solutions?

Water enters the cell due to osmosis following the permeable solutes. Signup and view all the answers

Why is a negative control important in the lysis time experiment using temperatures?

To ensure that lysis is only due to membrane permeability and not temperature effects. Signup and view all the answers

If a solution has a concentration of 0.7 M NaCl, what is the total concentration of solute particles in that solution?

1.4 M Signup and view all the answers

What conclusion can be drawn about the permeability of the decalcified egg membrane when it swelled in Test Solution A?

The membrane is semi-permeable and allows some solutes to enter. Signup and view all the answers

What result would indicate that high temperature caused cell denaturation rather than increased membrane permeability?

Lysis occurred in all tested solutions regardless of concentration. Signup and view all the answers

What is a standardized variable in an experiment?

A variable that is kept constant to assess the impact on the dependent variable. Signup and view all the answers

Which of the following best describes a confounding variable?

A variable that can influence the dependent variable and is not controlled. Signup and view all the answers

In the context of a given research question, what does the hypothesis represent?

A specific prediction about the relationship between variables. Signup and view all the answers

What would be the independent variable in an experiment measuring flowers' growth on the hiking trail versus the running track?

The location of the flowers (hiking trail or running track). Signup and view all the answers

In using a micropipette to dispense a specific volume, what does the P200 setting indicate?

It can accurately dispense volumes from 0.2 µL to 200 µL. Signup and view all the answers

What is the primary reason for using proper controls in an experimental design?

To isolate the effects of the independent variable on the dependent variable. Signup and view all the answers

What is the correct volume in milliliters when converting 150 L?

150,000 mL Signup and view all the answers

Which statement accurately describes the purpose of a flowchart in documenting experimental design?

It visually represents the step-by-step process of the experiment. Signup and view all the answers

Study Notes

200 Question

Name one piece of equipment used in lab and explain how it is used
Examples: Serological pipette, Pasteur pipette, spectrophotometer, compound microscope, Eppendorf microcentrifuge
Serological pipette: used for large volumes
Pasteur pipette: used for smaller volumes

400 Question

Explain why a serological pipette is used over a Pasteur pipette
Serological pipette: used for large volumes needing precise measurements
Pasteur pipette: used for smaller volumes where precise measurements are less important

600 Question

Determine the total volume in a given pipette, explain how
Total volume is determined using the markings on the pipette
The markings indicate the increments of liquid dispensed (ex: 1/10).
Each minor tick represents a specific volume increment (ex: 0.1 mL)

800 Question

Explain why you would use the 40x objective on a compound microscope
Use the 40x objective when needing higher magnification.
Use the fine focus when using the 40x objective to adjust the image for clarity
Coarse focus should not be used with higher magnification objectives as this can damage the slide and lense.

1000 Question

Determine the order to place RBCs in different solutions to cross a BC membrane.
Order solutions by partition coefficient, from lowest to highest
In the table, a higher partition coefficient indicates that the solute will be more quickly to the membrane.

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Description

Test your knowledge on laboratory equipment, specifically pipettes and microscopes. This quiz covers their uses, advantages, and how to measure volumes accurately. Perfect for students in biology or chemistry classes!