Isolation of Bacterial Species Quiz

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Questions and Answers

Which method involves heating pathological material to separate spores from non-spore species?

  • Biological methods
  • Chemical methods
  • Physical methods (correct)
  • None of the above

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Study Notes

  • Physical methods involve heating pathological material to 70-80°C to separate spores from non-spore species
  • Vegetative forms are destroyed without harming spores
  • Sowings are made on culture media suitable for the development of respective species
  • Chemical methods involve adding bactericidal or bacteriostatic substances to culture medium
  • Substances are inactive for the bacterial species to be isolated
  • Selective media is prepared and used based on this principle
  • Biological methods involve inoculating pathological material in animals sensitive to bacterial species to be isolated
  • Animal organism acts as a biological filter, destroying saprophytic germs at the entrance gate
  • Pathogenic species multiplies, causing death of the animal
  • Pure culture is obtained by sowing samples from corpses on culture media.

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