Laboratory Equipment Quiz

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Questions and Answers

Which solute has the highest partition coefficient, indicating the greatest nonpolarity?

  • Solute D
  • Solute B
  • Solute A (correct)
  • Solute C

In a typical experimental design, which of the following correctly identifies the independent variable?

  • Temperature variations during the experiment (correct)
  • The type of apples being tested
  • The amount of microorganisms present
  • The growth rate of microbes

What is the correct order of solutes from fastest to slowest lysis time according to their partition coefficients?

  • A, C, D, B (correct)
  • C, B, D, A
  • A, B, C, D
  • B, C, A, D

Which of the following statements best describes the difference between a research question and a hypothesis?

<p>A research question seeks to understand relationships, while a hypothesis includes a prediction and rationale. (A)</p> Signup and view all the answers

Which of the following solutes would likely have the slowest lysis time based on partition coefficients?

<p>Solute B (C)</p> Signup and view all the answers

What is the primary use of a serological pipette compared to a Pasteur pipette?

<p>For measuring and transferring large volumes accurately (C)</p> Signup and view all the answers

What is the total magnification achieved when using a 40X objective lens with a 10X eyepiece on a compound microscope?

<p>400x (C)</p> Signup and view all the answers

When using a serological pipette, which of the following statements is true?

<p>It can be used to measure volumes greater than 10 mL. (C)</p> Signup and view all the answers

What potential issue could cause a positive result in the negative control during an iodine test?

<p>The iodine solution is contaminated with starch. (C)</p> Signup and view all the answers

Which pipetting equipment can determine an exact volume dispensed?

<p>Serological pipette (A)</p> Signup and view all the answers

While using the fine focus knob on a microscope, what is the main reason for avoiding the coarse focus at high magnification?

<p>It could potentially damage the specimen or lens. (D)</p> Signup and view all the answers

How does a serological pipette differ fundamentally in operation from a micropipette?

<p>It requires manual suction for liquid intake. (C)</p> Signup and view all the answers

Why is it important to measure the volume indicated on a serological pipette correctly?

<p>To maintain consistency in experimental conditions. (D)</p> Signup and view all the answers

Which micropipette setting would you use to dispense 150 μL of solution?

<p>P200 set to 150 (A)</p> Signup and view all the answers

What is the first step in using a micropipette to dispense liquid?

<p>Attach the pipette tip to the pipette. (D)</p> Signup and view all the answers

Which of the following steps ensures that the liquid is fully dispensed from the micropipette?

<p>Push the plunger to the second stop. (C)</p> Signup and view all the answers

What is the correct conversion of 150 μL to mL?

<p>0.150 mL (D)</p> Signup and view all the answers

What is the purpose of the tip ejector when using a micropipette?

<p>To dispose of the used pipette tip safely. (D)</p> Signup and view all the answers

What causes the lysis of red blood cells (RBCs) in certain test solutions?

<p>Water enters the cell due to osmosis following the permeable solutes. (A)</p> Signup and view all the answers

Why is a negative control important in the lysis time experiment using temperatures?

<p>To ensure that lysis is only due to membrane permeability and not temperature effects. (C)</p> Signup and view all the answers

If a solution has a concentration of 0.7 M NaCl, what is the total concentration of solute particles in that solution?

<p>1.4 M (B)</p> Signup and view all the answers

What conclusion can be drawn about the permeability of the decalcified egg membrane when it swelled in Test Solution A?

<p>The membrane is semi-permeable and allows some solutes to enter. (A)</p> Signup and view all the answers

What result would indicate that high temperature caused cell denaturation rather than increased membrane permeability?

<p>Lysis occurred in all tested solutions regardless of concentration. (B)</p> Signup and view all the answers

What is a standardized variable in an experiment?

<p>A variable that is kept constant to assess the impact on the dependent variable. (A)</p> Signup and view all the answers

Which of the following best describes a confounding variable?

<p>A variable that can influence the dependent variable and is not controlled. (C)</p> Signup and view all the answers

In the context of a given research question, what does the hypothesis represent?

<p>A specific prediction about the relationship between variables. (C)</p> Signup and view all the answers

What would be the independent variable in an experiment measuring flowers' growth on the hiking trail versus the running track?

<p>The location of the flowers (hiking trail or running track). (B)</p> Signup and view all the answers

In using a micropipette to dispense a specific volume, what does the P200 setting indicate?

<p>It can accurately dispense volumes from 0.2 µL to 200 µL. (A)</p> Signup and view all the answers

What is the primary reason for using proper controls in an experimental design?

<p>To isolate the effects of the independent variable on the dependent variable. (B)</p> Signup and view all the answers

What is the correct volume in milliliters when converting 150 L?

<p>150,000 mL (D)</p> Signup and view all the answers

Which statement accurately describes the purpose of a flowchart in documenting experimental design?

<p>It visually represents the step-by-step process of the experiment. (D)</p> Signup and view all the answers

Flashcards

Partition Coefficient

A measure of a solute's relative nonpolarity, indicating faster lysis time with higher values.

Independent Variable

The variable manipulated by the researcher in an experiment.

Dependent Variable

The variable measured to see how it changes in response to the independent variable.

Research Question

Asks how two or more factors relate, without a prediction.

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Hypothesis vs Research Question

Hypothesis includes a prediction & rationale while a research question focuses on understanding relationships between factors.

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Serological Pipette

A graduated glass tube used for precise dispensing of large volumes of liquids, typically ranging from 1 mL to 50 mL.

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Pasteur Pipette

A disposable glass tube used for transferring small volumes of liquids, usually without precise measurement.

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Micropipette

A precision instrument used to dispense very small volumes of liquids, typically in microliters (µL).

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Compound Microscope

A type of microscope that uses multiple lenses to magnify an object, providing a 3D image with high resolution.

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Total Magnification

The overall magnification power of a compound microscope, calculated by multiplying the magnification of the objective lens by the magnification of the eyepiece lens.

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Iodine Test

A simple chemical test used to detect the presence of starch in a sample.

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Negative Control

A sample that should not contain the substance being tested for, used to ensure that the test is working correctly.

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How does the iodine test work?

Iodine solution interacts with starch molecules, causing a blue-black color change. The reaction occurs due to the formation of a complex between iodine and the amylose component of starch.

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P200 Micropipette

A type of micropipette capable of dispensing volumes between 20 and 200 μL. It has a three-digit display, where the first digit indicates the hundreds place and the second digit indicates the tens place.

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P1000 Micropipette

A type of micropipette capable of dispensing volumes between 100 and 1000 μL. It has a four-digit display, where the first digit indicates the hundreds place, the second digit indicates the tens place, and the third digit indicates the ones place.

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How to set the micropipette

To set a micropipette for a specific volume, rotate the volume adjustment knob to the desired value. For P200, set the volume to 150μL using the three-digit display (150). For P1000, set the volume to 150μL using the four-digit display (0150).

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Microliter (μL) to Milliliter (mL) conversion

One milliliter (mL) is equal to 1000 microliters (μL). To convert μL to mL, divide the volume in μL by 1000.

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Standardized Variable

A variable that is kept constant throughout an experiment to ensure consistent conditions across different groups or treatments.

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Confounding Variable

A variable that unexpectedly influences the results of an experiment, making it difficult to determine the true effect of the independent variable.

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Micropipette Setting

The numerical volume displayed on the micropipette, indicating the amount of liquid to be dispensed.

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Hypothesis

A testable prediction that suggests a specific relationship between variables, often based on existing knowledge or observations.

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Experimental Design

A structured plan outlining the steps involved in conducting an experiment, including the independent and dependent variables, control groups, and the methods for data collection.

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Lysis

The bursting of a cell, often due to an influx of water caused by osmotic pressure differences.

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Osmosis

The movement of water across a semi-permeable membrane from an area of high water concentration to an area of low water concentration.

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Isotonic Solution

A solution with the same solute concentration as the inside of a cell, resulting in no net movement of water across the cell membrane.

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Hypotonic Solution

A solution with a lower solute concentration than the inside of a cell, causing water to move into the cell and potentially causing it to swell or lyse.

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Study Notes

200 Question

  • Name one piece of equipment used in lab and explain how it is used
  • Examples: Serological pipette, Pasteur pipette, spectrophotometer, compound microscope, Eppendorf microcentrifuge
  • Serological pipette: used for large volumes
  • Pasteur pipette: used for smaller volumes

400 Question

  • Explain why a serological pipette is used over a Pasteur pipette
  • Serological pipette: used for large volumes needing precise measurements
  • Pasteur pipette: used for smaller volumes where precise measurements are less important

600 Question

  • Determine the total volume in a given pipette, explain how
  • Total volume is determined using the markings on the pipette
  • The markings indicate the increments of liquid dispensed (ex: 1/10).
  • Each minor tick represents a specific volume increment (ex: 0.1 mL)

800 Question

  • Explain why you would use the 40x objective on a compound microscope
  • Use the 40x objective when needing higher magnification.
  • Use the fine focus when using the 40x objective to adjust the image for clarity
  • Coarse focus should not be used with higher magnification objectives as this can damage the slide and lense.

1000 Question

  • Determine the order to place RBCs in different solutions to cross a BC membrane.
  • Order solutions by partition coefficient, from lowest to highest
  • In the table, a higher partition coefficient indicates that the solute will be more quickly to the membrane.

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