Isolation Techniques in Microbiology
39 Questions
3 Views

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to Lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

Which technique is used to quantify enzymes via immunoreactivity?

  • Immunochemical analysis (correct)
  • CHROMOGENIC MEDIA
  • Biochemical Analytical Methods
  • Molecular Methods

What is the principle behind Reverse Genomics?

  • Rapid amplification of nucleic acids
  • Analyzing microbial composition via migration patterns
  • Identifying microorganisms based on morphology and motion
  • Working backwards from observed phenotypes to identify the underlying genetic basis (correct)

Which technique is used for purification of microbial isolates?

  • Gel Electrophoresis
  • Microfluidic Chips
  • Fluorescence-activated cell sorting (FACS) (correct)
  • Chromogenic Media

Which microscopy technique uses dyes for easier and more specific visualization of microorganisms?

<p>Gram staining (C)</p> Signup and view all the answers

Which molecular technique is used for rapid amplification of nucleic acids?

<p>PCR (A)</p> Signup and view all the answers

Which technique is used for screening microorganisms with bioactivity?

<p>Chromogenic Media (A)</p> Signup and view all the answers

Which molecular technique is used for identifying diverse range of bacterial species?

<p>RAPD-PCR (B)</p> Signup and view all the answers

Which biochemical analytical method is used for analysis of non-volatile or thermally labile high molecular compounds?

<p>HPLC-based methods (C)</p> Signup and view all the answers

Which of the following is a technique used for separating microorganisms based on their size and density?

<p>Differential centrifugation (C)</p> Signup and view all the answers

Which of the following staining techniques is commonly used to differentiate between gram-positive and gram-negative bacteria?

<p>Gram staining (D)</p> Signup and view all the answers

What is the primary purpose of using lactophenol blue staining in microscopy?

<p>To stain fungal chitin cell walls (D)</p> Signup and view all the answers

Which of the following is an example of a direct assay for screening microorganisms with bioactivity?

<p>Absorbance spectroscopy (D)</p> Signup and view all the answers

What is the primary purpose of using a moist chamber culture during the isolation and purification of microorganisms?

<p>To maintain a high humidity environment for microbial growth (C)</p> Signup and view all the answers

Which of the following techniques is used to separate microorganisms based on their ability to form colonies on a solid growth medium?

<p>Agar plating (D)</p> Signup and view all the answers

Which of the following assays is used to determine the minimum inhibitory concentration (MIC) of an antimicrobial agent against a microorganism?

<p>Broth dilution method (C)</p> Signup and view all the answers

What is the primary purpose of using a time-kill test or time-kill curve assay during the screening of microorganisms with bioactivity?

<p>To determine the bactericidal or fungicidal effect of an antimicrobial agent over time (A)</p> Signup and view all the answers

What is the main purpose of selective media in microbiology?

<p>To encourage the growth of specific microorganisms while inhibiting others (C)</p> Signup and view all the answers

What technique is used to reduce the number of microbial species for plating techniques?

<p>Serial Dilution Method (A)</p> Signup and view all the answers

Which method involves atomizing a liquid cell suspension with sterile air to scatter cells onto an agar plate?

<p>Cell Spray (D)</p> Signup and view all the answers

What is the disadvantage of spread plating as a culture-dependent method?

<p>Formation of coalesced colonies (A)</p> Signup and view all the answers

Which type of media is supplemented with nutritious materials to encourage the growth of a specific group of microorganisms?

<p>Enriched media (A)</p> Signup and view all the answers

What is the purpose of differential media in microbiology?

<p>To visually distinguish between different types of microorganisms (D)</p> Signup and view all the answers

Which method allows for the separation of particulate contents based on size and shape?

<p>&quot;Filtration&quot; (B)</p> Signup and view all the answers

Which of the following techniques allows for the cultivation of microbial cells requiring interspecific/intraspecific interactions?

<p>Hollow-fiber membrane chamber (HFMC) (D)</p> Signup and view all the answers

What is the principle behind the Reverse Genomics technique?

<p>Utilization of engineered genome-informed antibody to capture specific microorganisms from complex microbial communities (C)</p> Signup and view all the answers

Which technique is commonly combined with fluorescence-activated cell sorting (FACS) for cell sorting?

<p>Live-Fluorescent in situ hybridization (FISH) (C)</p> Signup and view all the answers

Which technique involves the inoculation of a mix of environmental cells and the diffusion of nutrients from the environment?

<p>Diffusion chamber (B)</p> Signup and view all the answers

What is the purpose of the iChip technique?

<p>To produce pure cultures by isolating single cells in individual chambers (A)</p> Signup and view all the answers

Which microscopy technique is used to stain and observe bacterial cell walls?

<p>Gram staining (B)</p> Signup and view all the answers

Which technique involves the use of a dye to stain and observe fungal structures?

<p>Lactophenol Blue Staining (C)</p> Signup and view all the answers

Which technique is used to screen for microorganisms with bioactive compounds?

<p>Bioassay screening (D)</p> Signup and view all the answers

Which technique is used to sort cells based on their specific light scattering and fluorescent characteristics?

<p>Fluorescence-activated cell sorting (FACS) (B)</p> Signup and view all the answers

Which technique is commonly used for photophysiological studies of phytoplankton?

<p>Optical Tweezers (A)</p> Signup and view all the answers

Which technique is used for the isolation of flagellates that exhibit phototaxis?

<p>Phototaxis (A)</p> Signup and view all the answers

Which technique is used for single-cell isolation while avoiding cell damage?

<p>Micropipette (D)</p> Signup and view all the answers

Which technique is used for the separation of cells according to size and density, and the concentration of larger cells?

<p>Polycarbonate filter Centrifugation (B)</p> Signup and view all the answers

Which technique is used to count and analyze the optical properties of cells?

<p>Flow cytometry (B)</p> Signup and view all the answers

Which microscopy technique involves staining cells with a blue dye to enhance the visibility of certain cellular structures?

<p>Lactophenol Blue Staining (A)</p> Signup and view all the answers

Which technique is used to screen microorganisms for bioactive compounds or enzymes?

<p>Reverse Genomics Principle (B)</p> Signup and view all the answers

More Like This

Use Quizgecko on...
Browser
Browser