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Introduction to Gene Cloning

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Questions and Answers

What is the purpose of gene cloning?

To introduce a foreign DNA molecule into a replicating cell

To cut DNA with restriction enzymes

To amplify the production of DNA fragments (correct)

To isolate and purify a single DNA fragment

What is the role of Southern blot technique in gene cloning?

Separating DNA fragments by Agarose gel electrophoresis

Cutting DNA with restriction enzymes

Transferring DNA from the fragile gel to a nylon sheet and hybridizing with a probe (correct)

Purifying DNA from cells

What is the function of an expression vector in gene cloning?

To cut DNA with restriction enzymes

To introduce a specific gene into a target cell and produce the encoded protein (correct)

To separate DNA fragments by Agarose gel electrophoresis

To isolate and purify a single DNA fragment

What is the purpose of the origin of replication in an expression vector (plasmid)?

To allow the plasmid to replicate independently in the host cell Signup and view all the answers

Which feature is crucial for identifying colonies containing the gene of interest in a recombinant plasmid?

Blue/White screening using Xgal substrate Signup and view all the answers

What is the purpose of the transcription termination sequence in an expression vector (plasmid)?

To terminate transcription of the inserted gene Signup and view all the answers

Why is a strong termination codon important in an expression vector (plasmid)?

To terminate transcription of the inserted gene Signup and view all the answers

What is the purpose of the polylinker in an expression vector (plasmid)?

To provide a site for gene insertion Signup and view all the answers

Why is ampicillin used in the identification of colonies containing recombinant plasmids?

To provide antibiotic resistance for transformed cells containing plasmids Signup and view all the answers

What technique is used to grow transformed bacterial cells on medium containing ampicillin?

Chemical Transformation using Calcium Chloride Signup and view all the answers

How are successful insertions identified in Blue/White screening using Xgal substrate?

LacZ(-) white colonies Signup and view all the answers