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Isolating Mutants and Replica Plating in Genetics

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Isolating Mutants and Replica Plating in Genetics

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Questions and Answers

What is the main difference between negative control and positive control in gene regulation?

Negative control shuts down transcription while positive control triggers transcription.

Negative control involves DNA binding of an activator while positive control involves DNA binding of a repressor.

Negative control triggers transcription while positive control shuts down transcription.

Negative control involves DNA binding of a repressor while positive control involves DNA binding of an activator. (correct)

In the lac operon, what is the role of the lacA gene?

It codes for a transcription activator that enhances lactose metabolism.

It codes for the enzyme transacetylase which allows lactose metabolism. (correct)

It codes for an enzyme that degrades lactose to prevent its build-up.

It codes for a repressor protein that inhibits lactose metabolism.

According to the Jacob-Monod model, what induces transcription in the lac operon?

Binding of the repressor to the promoter region.

Interactions between repressor and activator proteins.

Release of the repressor due to lactose binding. (correct)

Export of sugars by the transacetylase enzyme.

What is the function of the enzyme transacetylase in the lac operon?

It protects the cell from harmful sugars by exporting them. (C) Signup and view all the answers

What did Monod hypothesize about the lacI gene in relation to negative control?

It codes for a repressor protein that exerts negative control over other genes. (A) Signup and view all the answers

What did Jacob and Monod call a set of coordinately regulated bacterial genes transcribed together into one mRNA?

'Operon' (D) Signup and view all the answers

Which statement best describes the role of activators in gene regulation?

Activators bind to DNA and trigger transcription. (C) Signup and view all the answers

'Mechanisms of Negative Control' involve which process according to the text?

'Lactose-Induced Repression' (D) Signup and view all the answers

What hypothesis did Jacob and Monod propose regarding lac operon regulation?

Lactose binds directly to the repressor to induce transcription (A) Signup and view all the answers