Introduction to Histology

The study of the microscopic structure of cells, tissues, and organs in the body.

An instrument with magnifying lenses to view objects too small for the naked eye.

The microscope's ability to distinguish fine details in an image.

The enlargement of an object's image.

Uses light to view thin, stained tissue sections.

Uses electron beams for higher resolution than LM.

Uses electron beams passing through ultrathin tissue sections.

Images surface of cells, tissues, and organs in 3D.

Thin slices of tissues prepared for microscopic examination.

Processes used to enhance contrast in tissue samples to make features easier to see under a microscope.

Epithelial tissues viewed under a light microscope (LM).

Epithelial tissues viewed under a transmission electron microscope (TEM).

Epithelial tissues viewed under a scanning electron microscope (SEM).

A microscope that uses visible light as its source of illumination.

Uses electrons as its source of illumination, offering much higher resolution and magnification.

The ability of a microscope to distinguish between two closely spaced points.

The ability of a microscope to enlarge the image of a specimen.

The process of preparing thin tissue samples for microscopic examination (LM & EM).

Common method for preparing tissue sections to be viewed under a light microscope (LM).

A rapid method for preparing tissue sections to be viewed under a light microscope (LM).

Used to prepare very thin (=ultrathin) tissue sections for use in electron microscopy (TEM).

A basic dye used in staining that stains acidic components blue (e.g., DNA and RNA).

An acidic dye used in staining that stains basic components pink (e.g., proteins in the cytoplasm).

Methods used to reveal the general structure of tissues.

Methods used to show specific tissue components like lipids, carbohydrates, or others.

A special stain used to highlight lipids in tissues.

A histochemical reaction that stains carbohydrates, especially glycogen.

Cell components are stained by acidic dyes.

A general stain used frequently during microscopic analysis of cells.

Different types of microscopes based on their functions and how they work

Types of microscopes using beams of electrons, like TEM & SEM for higher resolution.

Functions of a microscope include magnification and resolution of tiny structures.

Different ways to slice tissue for microscope viewing. (e.g. thin and thick)

Various methods of staining, like Hematoxylin and Eosin, for better visualization.

Key differences in how they work (light vs. electron beams) & resolution.

Electron microscopes use magnetic lenses.

Light microscope resolution primarily depends on the objective lens.

Thin stained tissue sections are used for light microscopy.

Microscopy functions include magnification and resolution.

Periodic acid-Schiff (PAS) reaction stains glycogen.

The resolution of light microscopes is about 0.2 micrometers.

Sudan black is a special staining method.

PAS reaction is a histochemical staining method.

Magnification is the enlargement of an object's image.

Resolution is the ability to distinguish fine details in an image.

Scanning electron microscopes provide 3D images of surfaces.

Sudan black stains lipids.