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Isolating Mutants and Replica Plating in Genetics
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Isolating Mutants and Replica Plating in Genetics

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Questions and Answers

What is the main difference between negative control and positive control in gene regulation?

  • Negative control shuts down transcription while positive control triggers transcription.
  • Negative control involves DNA binding of an activator while positive control involves DNA binding of a repressor.
  • Negative control triggers transcription while positive control shuts down transcription.
  • Negative control involves DNA binding of a repressor while positive control involves DNA binding of an activator. (correct)
  • In the lac operon, what is the role of the lacA gene?

  • It codes for a transcription activator that enhances lactose metabolism.
  • It codes for the enzyme transacetylase which allows lactose metabolism. (correct)
  • It codes for an enzyme that degrades lactose to prevent its build-up.
  • It codes for a repressor protein that inhibits lactose metabolism.
  • According to the Jacob-Monod model, what induces transcription in the lac operon?

  • Binding of the repressor to the promoter region.
  • Interactions between repressor and activator proteins.
  • Release of the repressor due to lactose binding. (correct)
  • Export of sugars by the transacetylase enzyme.
  • What is the function of the enzyme transacetylase in the lac operon?

    <p>It protects the cell from harmful sugars by exporting them.</p> Signup and view all the answers

    What did Monod hypothesize about the lacI gene in relation to negative control?

    <p>It codes for a repressor protein that exerts negative control over other genes.</p> Signup and view all the answers

    What did Jacob and Monod call a set of coordinately regulated bacterial genes transcribed together into one mRNA?

    <p>'Operon'</p> Signup and view all the answers

    Which statement best describes the role of activators in gene regulation?

    <p>Activators bind to DNA and trigger transcription.</p> Signup and view all the answers

    'Mechanisms of Negative Control' involve which process according to the text?

    <p>'Lactose-Induced Repression'</p> Signup and view all the answers

    What hypothesis did Jacob and Monod propose regarding lac operon regulation?

    <p>Lactose binds directly to the repressor to induce transcription</p> Signup and view all the answers

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