Podcast
Questions and Answers
What is the purpose of gene cloning?
What is the purpose of gene cloning?
- To introduce a foreign DNA molecule into a replicating cell
- To cut DNA with restriction enzymes
- To amplify the production of DNA fragments (correct)
- To isolate and purify a single DNA fragment
What is the role of Southern blot technique in gene cloning?
What is the role of Southern blot technique in gene cloning?
- Separating DNA fragments by Agarose gel electrophoresis
- Cutting DNA with restriction enzymes
- Transferring DNA from the fragile gel to a nylon sheet and hybridizing with a probe (correct)
- Purifying DNA from cells
What is the function of an expression vector in gene cloning?
What is the function of an expression vector in gene cloning?
- To cut DNA with restriction enzymes
- To introduce a specific gene into a target cell and produce the encoded protein (correct)
- To separate DNA fragments by Agarose gel electrophoresis
- To isolate and purify a single DNA fragment
What is the purpose of the origin of replication in an expression vector (plasmid)?
What is the purpose of the origin of replication in an expression vector (plasmid)?
Which feature is crucial for identifying colonies containing the gene of interest in a recombinant plasmid?
Which feature is crucial for identifying colonies containing the gene of interest in a recombinant plasmid?
What is the purpose of the transcription termination sequence in an expression vector (plasmid)?
What is the purpose of the transcription termination sequence in an expression vector (plasmid)?
Why is a strong termination codon important in an expression vector (plasmid)?
Why is a strong termination codon important in an expression vector (plasmid)?
What is the purpose of the polylinker in an expression vector (plasmid)?
What is the purpose of the polylinker in an expression vector (plasmid)?
Why is ampicillin used in the identification of colonies containing recombinant plasmids?
Why is ampicillin used in the identification of colonies containing recombinant plasmids?
What technique is used to grow transformed bacterial cells on medium containing ampicillin?
What technique is used to grow transformed bacterial cells on medium containing ampicillin?
How are successful insertions identified in Blue/White screening using Xgal substrate?
How are successful insertions identified in Blue/White screening using Xgal substrate?
