Podcast
Questions and Answers
What is the name of the protein that is lacking in Duchenne muscular dystrophy?
What is the name of the protein that is lacking in Duchenne muscular dystrophy?
- Dystrophin (correct)
- Hassan
- Duchenne
- Rasha
Why are Duchenne muscular dystrophy and Becker muscular dystrophy X-linked recessive disorders?
Why are Duchenne muscular dystrophy and Becker muscular dystrophy X-linked recessive disorders?
- Because the gene is on a mitochondrial chromosome
- Because the gene is on the Y chromosome
- Because the gene is on a non-sex chromosome
- Because the gene is on the X chromosome (correct)
At what age does Duchenne muscular dystrophy typically start showing symptoms?
At what age does Duchenne muscular dystrophy typically start showing symptoms?
- In adulthood, around age 20
- In infancy
- In late childhood, around age 10
- In early childhood, usually between ages 2 and 3 (correct)
What percentage of patients with DMD or BMD have a deletion of one or more exons?
What percentage of patients with DMD or BMD have a deletion of one or more exons?
How many exons can be amplified simultaneously using multiplex PCR?
How many exons can be amplified simultaneously using multiplex PCR?
What is the purpose of agarose gel electrophoresis in the diagnosis of DMD/BMD?
What is the purpose of agarose gel electrophoresis in the diagnosis of DMD/BMD?
What is the initial temperature set for the thermal cycler?
What is the initial temperature set for the thermal cycler?
How many cycles are performed in the PCR reaction?
How many cycles are performed in the PCR reaction?
What is the purpose of the negative control in the PCR reaction?
What is the purpose of the negative control in the PCR reaction?
What is the concentration of ethidium bromide in the TBE running buffer?
What is the concentration of ethidium bromide in the TBE running buffer?
What is the purpose of the final extension period in the PCR reaction?
What is the purpose of the final extension period in the PCR reaction?
How long does the electrophoresis take to complete?
How long does the electrophoresis take to complete?
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