Irreversible Inhibitors and Transition State Analogs Quiz

In Competitive Inhibition, the inhibitor and the substrate compete for binding to the ______ site

In Noncompetitive Inhibition, the inhibitor binds to a site outside of the substrate site, preventing ______

Irreversible inhibition can occur when the inhibitor reacts with and covalently changes the enzyme, killing its ______

Uncompetitive inhibitors only bind to the ES complex, affecting ______ but not substrate binding

Reversible modes of inhibition include competitive, noncompetitive, and ______ inhibition

Irreversible inhibitors, such as suicide substrates, react irreversibly with the reactive R-groups that mediate the enzyme ______

In Uncompetitive Inhibition, the Vmax is lower but the apparent Km is also ______

Adding much more substrate in Competitive Inhibition can outcompete the inhibitor to achieve the same ______

The Uncompetitive Inhibitor will only bind to the ES complex, slowing the transition from ES to EP, resulting in a lower ______

Covalent modifications are reversible means of affecting an enzyme’s ______

Regulatory enzymes are targets of regulatory mechanisms; their allosteric regulation is indicated by ______ kinetics

DIPF (Di-IsoPropylFluorophosphate) is a potent neurotoxin developed in WWII as a chemical warfare agent. The Serine in the active site attacks the exposed __________ electrophile forming a very stable covalent Phospho-Serine bond.

The Transition State analogs bind so tightly that they are effectively __________ Inhibitors

Group-Specific Inhibitors are Irreversible Inhibitors that target specific groups in the catalytic site and form a covalent intermediate that kills the enzyme; e.g., Nerve Gas killing of __________

Aspartate Transcarbamoylase and Glycogen Phosphorylase are good examples of regulatory enzymes that are regulated at multiple levels to yield finely-tuned biological ______

Suicide Substrate Inhibitors are a special class of irreversible inhibitors that enter the catalytic site and partial catalysis creates an active intermediate that covalently kills the enzyme. These inhibitors are a type of __________ Inhibition.

Reversible Inhibition implies that the binding of the inhibitor to the enzyme is not permanent or possible to outcompete by addition of more substrate. There are multiple forms of Reversible Inhibition: Competitive Inhibition, Uncompetitive Inhibition, Mixed Inhibition, and __________ Inhibition.

In Competitive Inhibition, Substrate and Inhibitor compete for binding to the same site on the enzyme. In Uncompetitive Inhibition, the inhibitor will ONLY bind to the ES complex. In Mixed and Non-Competitive Inhibition, the inhibitor will bind to either E or ES; Noncompetitive is a special case of __________ Inhibition.