Ion Exchange Chromatography for Amino Acid Separation
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Questions and Answers

What is the initial condition under which the amino acids are bound to the ion exchange column?

  • Alkaline conditions
  • Acidic conditions (correct)
  • Basic conditions
  • Neutral conditions
  • What is the purpose of applying solutions of increasing ionic strength and pH?

  • To change the pH of the column
  • To release the amino acids from the column (correct)
  • To combine the amino acids into one mixture
  • To separate the amino acids from each other
  • What is the function of the insoluble ion exchanger in the column?

  • To provide a surface for the amino acids to bind to (correct)
  • To separate the amino acids from each other
  • To release the amino acids from the column
  • To change the pH of the column
  • What is the result of applying acidic conditions to the amino acid mixture?

    <p>The amino acids have a net positive charge</p> Signup and view all the answers

    What is the ultimate goal of the ion exchange chromatography process?

    <p>To separate the amino acids from each other</p> Signup and view all the answers

    Quale es le technica de centrifugation que separa particles in functión de lor velocidad de sedimentation?

    <p>Rate-zonal centrifugation</p> Signup and view all the answers

    Qual es le methodo que utilisa gradients de densitate pro separar molecules?

    <p>Isopycnic centrifugation</p> Signup and view all the answers

    Quale es le technica que utilisa un gel pro separar proteins in fonction de lor peso molecular?

    <p>SDS PAGE</p> Signup and view all the answers

    Qual es le methodo que remove small molecules from a solution?

    <p>Dialysis</p> Signup and view all the answers

    Quale es le technica que separa molecules in fonction de lor interaction con un columna?

    <p>Chromatography</p> Signup and view all the answers

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