Ion Exchange Chromatography for Amino Acid Separation

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Questions and Answers

What is the initial condition under which the amino acids are bound to the ion exchange column?

  • Alkaline conditions
  • Acidic conditions (correct)
  • Basic conditions
  • Neutral conditions

What is the purpose of applying solutions of increasing ionic strength and pH?

  • To change the pH of the column
  • To release the amino acids from the column (correct)
  • To combine the amino acids into one mixture
  • To separate the amino acids from each other

What is the function of the insoluble ion exchanger in the column?

  • To provide a surface for the amino acids to bind to (correct)
  • To separate the amino acids from each other
  • To release the amino acids from the column
  • To change the pH of the column

What is the result of applying acidic conditions to the amino acid mixture?

<p>The amino acids have a net positive charge (A)</p> Signup and view all the answers

What is the ultimate goal of the ion exchange chromatography process?

<p>To separate the amino acids from each other (A)</p> Signup and view all the answers

Quale es le technica de centrifugation que separa particles in functión de lor velocidad de sedimentation?

<p>Rate-zonal centrifugation (C)</p> Signup and view all the answers

Qual es le methodo que utilisa gradients de densitate pro separar molecules?

<p>Isopycnic centrifugation (A)</p> Signup and view all the answers

Quale es le technica que utilisa un gel pro separar proteins in fonction de lor peso molecular?

<p>SDS PAGE (D)</p> Signup and view all the answers

Qual es le methodo que remove small molecules from a solution?

<p>Dialysis (A)</p> Signup and view all the answers

Quale es le technica que separa molecules in fonction de lor interaction con un columna?

<p>Chromatography (C)</p> Signup and view all the answers

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