11 Questions
What is the purpose of gene cloning?
To amplify the production of DNA fragments
What is the role of Southern blot technique in gene cloning?
Transferring DNA from the fragile gel to a nylon sheet and hybridizing with a probe
What is the function of an expression vector in gene cloning?
To introduce a specific gene into a target cell and produce the encoded protein
What is the purpose of the origin of replication in an expression vector (plasmid)?
To allow the plasmid to replicate independently in the host cell
Which feature is crucial for identifying colonies containing the gene of interest in a recombinant plasmid?
Blue/White screening using Xgal substrate
What is the purpose of the transcription termination sequence in an expression vector (plasmid)?
To terminate transcription of the inserted gene
Why is a strong termination codon important in an expression vector (plasmid)?
To terminate transcription of the inserted gene
What is the purpose of the polylinker in an expression vector (plasmid)?
To provide a site for gene insertion
Why is ampicillin used in the identification of colonies containing recombinant plasmids?
To provide antibiotic resistance for transformed cells containing plasmids
What technique is used to grow transformed bacterial cells on medium containing ampicillin?
Chemical Transformation using Calcium Chloride
How are successful insertions identified in Blue/White screening using Xgal substrate?
LacZ(-) white colonies
Learn about the basics of gene cloning, including what it is, its elements, and the steps involved in successful gene cloning such as insertion, transformation, and identification.
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