Isolation Techniques in Microbiology

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Which technique is used to quantify enzymes via immunoreactivity?

Immunochemical analysis (correct)

CHROMOGENIC MEDIA

Biochemical Analytical Methods

Molecular Methods

What is the principle behind Reverse Genomics?

Rapid amplification of nucleic acids

Analyzing microbial composition via migration patterns

Identifying microorganisms based on morphology and motion

Working backwards from observed phenotypes to identify the underlying genetic basis (correct)

Which technique is used for purification of microbial isolates?

Gel Electrophoresis

Microfluidic Chips

Fluorescence-activated cell sorting (FACS) (correct)

Chromogenic Media

Which microscopy technique uses dyes for easier and more specific visualization of microorganisms?

Gram staining Signup and view all the answers

Which molecular technique is used for rapid amplification of nucleic acids?

PCR Signup and view all the answers

Which technique is used for screening microorganisms with bioactivity?

Chromogenic Media Signup and view all the answers

Which molecular technique is used for identifying diverse range of bacterial species?

RAPD-PCR Signup and view all the answers

Which biochemical analytical method is used for analysis of non-volatile or thermally labile high molecular compounds?

HPLC-based methods Signup and view all the answers

Which of the following is a technique used for separating microorganisms based on their size and density?

Differential centrifugation Signup and view all the answers

Which of the following staining techniques is commonly used to differentiate between gram-positive and gram-negative bacteria?

Gram staining Signup and view all the answers

What is the primary purpose of using lactophenol blue staining in microscopy?

To stain fungal chitin cell walls Signup and view all the answers

Which of the following is an example of a direct assay for screening microorganisms with bioactivity?

Absorbance spectroscopy Signup and view all the answers

What is the primary purpose of using a moist chamber culture during the isolation and purification of microorganisms?

To maintain a high humidity environment for microbial growth Signup and view all the answers

Which of the following techniques is used to separate microorganisms based on their ability to form colonies on a solid growth medium?

Agar plating Signup and view all the answers

Which of the following assays is used to determine the minimum inhibitory concentration (MIC) of an antimicrobial agent against a microorganism?

Broth dilution method Signup and view all the answers

What is the primary purpose of using a time-kill test or time-kill curve assay during the screening of microorganisms with bioactivity?

To determine the bactericidal or fungicidal effect of an antimicrobial agent over time Signup and view all the answers

What is the main purpose of selective media in microbiology?

To encourage the growth of specific microorganisms while inhibiting others Signup and view all the answers

What technique is used to reduce the number of microbial species for plating techniques?

Serial Dilution Method Signup and view all the answers

Which method involves atomizing a liquid cell suspension with sterile air to scatter cells onto an agar plate?

Cell Spray Signup and view all the answers

What is the disadvantage of spread plating as a culture-dependent method?

Formation of coalesced colonies Signup and view all the answers

Which type of media is supplemented with nutritious materials to encourage the growth of a specific group of microorganisms?

Enriched media Signup and view all the answers

What is the purpose of differential media in microbiology?

To visually distinguish between different types of microorganisms Signup and view all the answers

Which method allows for the separation of particulate contents based on size and shape?

"Filtration" Signup and view all the answers

Which of the following techniques allows for the cultivation of microbial cells requiring interspecific/intraspecific interactions?

Hollow-fiber membrane chamber (HFMC) Signup and view all the answers

What is the principle behind the Reverse Genomics technique?

Utilization of engineered genome-informed antibody to capture specific microorganisms from complex microbial communities Signup and view all the answers

Which technique is commonly combined with fluorescence-activated cell sorting (FACS) for cell sorting?

Live-Fluorescent in situ hybridization (FISH) Signup and view all the answers

Which technique involves the inoculation of a mix of environmental cells and the diffusion of nutrients from the environment?

Diffusion chamber Signup and view all the answers

What is the purpose of the iChip technique?

To produce pure cultures by isolating single cells in individual chambers Signup and view all the answers

Which microscopy technique is used to stain and observe bacterial cell walls?

Gram staining Signup and view all the answers

Which technique involves the use of a dye to stain and observe fungal structures?

Lactophenol Blue Staining Signup and view all the answers

Which technique is used to screen for microorganisms with bioactive compounds?

Bioassay screening Signup and view all the answers

Which technique is used to sort cells based on their specific light scattering and fluorescent characteristics?

Fluorescence-activated cell sorting (FACS) Signup and view all the answers

Which technique is commonly used for photophysiological studies of phytoplankton?

Optical Tweezers Signup and view all the answers

Which technique is used for the isolation of flagellates that exhibit phototaxis?

Phototaxis Signup and view all the answers

Which technique is used for single-cell isolation while avoiding cell damage?

Micropipette Signup and view all the answers

Which technique is used for the separation of cells according to size and density, and the concentration of larger cells?

Polycarbonate filter Centrifugation Signup and view all the answers

Which technique is used to count and analyze the optical properties of cells?

Flow cytometry Signup and view all the answers

Which microscopy technique involves staining cells with a blue dye to enhance the visibility of certain cellular structures?

Lactophenol Blue Staining Signup and view all the answers

Which technique is used to screen microorganisms for bioactive compounds or enzymes?

Reverse Genomics Principle Signup and view all the answers

Isolation Techniques in Microbiology

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Questions and Answers

Which technique is used to quantify enzymes via immunoreactivity?

What is the principle behind Reverse Genomics?

Which technique is used for purification of microbial isolates?

Which microscopy technique uses dyes for easier and more specific visualization of microorganisms?

Which molecular technique is used for rapid amplification of nucleic acids?

Which technique is used for screening microorganisms with bioactivity?

Which molecular technique is used for identifying diverse range of bacterial species?

Which biochemical analytical method is used for analysis of non-volatile or thermally labile high molecular compounds?

Which of the following is a technique used for separating microorganisms based on their size and density?

Which of the following staining techniques is commonly used to differentiate between gram-positive and gram-negative bacteria?

What is the primary purpose of using lactophenol blue staining in microscopy?

Which of the following is an example of a direct assay for screening microorganisms with bioactivity?

What is the primary purpose of using a moist chamber culture during the isolation and purification of microorganisms?

Which of the following techniques is used to separate microorganisms based on their ability to form colonies on a solid growth medium?

Which of the following assays is used to determine the minimum inhibitory concentration (MIC) of an antimicrobial agent against a microorganism?

What is the primary purpose of using a time-kill test or time-kill curve assay during the screening of microorganisms with bioactivity?

What is the main purpose of selective media in microbiology?

What technique is used to reduce the number of microbial species for plating techniques?

Which method involves atomizing a liquid cell suspension with sterile air to scatter cells onto an agar plate?

What is the disadvantage of spread plating as a culture-dependent method?

Which type of media is supplemented with nutritious materials to encourage the growth of a specific group of microorganisms?

What is the purpose of differential media in microbiology?

Which method allows for the separation of particulate contents based on size and shape?

Which of the following techniques allows for the cultivation of microbial cells requiring interspecific/intraspecific interactions?

What is the principle behind the Reverse Genomics technique?

Which technique is commonly combined with fluorescence-activated cell sorting (FACS) for cell sorting?

Which technique involves the inoculation of a mix of environmental cells and the diffusion of nutrients from the environment?

What is the purpose of the iChip technique?

Which microscopy technique is used to stain and observe bacterial cell walls?

Which technique involves the use of a dye to stain and observe fungal structures?

Which technique is used to screen for microorganisms with bioactive compounds?

Which technique is used to sort cells based on their specific light scattering and fluorescent characteristics?

Which technique is commonly used for photophysiological studies of phytoplankton?

Which technique is used for the isolation of flagellates that exhibit phototaxis?

Which technique is used for single-cell isolation while avoiding cell damage?

Which technique is used for the separation of cells according to size and density, and the concentration of larger cells?

Which technique is used to count and analyze the optical properties of cells?

Which microscopy technique involves staining cells with a blue dye to enhance the visibility of certain cellular structures?

Which technique is used to screen microorganisms for bioactive compounds or enzymes?

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