29 Questions
Which method of microscopy has the highest magnification?
Transmission electron microscopy
Which method of microscopy has the highest optical resolution?
Transmission electron microscopy
What is the separation distance at which two objects in an image can be apart and still distinguished as separate?
Resolution
What is the resolution of a microscope with a resolution of 200nm?
200nm
Which method of microscopy is used for 2D images of electron beams scattered from the surface of a sample?
Scanning electron microscopy
Which method of microscopy is used for 3D images of photoreceptors in the retina?
Transmission electron microscopy
What is the purpose of staining with dyes in histology?
To visualize tissue structures
What is the main purpose of fixation in histology?
To preserve cells/tissue
Which method is commonly used to embed fixed tissues in histology?
All of the above
What is the purpose of staining with Haematoxylin and Eosin (H&E) in histology?
To visualize tissue structures
What does immunohistochemistry use to label a specific protein or cell?
Antibodies
Which of the following is a characteristic of dysplasic colon?
All of the above
What is the composition of normal prostate?
40% basal cells & 60% luminal cells
What is a characteristic of prostate cancer?
Luminal hyperproliferation
What is a characteristic of benign tumors?
They have clear boundaries
Which legislation regulates the removal, storage, use, and disposal of human tissue?
Human Tissue Act (2004)
What is the fundamental principle of the Human Tissue Act (2004)?
Consent for removal, storage, and use of human tissue
What is the composition of luminal cells in prostate tumors?
99%
What is the composition of basal cells in prostate tumors?
< 0.1%
True or false: Most cells are visible to the naked eye?
False
True or false: The resolution of an electron microscope can be as low as 0.2nm?
True
True or false: The separation distance at which two objects in an image can be distinguished as separate is called optical resolution?
True
True or false: Fixation in histology prevents tissue degradation and preserves biological activity?
True
True or false: Staining with Haematoxylin in histology allows for visualization of acidic structures?
True
True or false: Immunohistochemistry uses antibodies to label specific proteins or cells?
True
True or false: Dysplasic colon is characterized by irregularity of crypt contour.
True
True or false: Prostate tumors are composed of > 99% basal cells.
False
True or false: Benign tumors have the potential to grow into surrounding tissues.
False
True or false: The Human Tissue Act (2004) requires consent for the removal, storage, and use of human tissue.
True
Test your knowledge of histology and cytology with this quiz! Explore the microscopic study of tissues and cells, from organs to neurons, and uncover the fascinating world that lies beyond the naked eye.
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