39 Questions
Which technique is used to quantify enzymes via immunoreactivity?
Immunochemical analysis
What is the principle behind Reverse Genomics?
Working backwards from observed phenotypes to identify the underlying genetic basis
Which technique is used for purification of microbial isolates?
Fluorescence-activated cell sorting (FACS)
Which microscopy technique uses dyes for easier and more specific visualization of microorganisms?
Gram staining
Which molecular technique is used for rapid amplification of nucleic acids?
PCR
Which technique is used for screening microorganisms with bioactivity?
Chromogenic Media
Which molecular technique is used for identifying diverse range of bacterial species?
RAPD-PCR
Which biochemical analytical method is used for analysis of non-volatile or thermally labile high molecular compounds?
HPLC-based methods
Which of the following is a technique used for separating microorganisms based on their size and density?
Differential centrifugation
Which of the following staining techniques is commonly used to differentiate between gram-positive and gram-negative bacteria?
Gram staining
What is the primary purpose of using lactophenol blue staining in microscopy?
To stain fungal chitin cell walls
Which of the following is an example of a direct assay for screening microorganisms with bioactivity?
Absorbance spectroscopy
What is the primary purpose of using a moist chamber culture during the isolation and purification of microorganisms?
To maintain a high humidity environment for microbial growth
Which of the following techniques is used to separate microorganisms based on their ability to form colonies on a solid growth medium?
Agar plating
Which of the following assays is used to determine the minimum inhibitory concentration (MIC) of an antimicrobial agent against a microorganism?
Broth dilution method
What is the primary purpose of using a time-kill test or time-kill curve assay during the screening of microorganisms with bioactivity?
To determine the bactericidal or fungicidal effect of an antimicrobial agent over time
What is the main purpose of selective media in microbiology?
To encourage the growth of specific microorganisms while inhibiting others
What technique is used to reduce the number of microbial species for plating techniques?
Serial Dilution Method
Which method involves atomizing a liquid cell suspension with sterile air to scatter cells onto an agar plate?
Cell Spray
What is the disadvantage of spread plating as a culture-dependent method?
Formation of coalesced colonies
Which type of media is supplemented with nutritious materials to encourage the growth of a specific group of microorganisms?
Enriched media
What is the purpose of differential media in microbiology?
To visually distinguish between different types of microorganisms
Which method allows for the separation of particulate contents based on size and shape?
"Filtration"
Which of the following techniques allows for the cultivation of microbial cells requiring interspecific/intraspecific interactions?
Hollow-fiber membrane chamber (HFMC)
What is the principle behind the Reverse Genomics technique?
Utilization of engineered genome-informed antibody to capture specific microorganisms from complex microbial communities
Which technique is commonly combined with fluorescence-activated cell sorting (FACS) for cell sorting?
Live-Fluorescent in situ hybridization (FISH)
Which technique involves the inoculation of a mix of environmental cells and the diffusion of nutrients from the environment?
Diffusion chamber
What is the purpose of the iChip technique?
To produce pure cultures by isolating single cells in individual chambers
Which microscopy technique is used to stain and observe bacterial cell walls?
Gram staining
Which technique involves the use of a dye to stain and observe fungal structures?
Lactophenol Blue Staining
Which technique is used to screen for microorganisms with bioactive compounds?
Bioassay screening
Which technique is used to sort cells based on their specific light scattering and fluorescent characteristics?
Fluorescence-activated cell sorting (FACS)
Which technique is commonly used for photophysiological studies of phytoplankton?
Optical Tweezers
Which technique is used for the isolation of flagellates that exhibit phototaxis?
Phototaxis
Which technique is used for single-cell isolation while avoiding cell damage?
Micropipette
Which technique is used for the separation of cells according to size and density, and the concentration of larger cells?
Polycarbonate filter Centrifugation
Which technique is used to count and analyze the optical properties of cells?
Flow cytometry
Which microscopy technique involves staining cells with a blue dye to enhance the visibility of certain cellular structures?
Lactophenol Blue Staining
Which technique is used to screen microorganisms for bioactive compounds or enzymes?
Reverse Genomics Principle
Explore various methods used in microbiology for isolating and purifying microorganisms, including plating, culture techniques, separation by size, centrifugation, staining, and screening. Learn about factors to consider during isolation and the importance of purification processes.
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