Ion Exchange Chromatography for Amino Acid Separation

Ion Exchange Chromatography for Amino Acid Separation

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@SpectacularGyrolite1083

Questions and Answers

What is the initial condition under which the amino acids are bound to the ion exchange column?

Acidic conditions

What is the purpose of applying solutions of increasing ionic strength and pH?

To release the amino acids from the column

What is the function of the insoluble ion exchanger in the column?

To provide a surface for the amino acids to bind to

What is the result of applying acidic conditions to the amino acid mixture?

<p>The amino acids have a net positive charge</p> Signup and view all the answers

What is the ultimate goal of the ion exchange chromatography process?

<p>To separate the amino acids from each other</p> Signup and view all the answers

Quale es le technica de centrifugation que separa particles in functión de lor velocidad de sedimentation?

<p>Rate-zonal centrifugation</p> Signup and view all the answers

Qual es le methodo que utilisa gradients de densitate pro separar molecules?

<p>Isopycnic centrifugation</p> Signup and view all the answers

Quale es le technica que utilisa un gel pro separar proteins in fonction de lor peso molecular?

<p>SDS PAGE</p> Signup and view all the answers

Qual es le methodo que remove small molecules from a solution?

<p>Dialysis</p> Signup and view all the answers

Quale es le technica que separa molecules in fonction de lor interaction con un columna?

<p>Chromatography</p> Signup and view all the answers

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