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Questions and Answers
Who is described as the 'Father of Hematology' and the inventor of the microscope for the microscopic size of RBC?
Who is described as the 'Father of Hematology' and the inventor of the microscope for the microscopic size of RBC?
- Anton van Leeuwenhoek (correct)
- Giulio Bizzozero
- Athanasius Kircher
- James Homer Wright
What is the approximate total volume of blood in an average male?
What is the approximate total volume of blood in an average male?
- 6-7 L
- 3-4 L
- 2-3 L
- 5-6 L (correct)
Which component of blood is approximately 3.5-4.5 times thicker than water?
Which component of blood is approximately 3.5-4.5 times thicker than water?
- Buffy coat
- Erythrocyte (correct)
- Formed elements
- Plasma
What is the function of the uppermost layer of the Buffy coat?
What is the function of the uppermost layer of the Buffy coat?
In which year did Athanasius Kircher describe a worm in the blood?
In which year did Athanasius Kircher describe a worm in the blood?
What does 'in vivo' research refer to?
What does 'in vivo' research refer to?
What is the specific gravity of arterial (oxygenated) blood?
What is the specific gravity of arterial (oxygenated) blood?
What is the mode of action of ethylenediaminetetraacetic acid (EDTA) as an anticoagulant?
What is the mode of action of ethylenediaminetetraacetic acid (EDTA) as an anticoagulant?
Which anticoagulant is the choice for most chemistry tests?
Which anticoagulant is the choice for most chemistry tests?
What is the ratio of blood to anticoagulant for the Standard Westergren (black top) method?
What is the ratio of blood to anticoagulant for the Standard Westergren (black top) method?
Which anticoagulant is used for preserving glucose and as an inhibitor of glycolysis?
Which anticoagulant is used for preserving glucose and as an inhibitor of glycolysis?
What is the action of sodium fluoride as an anticoagulant?
What is the action of sodium fluoride as an anticoagulant?
What did Giulio Bizzozero describe in the 1800s?
What did Giulio Bizzozero describe in the 1800s?
What is the approximate total volume of blood in an average female?
What is the approximate total volume of blood in an average female?
Who is considered the 'Father of Hematology' and the inventor of the microscope for the microscopic size of RBC?
Who is considered the 'Father of Hematology' and the inventor of the microscope for the microscopic size of RBC?
What is the percentage composition of the solid portion in blood?
What is the percentage composition of the solid portion in blood?
What is the approximate pH range of blood?
What is the approximate pH range of blood?
What does 'in vivo' research refer to?
What does 'in vivo' research refer to?
What is the specific gravity range for serum?
What is the specific gravity range for serum?
What is the mode of action of EDTA as an anticoagulant?
What is the mode of action of EDTA as an anticoagulant?
Which anticoagulant is the choice for most chemistry tests?
Which anticoagulant is the choice for most chemistry tests?
What is the action of sodium fluoride as an anticoagulant?
What is the action of sodium fluoride as an anticoagulant?
What is the function of the anticoagulant EDTA?
What is the function of the anticoagulant EDTA?
Which anticoagulant is recommended for the Standard Westergren (black top) method?
Which anticoagulant is recommended for the Standard Westergren (black top) method?
What is the optimum concentration of heparin for most chemistry tests?
What is the optimum concentration of heparin for most chemistry tests?
Which anticoagulant is used in a concentration of $3.2%$ or $0.109M$ and preserves factor V and VIII?
Which anticoagulant is used in a concentration of $3.2%$ or $0.109M$ and preserves factor V and VIII?
What is the ratio of blood to anticoagulant for the Standard Westergren (black top) method?
What is the ratio of blood to anticoagulant for the Standard Westergren (black top) method?
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Study Notes
Blood and Hematology
- Antonie Van Leeuwenhoek is described as the 'Father of Hematology' and the inventor of the microscope for the microscopic size of RBC.
Blood Composition
- The approximate total volume of blood in an average male is 5-6 liters.
- The approximate total volume of blood in an average female is 4-5 liters.
- The solid portion of blood makes up around 45% of the total volume.
Blood Properties
- The specific gravity of arterial (oxygenated) blood is 1.056-1.066.
- The specific gravity range for serum is 1.018-1.022.
- The approximate pH range of blood is 7.35-7.45.
Anticoagulants
- The component of blood that is approximately 3.5-4.5 times thicker than water is plasma.
- Ethylenediaminetetraacetic acid (EDTA) is an anticoagulant that works by binding to calcium ions, preventing blood clotting.
- EDTA is the choice for most chemistry tests.
- The ratio of blood to anticoagulant for the Standard Westergren (black top) method is 4:1.
- Sodium fluoride is an anticoagulant that inhibits glycolysis and preserves glucose.
- Sodium fluoride is used as an anticoagulant in a concentration of 2-3%.
- Heparin is an anticoagulant that works by activating antithrombin, and its optimum concentration for most chemistry tests is 10-15 units/mL.
- Citrate is an anticoagulant used in a concentration of 3.2% or 0.109M and preserves factor V and VIII.
History of Hematology
- Athanasius Kircher described a worm in the blood in 1658.
- Giulio Bizzozero described platelets in the 1800s.
Research
- 'In vivo' research refers to experiments or observations conducted within living organisms, such as animals or humans.
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